In-depth N-glycome profiling of paired colorectal cancer and non-tumorigenic tissues reveals cancer-, stage- and EGFR-specific protein N-glycosylation.

PubMed Entry

GlyToucan Accession Number: G13249MW

Peak Intensity
368.2 183.0

Annotations:

Y
424.1 737.0

Annotations:

^{1,3}A_{Man} ^{0,4}A_{Man}Y C^{0,2}X_{Gal}
466.2 164.0

Annotations:

^{1,3}A_{Man}Z ^{2,4}A_{Man}Z B^{0,4}X_{Gal} B^{1,3}X_{Gal} B^{2,4}X_{Gal} C^{2,5}X_{GlcNAc}
468.3 187.0

Annotations:

C^{2,5}X_{NeuAc}
470.3 104.0

Annotations:

C
503.2 391.0

Annotations:

B^{3,5}X_{NeuAc} Y^{0,3}X_{GlcNAc}
553.3 553.0

Annotations:

Z
554.3 144.0

Annotations:

B^{1,5}X_{GlcNAc} B^{2,4}X_{NeuAc} ^{1,4}A_{Man}Y C^{0,3}X_{Gal} C^{1,4}X_{Gal}
556.7 407.0

Annotations:

YY
571.2 224.0

Annotations:

Y
613.3 102.0

Annotations:

^{0,2}X_{Man}
616.2 134.0

Annotations:

CY
627.8 247.0

Annotations:

^{2,4}A_{GlcNAc}Z
647.5 195.0
655.2 691.0

Annotations:

B
670.3 223.0

Annotations:

BZ
671.2 203.0

Annotations:

^{2,5}A_{Man}Z ^{0,2}X_{GlcNAc}Y
673.3 278.0

Annotations:

C
688.0 230.0

Annotations:

BY CZ
688.4 479.0

Annotations:

BY CZ
772.3 301.0
799.3 123.0

Annotations:

BZ
803.3 216.0

Annotations:

YZ ZY YZ ZY ZY YZ YZ ZY ZY YZ
807.2 114.0
816.8 166.0

Annotations:

BY CZ
818.1 308.0

Annotations:

^{3,5}A_{GlcNAc}Z ^{1,4}A_{GlcNAc}Y ^{1,4}A_{GlcNAc}Y C^{0,3}X_{GlcNAc} C^{1,4}X_{Man} C^{0,3}X_{GlcNAc}
818.4 218.0

Annotations:

^{1,4}A_{GlcNAc}Y ^{1,4}A_{GlcNAc}Y C^{0,3}X_{GlcNAc} C^{1,4}X_{Man} C^{0,3}X_{GlcNAc}
831.4 366.0

Annotations:

^{1,5}A_{GlcNAc}Z ^{1,5}A_{GlcNAc}Z ^{1,5}A_{Man}Z ^{1,5}A_{Man}Z ^{0,3}X_{GlcNAc}Y
835.4 1828.0

Annotations:

CY
836.4 1558.0
846.8 106.0

Annotations:

^{2,4}X_{GlcNAc}Y
875.4 270.0

Annotations:

ZZ ZZ YZ ZY
875.8 240.0

Annotations:

ZZ ZZ YZ ZY
876.8 364.0

Annotations:

YY
877.3 351.0

Annotations:

YY YZ ZY
891.2 108.0

Annotations:

BY BY CZ CZ BY CZ
926.8 283.0

Annotations:

B^{0,2}X_{NeuAc} ^{1,3}X_{Man}Z Z^{0,4}X_{Fuc} ^{1,3}X_{NeuAc}Z Z^{1,5}X_{Fuc}
935.4 174.0

Annotations:

B
936.4 135.0

Annotations:

C^{1,5}X_{NeuAc} ^{2,5}X_{NeuAc}Y Y^{2,5}X_{NeuAc}
936.9 141.0
937.3 319.0
944.4 174.0

Annotations:

C
948.0 707.0

Annotations:

ZZ ZZ
948.9 280.0

Annotations:

Z
950.3 108.0

Annotations:

