O-linked Glycome & Proteome of High Molecular Mass Proteins in Human Ovarian Cancer Ascites: Identification of Sulfation, Disialic Acid and O-linked Fucose

PubMed Entry

GlyToucan Accession Number: G08140FC

Peak Intensity
289.1 30.0
290.1 2446.0

Annotations:

B
291.0 580.0
292.1 38.0

Annotations:

C^{0,3}X_{Gal} C^{1,4}X_{Gal} ^{1,4}A_{GlcNAc}
299.2 18.0
302.5 32.0
305.0 25.0

Annotations:

Y^{0,2}X_{GlcNAc}
312.0 103.0
323.7 21.0

Annotations:

^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y
325.1 38.0
329.3 31.0
334.2 16.0
347.2 162.0

Annotations:

Z^{2,4}X_{GlcNAc}
348.1 97.0

Annotations:

ZZ
358.1 33.0
364.1 313.0

Annotations:

B
365.1 159.0

Annotations:

Y^{2,4}X_{GlcNAc}
366.1 211.0

Annotations:

YZ ZY
367.4 15.0
370.2 13.0
371.2 41.0
377.2 18.0
379.9 39.0

Annotations:

^{1,4}A_{Gal}
382.2 49.0

Annotations:

C
384.1 138.0

Annotations:

YY
385.1 36.0
386.2 37.0
388.2 36.0
389.2 3430.0

Annotations:

ZZ
390.1 467.0
391.1 75.0
396.0 44.0
404.1 65.0
406.0 20.0
407.1 1080.0

Annotations:

YZ ZY
408.3 267.0

Annotations:

B^{1,3}X_{NeuAc}
412.1 18.0

Annotations:

Y^{1,5}X_{GlcNAc}
416.3 28.0
424.1 358.0

Annotations:

^{1,5}A_{Gal}
425.2 210.0

Annotations:

YY
426.1 33.0

Annotations:

C^{1,3}X_{NeuAc}
434.1 42.0
435.2 68.0

Annotations:

^{1,5}X_{Gal}Z Z^{1,5}X_{Gal}
436.3 14.0

Annotations:

^{0,2}X_{NeuAc}Z
438.2 32.0

Annotations:

^{1,5}X_{NeuAc}Z Z^{1,4}X_{GlcNAc}
449.3 42.0

Annotations:

Z^{0,2}X_{GlcNAc} ^{0,2}X_{Gal}Z Z^{0,2}X_{Gal}
450.0 13.0
452.1 45.0

Annotations:

B
454.1 20.0

Annotations:

^{0,2}X_{NeuAc}Y
455.4 19.0
461.3 36.0
467.1 121.0

Annotations:

Y^{0,2}X_{GlcNAc} ^{0,2}X_{Gal}Y Y^{0,2}X_{Gal}
474.1 23.0
481.2 39.0
485.4 66.0
491.2 111.0
500.1 15.0

Annotations:

^{3,5}X_{NeuAc}Y
503.2 35.0
509.2 1294.0

Annotations:

Z^{2,4}X_{GlcNAc} ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z Z^{0,4}X_{Gal} Z^{1,3}X_{Gal} Z^{2,4}X_{Gal}
510.2 283.0
511.3 71.0
521.1 133.0
522.3 110.0
523.2 41.0
527.3 2383.0

Annotations:

Y^{0,4}X_{Gal} Y^{1,3}X_{Gal} Y^{2,4}X_{GlcNAc} ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y Y^{2,4}X_{Gal}
528.3 766.0
529.2 144.0
530.5 14.0
533.2 145.0
534.5 13.0
541.7 17.0

Annotations:

^{1,4}X_{NeuAc}Z
545.2 468.0
546.2 184.0
551.2 287.0

Annotations:

ZZ
552.4 78.0
563.3 18.0
565.1 24.0
567.3 67.0
569.3 19376.0

Annotations:

Z
570.3 4427.0
571.2 832.0
585.2 41.0
587.3 6240.0

Annotations:

Y
588.3 1736.0
589.3 592.0
590.2 71.0
596.9 44.0

Annotations:

Z^{1,5}X_{Gal}
611.3 20.0

Annotations:

Z^{0,2}X_{Gal}
612.3 35.0
613.1 47.0

Annotations:

^{1,3}X_{NeuAc}Z
613.6 57.0

Annotations:

^{1,3}X_{NeuAc}Z
616.6 96.0
626.2 24.0
627.3 50.0

Annotations:

Z^{2,5}X_{Gal}
628.4 45.0
629.4 54.0

Annotations:

Y^{0,2}X_{Gal} ^{0,2}X_{Gal}
630.5 18.0
631.3 32.0

Annotations:

