Negative ion CID fragmentation of O-linked oligosaccharides aldoses-charge induced and charge remote fragmentation

Doohan RA, Hayes CA, Harhen B, Karlsson NG, J Am Soc Mass Spectrom, 2011, (22): 1052 - 1062

PubMed Entry:

Retention Time Recorded Precursor Mass Structure
13.25 675.2 [M -1H]1-
15.2 673.3 [M -1H]1-
15.7 673.3 [M -1H]1-
16.9 964.4 [M -2H]2-
17.0 481.7 [M -2H]2-
21.0 665.2 [M -1H]1-
21.1 1331.4 [M -1H]1-
21.6 675.2 [M -1H]1-
23.3 664.2 [M -1H]1-
23.5 1329.4 [M -1H]1-
26.2 1121.356 [M -1H]1-
26.7 813.2 [M -1H]1-
27.0 1039.1 [M -1H]1-
29.8 1041.3991 [M -1H]1-

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Bos Taurus
Tissue - Serum
Glycoprotein - Alpha-2-HS-glycoprotein
Taxonomy - Sus Scrofa
Tissue - Gastric Mucosa

HPLC

Column Custom made Hypercarb

Method

  • 60 gradient with 10 run rate
  • 10mM Ammonium Bicarbonate
  • 80% Acetonitrile in 10mM Ammonium Bicarbonate

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

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