Y^{1,4}X_{Fuc} Y^{0,3}X_{GlcNAc} ^{0,3}X_{GlcNAc}Y Y^{0,3}X_{Gal} Y^{1,4}X_{Gal} ^{0,3}X_{Gal}Y ^{1,4}X_{Gal}Y ^{0,3}X_{GlcNAc} C^{0,3}X_{NeuAc}
956.4 304.0

Annotations:

YZ ZY YZ ZY
957.8 382.0

Annotations:

Y
965.3 2776.0

Annotations:

CY CY
965.9 945.0

Annotations:

YY YY
966.4 894.0
966.9 369.0

Annotations:

Y^{1,4}X_{GlcNAc}
967.7 297.0

Annotations:

Y^{1,4}X_{GlcNAc}
968.1 281.0
968.9 311.0
970.2 127.0

Annotations:

B^{1,5}X_{Gal} B^{1,5}X_{Gal} ^{0,2}X_{Gal}Z ^{0,4}X_{NeuAc}Z Z^{0,4}X_{NeuAc} Z^{0,2}X_{Gal}
973.3 308.0

Annotations:

Y^{0,4}X_{Fuc}
984.1 128.0
994.3 522.0

Annotations:

ZY YZ
994.8 609.0

Annotations:

Y
996.0 186.0
1001.2 231.0

Annotations:

C^{0,3}X_{Gal} C^{1,4}X_{Gal} ^{1,4}A_{GlcNAc} C^{0,3}X_{Gal} C^{1,4}X_{Gal} Z^{3,5}X_{Fuc} Z^{3,5}X_{Fuc}
1008.9 138.0

Annotations:

C^{3,5}X_{Gal} C^{3,5}X_{Gal} Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} ^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} Y^{1,5}X_{Gal} ^{1,5}X_{Gal}Y ^{1,3}X_{Man} Y^{0,4}X_{Gal} Y^{1,3}X_{Gal} Y^{2,4}X_{Gal} C^{0,3}X_{NeuAc}
1016.4 254.0

Annotations:

^{1,3}X_{NeuAc}Y Y^{1,3}X_{NeuAc} Y^{1,5}X_{Fuc} Y^{0,4}X_{Fuc} Y^{0,4}X_{Fuc}
1017.4 859.0
1018.3 187.0
1019.2 160.0
1020.5 201.0

Annotations:

BY CZ
1022.4 167.0

Annotations:

^{0,3}X_{GlcNAc}Y ^{0,3}X_{GlcNAc}Y Y^{3,5}X_{GlcNAc} ^{3,5}X_{GlcNAc}Y ^{1,5}A_{GlcNAc} Z^{2,5}X_{Fuc}
1028.3 252.0

Annotations:

^{2,4}X_{GlcNAc}Z ^{2,4}X_{GlcNAc}Z
1029.9 412.0

Annotations:

Z Z
1031.5 578.0

Annotations:

Y^{2,5}X_{Fuc}
1035.3 179.0
1036.5 160.0

Annotations:

B
1036.7 259.0

Annotations:

B
1036.8 352.0

Annotations:

B
1037.3 282.0

Annotations:

B
1037.9 243.0

Annotations:

CY
1039.0 747.0

Annotations:

Y Y
1039.3 259.0

Annotations:

Y Y
1039.6 514.0
1039.9 452.0
1040.5 407.0
1040.8 434.0
1041.9 134.0
1049.4 345.0

Annotations:

ZZ ZZ
1049.5 389.0

Annotations:

ZZ ZZ
1049.9 1052.0

Annotations:

YZ ZY
1050.8 300.0

Annotations:

YZ ZY
1051.3 118.0
1053.5 194.0

Annotations:

Y^{0,4}X_{Fuc}
1058.4 328.0

Annotations:

YZ ZY YZ ZY
1058.9 560.0

Annotations:

YY
1059.4 115.0

Annotations:

YY
1060.3 158.0
1069.2 147.0
1072.8 434.0

Annotations:

