Haemocytes and plasma of the eastern oyster (Crassostrea virginica) displays a diverse repertoire of sulphated and blood group A-modified N-glycans

Kurz S, Jin C, Hykollari A, Gregorich D, Giomarelli B, Vasta GR, Wilson IAB and Paschinger K, J Biol Chem, 2013, (288): 24410 - 24428

PubMed Entry:

Retention Time Recorded Precursor Mass Structure
26.29 1276.33 [M -1H]1-
27.05 1128.33 [M -1H]1-
27.26 1260.33 [M -1H]1-
28.1 1290.42 [M -1H]1-
28.1 1422.42 [M -1H]1-
29.28 899.42 [M -2H]2-
29.28 1274.5 [M -1H]1-
29.98 1370.42 [M -1H]1-
30.14 1436.5 [M -1H]1-
31.77 1516.42 [M -1H]1-
32.21 1450.5 [M -1H]1-

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Crassostrea Virginica
Tissue - Plasma

HPLC

Column Custom made Hypercarb

Method

  • 40 gradient with 10 run rate
  • 10mM Ammonium Bicarbonate
  • 80% Acetonitrile in 10mM Ammonium Bicarbonate

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

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