^{1,3}X_{NeuAc}Y
647.3 129.0
655.3 32.0

Annotations:

^{2,5}X_{NeuAc}Z
656.1 27.0
659.3 25.0

Annotations:

^{1,5}X_{NeuAc}Y Y^{0,3}X_{Gal} Y^{1,4}X_{Gal} ^{1,4}X_{Gal}
662.3 23.0
665.5 30.0
671.2 21.0

Annotations:

Z^{0,4}X_{Gal} Z^{1,3}X_{Gal} Z^{2,4}X_{Gal}
675.2 20.0

Annotations:

Y
680.5 45.0
683.3 13.0
685.5 13.0

Annotations:

^{3,5}X_{NeuAc}Z
689.3 730.0

Annotations:

Y^{0,4}X_{Gal} Y^{1,3}X_{Gal} Y^{2,4}X_{Gal} ^{1,3}X_{Gal} ^{2,4}X_{Gal}
690.3 415.0
691.3 142.0
692.3 34.0
693.4 27.0
697.3 68.0
698.1 26.0
707.3 724.0
708.3 101.0
709.4 71.0
711.2 19.0
718.3 13.0
729.2 25.0
731.3 783.0

Annotations:

Z
732.4 210.0
745.3 901.0

Annotations:

^{1,4}X_{NeuAc}Z
746.3 236.0
747.5 118.0

Annotations:

^{1,4}X_{GlcNAc}
749.3 21215.0

Annotations:

Y
750.3 7278.0
751.3 2552.0
752.3 190.0
756.3 26.0
757.5 21.0
774.0 27.0

Annotations:

^{2,5}X_{GlcNAc}
775.7 13.0
782.4 40.0
784.3 12.0
791.4 302.0
792.3 119.0
800.4 20.0
801.2 93.0
801.9 18.0
802.5 38.0
818.4 22.0

Annotations:

^{2,4}X_{GlcNAc}
819.3 552.0

Annotations:

^{0,2}X_{NeuAc}
820.4 77.0
821.3 86.0

Annotations:

^{1,5}X_{NeuAc}
832.8 13.0
835.2 37.0

Annotations:

^{2,5}X_{NeuAc}
842.4 945.0
843.3 177.0
844.2 79.0
845.5 57.0
853.3 11.0
854.3 22.0
860.1 79.0

Annotations:

Z
872.3 244.0
873.3 39.0
877.3 59.0
878.4 68.0

Annotations:

Y
879.7 14.0
881.3 50.0
884.3 61.0
885.5 31.0
902.3 43.0
916.4 54.0
919.3 15.0
920.6 68.0

Annotations:

^{0,2}X_{Gal} ^{0,4}X_{NeuAc}
921.4 68.0
927.4 23.0
932.3 48.0
933.3 29.0
934.4 14.0
936.7 41.0

Annotations:

^{2,5}X_{Gal}
938.4 33.0
944.4 92.0
945.2 95.0
946.2 29.0
949.7 36.0
950.2 65.0

Annotations:

^{1,4}X_{Gal} ^{0,3}X_{Gal}
952.4 120.0
955.1 14.0
956.4 112.0
962.4 6456.0
963.3 2281.0
964.3 825.0
965.3 70.0
968.4 48.0
969.2 21.0
970.3 26.0
974.5 15.0
976.9 14.0
978.0 47.0
980.4 3660.0

Annotations:

^{0,4}X_{Gal} ^{1,3}X_{Gal} ^{2,4}X_{Gal}
981.3 1317.0
982.3 373.0
983.4 113.0
984.3 19.0
987.4 19.0
988.9 26.0
992.5 37.0
993.9 11.0
996.4 107.0

Annotations:

^{1,3}X_{NeuAc}
997.4 99.0
998.3 51.0
999.1 39.0
1001.3 20.0
1002.9 23.0
1004.3 109.0
1006.5 44.0
1007.3 31.0
1008.3 16.0
1009.7 11.0
1010.9 10.0
1013.6 35.0
1014.4 38.0
1015.0 37.0
1020.3 14.0
1022.4 3779.0
1023.4 2224.0
1024.4 1323.0
1025.4 73.0
1028.0 13.0
1032.1 13.0
1032.6 23.0
1035.3 10.0
1036.7 27.0
1048.3 34.0
1051.3 165.0
1051.9 64.0
1059.4 84.0
1062.4 518.0
1063.3 96.0

There are 13 similar spectra:

Id Structure Score

3089

0.97

2642

0.97

202

0.96

2643

0.96

203

0.94

2817

0.94

132

0.93

3090

0.90

48

0.85

2615

0.78

79

0.76

2682

0.54

80

0.52
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