^{1,5}X_{Gal}Z Z^{1,5}X_{Fuc} ^{1,5}X_{Gal}Z Z^{1,5}X_{Fuc}
1076.3 108.0

Annotations:

Y
1077.0 625.0
1079.6 547.0

Annotations:

^{0,2}X_{Gal}Z Z^{0,2}X_{Fuc} ^{0,2}X_{Gal}Z Z^{0,2}X_{Fuc} ^{0,4}X_{NeuAc}Z ^{2,5}X_{Gal}Z Z^{2,5}X_{Gal}
1087.4 207.0

Annotations:

Z^{2,5}X_{Fuc} ^{2,5}X_{Gal}Z Z^{2,5}X_{Fuc} ^{2,5}X_{Gal}Z
1092.9 110.0
1102.0 605.0

Annotations:

^{3,5}X_{Gal}Z Z^{3,5}X_{Fuc} ^{3,5}X_{Gal}Z Z^{3,5}X_{Fuc}
1102.4 267.0

Annotations:

^{3,5}X_{Gal}Z Z^{3,5}X_{Fuc} ^{3,5}X_{Gal}Z Z^{3,5}X_{Fuc}
1109.4 203.0

Annotations:

^{0,4}X_{Gal}Z ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z Z^{0,4}X_{Fuc} ^{0,4}X_{Gal}Z ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z Z^{0,4}X_{Fuc} ^{1,3}X_{NeuAc}Z Z^{1,3}X_{NeuAc}
1109.9 538.0

Annotations:

^{1,4}A_{Man}Y C^{0,3}X_{Gal} C^{1,4}X_{Gal}
1110.5 1005.0

Annotations:

^{1,4}A_{Man}Y C^{0,3}X_{Gal} C^{1,4}X_{Gal} ^{2,4}X_{GlcNAc} ^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y Y^{0,4}X_{Gal} Y^{1,3}X_{Gal} Y^{2,4}X_{Gal} ^{2,4}X_{GlcNAc} Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} ^{0,2}X_{NeuAc}
1110.8 365.0

Annotations:

^{1,4}A_{Man}Y C^{0,3}X_{Gal} C^{1,4}X_{Gal} ^{2,4}X_{GlcNAc} ^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y Y^{0,4}X_{Gal} Y^{1,3}X_{Gal} Y^{2,4}X_{Gal} Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} ^{0,2}X_{NeuAc} ^{2,4}X_{GlcNAc}
1111.0 586.0

Annotations:

^{0,2}X_{NeuAc} ^{3,5}X_{Gal}Y Y^{3,5}X_{Fuc} ^{3,5}X_{Gal}Y Y^{3,5}X_{Fuc}
1111.5 319.0

Annotations:

^{3,5}X_{Gal}Y Y^{3,5}X_{Fuc} ^{3,5}X_{Gal}Y Y^{3,5}X_{Fuc} ^{1,5}X_{NeuAc}
1111.9 216.0

Annotations:

^{1,5}X_{NeuAc}
1113.5 118.0
1114.3 319.0

Annotations:

YY
1119.0 701.0

Annotations:

^{2,5}X_{NeuAc}
1119.4 335.0
1119.9 246.0
1120.5 189.0
1120.7 245.0
1120.9 143.0
1122.9 410.0

Annotations:

Z^{0,2}X_{Gal}
1123.5 102.0
1131.4 1722.0

Annotations:

Z Z
1132.0 3584.0
1132.5 1061.0
1132.8 614.0
1133.5 135.0

Annotations:

^{3,5}X_{NeuAc}
1134.4 125.0
1139.4 2117.0

Annotations:

Z
1140.0 1705.0

Annotations:

Y Y
1140.5 4739.0

Annotations:

Y Y
1141.0 3568.0
1141.3 1321.0
1141.5 1852.0
1141.8 721.0
1142.0 627.0

Annotations:

Y^{1,5}X_{Fuc}
1142.5 234.0

Annotations:

Y^{1,5}X_{Fuc}
1143.0 183.0
1148.4 695.0

Annotations:

Y
1149.0 997.0
1150.0 239.0
1155.4 259.0

Annotations:

