O-linked Glycome & Proteome of High Molecular Mass Proteins in Human Ovarian Cancer Ascites: Identification of Sulfation, Disialic Acid and O-linked Fucose

Karlsson NG, McGuckin MA, Glycobiology, 2012, (22): 918 - 929

PubMed Entry:

Retention Time Recorded Precursor Mass Structure
Not recorded 1110.32 [M -2H]2-
Not recorded 888.0 [M -2H]2-
Not recorded 1150.3 [M -2H]2-
Not recorded 927.7 [M -2H]2-
Not recorded 1070.3 [M -2H]2-
Not recorded 1030.36 [M -2H]2-
Not recorded 1372.345 [M -2H]2-
Not recorded 1332.86 [M -2H]2-
Not recorded 1292.886 [M -2H]2-
Not recorded 1252.91 [M -2H]2-
Not recorded 1212.9 [M -2H]2-
8.7 384.3 [M -1H]1-
11.8 513.5 [M -1H]1-
13.2 425.3 [M -1H]1-
13.3 697.3 [M -1H]1-
13.3 675.5 [M -1H]1-
13.6 587.4 [M -1H]1-
14.8 667.3 [M -1H]1-
15.5 697.2 [M -1H]1-
15.5 675.4 [M -1H]1-
16.2 988.3 [M -2H]2-
16.2 966.4 [M -2H]2-
16.2 482.6 [M -2H]2-
16.5 749.3 [M -1H]1-
17.0 829.3 [M -1H]1-
17.2 1257.0 [M -1H]1-
17.4 530.3 [M -1H]1-
17.6 878.5 [M -1H]1-
17.6 900.4 [M -1H]1-
18.9 958.4 [M -1H]1-
18.9 478.8 [M -1H]1-
18.9 980.3 [M -1H]1-
19.8 519.9 [M +(Na-H) -1H]1-
19.8 1040.5 [M +(Na-H) -1H]1-
19.8 1962.8 [M +(Na-H) -1H]1-
20.9 1142.3 [M -1H]1-
20.9 559.7 [M -1H]1-
20.9 1120.5 [M -1H]1-
21.4 850.8 [M -2H]2-
21.9 1120.5 [M -1H]1-
22.0 1040.6 [M -1H]1-
22.8 705.6 [M -3H]3-
22.8 1411.5 [M -3H]3-
22.8 469.7 [M -3H]3-
23.6 821.32 [M -1H]1-
24.0 1353.7 [M -1H]1-
24.0 676.5 [M -1H]1-
24.0 1331.7 [M -1H]1-
24.0 665.7 [M -1H]1-
25.6 705.0 [M -2H]2-
25.6 665.2 [M -2H]2-
27.0 1033.3 [M -2H]2-
29.5 848.7 [M -2H]2-

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Homo Sapiens
Tissue - Ascitic Fluid

HPLC

Column Custom made Hypercarb

Method

  • 60 gradient with 10 run rate
  • 10mM Ammonium Bicarbonate
  • 80% Acetonitrile in 10mM Ammonium Bicarbonate

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

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