MIRAGE upload

O-glycan repertoires on a mucin-type reporter protein expressed in CHO cell pools transiently transfected with O-glycan core enzyme cDNAs

GlyToucan Accession Number: G18132YR

Peak	Intensity
216.08	0.3
221.1	0.5
Annotations: ^{2,4}A_{GlcNAc}
247.12	0.3
263.23	1.0
273.84	0.2
304.22	0.5
341.12	0.3
343.03	0.3
345.08	0.4
346.26	0.2
347.17	2.2
348.07	1.0
353.01	0.4
364.15	5.2
Annotations: B
365.21	0.8
366.32	1.7
Annotations: Z
367.13	0.5
371.11	0.5
384.14	1.8
Annotations: Y
389.15	41.5
Annotations: ZZ
390.09	3.4
390.38	2.2
396.15	0.4
407.18	10.1
Annotations: YZ ZY
407.97	0.6
408.36	0.7
424.1	3.9
425.26	0.9
Annotations: YY
426.34	0.3
439.26	0.2
467.25	0.2
474.21	0.5
478.94	0.2
491.29	0.2
498.69	0.2
509.23	7.7
509.95	0.2
510.22	0.5
513.14	0.3
520.67	0.4
521.23	1.7
522.2	0.2
523.14	0.2
527.19	21.7
528.3	3.8
529.93	0.2
531.98	0.2
533.22	1.5
533.82	0.3
539.47	0.2
542.46	0.4
544.97	0.6
545.14	0.3
546.19	0.3
548.83	0.2
551.21	0.9
551.51	0.2
552.26	0.7
556.9	0.3
558.07	0.2
567.09	0.3
569.19	251.8
Annotations: Z Z
570.2	53.3
571.41	0.2
577.17	0.2
587.21	34.1
Annotations: Y Y
588.24	6.8
589.24	0.2
593.9	0.2
596.91	0.4
597.89	0.2
598.42	0.2
603.17	0.2
627.91	0.4
628.87	0.2
630.6	0.2
644.05	0.9
646.14	0.2
646.99	0.8
647.32	1.0
648.37	0.2
649.03	0.2
651.24	0.2
654.09	0.3
664.72	0.2
666.69	0.3
667.81	0.2
671.4	0.3
685.06	0.4
685.71	0.2
687.5	0.4
688.46	0.2
689.24	19.2
690.31	2.8
699.81	0.2
704.96	0.3
705.86	0.3
707.24	14.5
708.32	2.1
715.17	0.2

There are 17 similar spectra:

Id	Structure	Score
187		1.00
3051		1.00
2777		1.00
2675		1.00
100		1.00
24		1.00
3052		0.99
3659		0.98
330		0.97
279		0.96
458		0.95
42		0.93
2778		0.88
188		0.88
76		0.87
25		0.87
43		0.57

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass 748.29
(Calculated Monoisotopic)

Reducing End Reduced

Persubstitution Underivatised

Biological Context

Taxonomy - Cricetulus Griseus

Tissue - Cell Line, Transformed

HPLC

Column Custom made Hypercarb

Method

Run time not recorded
50mM Ammonium Formate pH4.4
Acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

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