^{0,2}A_{Man}Z
1161.5 722.0

Annotations:

^{0,2}X_{Gal} ^{0,2}X_{Gal} ^{0,4}X_{NeuAc}
1163.0 290.0
1163.5 473.0

Annotations:

^{1,4}X_{NeuAc}
1168.0 741.0

Annotations:

Z^{0,3}X_{Fuc} Z^{3,5}X_{Gal}
1170.5 375.0

Annotations:

^{2,4}X_{NeuAc} Y^{1,4}X_{Fuc} Y^{0,3}X_{Gal} Y^{1,4}X_{Gal}
1173.9 154.0

Annotations:

^{0,2}A_{Man}Y
1182.3 105.0

Annotations:

B
1182.9 736.0

Annotations:

B
1191.0 237.0

Annotations:

^{1,3}X_{Fuc} ^{2,4}X_{Fuc} ^{0,4}X_{Gal} ^{1,3}X_{Gal} ^{2,4}X_{Gal} ^{0,4}X_{Gal} ^{1,3}X_{Gal} ^{2,4}X_{Gal}
1191.5 2578.0

Annotations:

^{1,3}X_{Fuc} ^{2,4}X_{Fuc} ^{0,4}X_{Gal} ^{1,3}X_{Gal} ^{2,4}X_{Gal} ^{0,4}X_{Gal} ^{1,3}X_{Gal} ^{2,4}X_{Gal}
1191.9 2502.0

Annotations:

^{1,3}X_{Fuc} ^{2,4}X_{Fuc} ^{0,4}X_{Gal} ^{1,3}X_{Gal} ^{2,4}X_{Gal} ^{0,4}X_{Gal} ^{1,3}X_{Gal} ^{2,4}X_{Gal}
1192.5 1778.0

Annotations:

^{3,5}X_{Fuc}
1192.9 476.0

Annotations:

^{3,5}X_{Fuc}
1200.5 2170.0

Annotations:

C
1200.9 1797.0

Annotations:

C
1201.5 1278.0
1202.0 368.0

Annotations:

Y^{3,5}X_{Fuc}
1203.0 182.0
1203.2 270.0
1203.5 267.0
1203.9 364.0
1212.0 520.0
1212.5 1980.0
1212.9 513.0
1213.5 1224.0
1213.9 196.0
1214.3 372.0
1218.5 110.0
1220.5 1054.0
1220.7 191.0
1220.9 169.0
1221.5 1775.0

Annotations:

1242.3 108.0

Annotations:

Z
1245.1 123.0
1245.5 104.0
1260.7 180.0

Annotations:

Y
1262.6 258.0
1344.6 411.0

Annotations:

BY CZ
1345.0 170.0
1345.5 172.0
1346.5 408.0

Annotations:

^{1,4}A_{GlcNAc}Y C^{0,3}X_{GlcNAc}
1349.5 145.0
1386.9 112.0

Annotations:

ZZ ZZ
1392.6 161.0
1447.4 282.0
1478.4 152.0

Annotations:

^{2,4}A_{GlcNAc}Y ^{1,5}A_{Man}Y ^{1,5}A_{Man}Y
1493.6 517.0
1526.6 251.0
1583.8 121.0
1590.7 173.0
1591.8 366.0
1630.0 133.0
1643.8 263.0
1709.5 511.0

Annotations:

CZ BY BY CZ BY BY CZ CZ
1710.6 321.0
1711.7 221.0

Annotations:

^{1,4}A_{GlcNAc}Y ^{2,5}X_{NeuAc}Y Z^{3,5}X_{Fuc}
1728.6 190.0
1747.5 169.0
1751.5 141.0

Annotations:

ZZ ZZ
1767.0 373.0

Annotations:

^{2,4}X_{GlcNAc}Z
1787.6 231.0

Annotations:

Y
1788.8 338.0

Annotations:

C^{2,4}X_{NeuAc}
1829.8 103.0

Annotations:

