Versatile Separation and Analysis of Heparan Sulfate Oligosaccharides using Graphitised Carbon Liquid Chromatography and Electrospray Mass Spectrometry

Miller R, Guimond S, Prescott M, Karlsson N, Turnbull JE, Anal Chem, 2017, (1): 1 - 23

PubMed Entry:

Retention Time Recorded Precursor Mass Structure
18.0 645.5 [M -2H]2-
18.5 795.1 [M -1H]1-
20.0 592.1 [M -1H]1-
20.0 645.5 [M -2H]2-
21.0 645.5 [M -2H]2-
21.0 477.0 [M -2H]2-
21.5 795.1 [M -1H]1-
22.0 645.5 [M -2H]2-

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Sus Scrofa
Tissue - Intestine, Small

HPLC

Column ProteoCol

Method

  • 40 gradient with 4 run rate
  • 10 mM Ammonium Bicarbonate
  • 80% acetonitrile in 10 mM Ammonium Bicarbonate

Mass Spec

Agilent XCT Ultra ESI

UniCarb-DB data licensed under Creative Commons License CC BY 4.0

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