C^{0,4}X_{Gal} C^{1,3}X_{Gal} C^{2,4}X_{Gal} ^{0,2}A_{GlcNAc}Y ^{0,2}A_{GlcNAc}Y C^{0,4}X_{Gal} C^{1,3}X_{Gal} C^{2,4}X_{Gal} ^{0,2}A_{Man}
1844.7 227.0

Annotations:

YY
1871.6 330.0

Annotations:

B
1872.8 331.0

Annotations:

^{1,3}X_{Man}Y Y^{0,4}X_{Fuc} ^{1,3}X_{NeuAc}Y Y^{1,5}X_{Fuc}
1873.5 222.0

Annotations:

C^{1,5}X_{NeuAc} ^{2,5}X_{NeuAc}Y Y^{2,5}X_{NeuAc}
1889.7 1060.0

Annotations:

C
1890.0 571.0

Annotations:

C
1890.7 1167.0
1890.9 897.0
1891.8 264.0
1895.6 169.0
1912.7 419.0

Annotations:

BY BY CZ CZ
1914.0 231.0
1914.3 337.0

Annotations:

YZ ZY YZ ZY
1917.0 183.0

Annotations:

Y
1932.6 260.0

Annotations:

YY YY
1932.8 270.0

Annotations:

YY YY
1942.7 185.0

Annotations:

^{1,5}X_{GlcNAc}Z ^{1,5}X_{GlcNAc}Z Z^{1,5}X_{Fuc} Z^{1,5}X_{Fuc}
1973.8 537.0

Annotations:

B^{2,4}X_{NeuAc}
1974.8 165.0

Annotations:

Y^{0,2}X_{Fuc} Y^{0,2}X_{Fuc} ^{2,5}X_{Gal}Y Y^{2,5}X_{Gal}
1990.9 189.0

Annotations:

Y
1991.1 323.0

Annotations:

Y
1991.9 321.0

Annotations:

C^{2,4}X_{NeuAc} ^{0,2}A_{GlcNAc}
1992.1 194.0

Annotations:

C^{2,4}X_{NeuAc} ^{0,2}A_{GlcNAc}
1992.9 187.0
2015.7 220.0

Annotations:

^{0,2}X_{GlcNAc}Y ^{0,2}X_{GlcNAc}Y Y^{2,5}X_{GlcNAc} ^{2,5}X_{GlcNAc}Y
2020.7 275.0

Annotations:

Y^{3,5}X_{Fuc} Y^{3,5}X_{Fuc}
2021.9 188.0
2037.9 150.0
2058.6 194.0

Annotations:

Z^{2,5}X_{NeuAc} ^{0,2}X_{NeuAc}Z ^{2,5}X_{NeuAc}Z
2061.7 276.0

Annotations:

^{3,5}X_{GlcNAc}Y ^{3,5}X_{GlcNAc}Y
2063.6 143.0
2074.8 433.0

Annotations:

B
2075.8 192.0

Annotations:

^{2,4}X_{GlcNAc}Y ^{2,4}X_{GlcNAc}Y
2076.0 354.0

Annotations:

^{2,4}X_{GlcNAc}Y ^{2,4}X_{GlcNAc}Y
2076.6 385.0

Annotations:

^{0,2}X_{NeuAc}Y ^{2,5}X_{NeuAc}Y Y^{2,5}X_{NeuAc} Z^{3,5}X_{Fuc}
2078.8 112.0

Annotations:

Y Y
2080.0 213.0
2092.8 291.0

Annotations:

C
2093.1 261.0

Annotations:

C
2093.6 172.0
2094.9 413.0

Annotations:

Y^{3,5}X_{Fuc}
2110.9 164.0
2136.0 187.0

Annotations:

YY YY
2139.0 388.0
2142.8 354.0
2151.7 157.0
2152.5 189.0

Annotations:

Y
2152.8 483.0

Annotations:

Y
2153.8 987.0
2154.1 216.0
2154.8 376.0
2155.3 171.0
2156.8 492.0

There are 7 similar spectra:

Id Structure Score

2588

1.00

2414

0.79

2412

0.77

2587

0.77

2566

0.77

2253

0.72

2413

0.57
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