In-depth N-glycome profiling of paired colorectal cancer and non-tumorigenic tissues reveals cancer-, stage- and EGFR-specific protein N-glycosylation.

PubMed Entry

GlyToucan Accession Number: G46031NV

Peak Intensity
350.1 186.0

Annotations:

Z
350.3 109.0

Annotations:

Z
351.4 101.0

Annotations:

B^{2,4}X_{NeuAc} ^{3,5}X_{GlcNAc}Y
368.1 175.0

Annotations:

Y
382.0 147.0

Annotations:

C
407.3 104.0

Annotations:

YZ ZY
424.1 382.0

Annotations:

C^{0,2}X_{Gal} C^{0,2}X_{Gal} ^{1,5}A_{Gal} ^{1,3}A_{Man} ^{1,3}A_{Man}Y ^{0,4}A_{Man}Y ^{1,3}A_{Man}Y ^{2,4}A_{Man}Y
554.1 170.0

Annotations:

^{1,5}X_{Gal}Z Z^{1,5}X_{Man} ^{1,5}X_{Man}Z Z^{1,5}X_{Gal} B^{2,4}X_{NeuAc} ^{3,5}A_{GlcNAc}Z
571.2 224.0

Annotations:

Y
586.3 123.0

Annotations:

^{0,2}A_{Man}Y ^{0,4}A_{Man} ^{1,3}A_{Man}Y ^{2,4}A_{Man}Y ^{0,2}A_{GlcNAc}Y C^{0,4}X_{Gal}
621.1 139.0

Annotations:

Z
655.2 646.0

Annotations:

B
656.2 223.0

Annotations:

^{0,2}X_{NeuAc}Z
670.0 108.0

Annotations:

BZ
672.3 154.0

Annotations:

^{1,4}A_{GlcNAc}Y C^{0,3}X_{Gal} ^{2,5}X_{NeuAc}Y Z^{3,5}X_{Fuc}
688.1 200.0

Annotations:

BY CZ
692.3 233.0

Annotations:

ZZ
692.7 255.0

Annotations:

ZZ
701.9 441.0

Annotations:

Z
702.3 137.0

Annotations:

YY
708.7 147.0

Annotations:

BY CZ
731.4 132.0

Annotations:

YY
738.0 130.0

Annotations:

^{1,5}X_{NeuAc}Z Z^{1,4}X_{GlcNAc} ^{1,4}X_{Man}Y Y^{0,3}X_{Fuc}
753.3 166.0

Annotations:

^{1,5}X_{Gal}Y ^{1,3}X_{Man}Y Y^{0,4}X_{Fuc} ^{1,3}X_{NeuAc}Y Y^{1,5}X_{Fuc} C^{1,5}X_{NeuAc} ^{2,5}X_{NeuAc}Y
753.8 112.0

Annotations:

C^{1,5}X_{NeuAc} ^{2,5}X_{NeuAc}Y
774.8 135.0

Annotations:

Y
775.3 107.0

Annotations:

Y
782.8 2478.0

Annotations:

YY
783.3 1045.0

Annotations:

YY
783.7 966.0

Annotations:

YY
786.3 185.0
786.6 124.0
797.4 136.0

Annotations:

Y^{1,5}X_{Fuc} ^{1,5}X_{GlcNAc}Y ^{0,3}A_{GlcNAc}
799.4 143.0
804.0 147.0

Annotations:

B^{2,4}X_{NeuAc} ^{1,5}A_{Man}Z Y^{0,2}X_{Fuc}
813.4 100.0
817.3 879.0

Annotations:

B
818.4 519.0

Annotations:

^{1,4}A_{GlcNAc} C^{0,3}X_{Gal} C^{1,4}X_{Gal} Z^{3,5}X_{Fuc}
819.3 151.0

Annotations:

^{1,4}A_{GlcNAc}Y ^{1,4}X_{GlcNAc}Y Y^{0,3}X_{Fuc}
826.5 160.0

Annotations:

C^{3,5}X_{Gal} Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} Y^{1,5}X_{Gal} ^{1,5}X_{Gal} ^{1,3}X_{Man}
831.3 176.0
835.3 1848.0

Annotations:

C
836.2 678.0
837.1 218.0
837.4 221.0
838.1 114.0

Annotations:

^{0,2}X_{NeuAc}Z
845.8 444.0

Annotations:

^{2,4}X_{GlcNAc}Z ^{1,5}A_{GlcNAc}Z ^{0,2}X_{NeuAc}Z ^{2,5}X_{NeuAc}Z
846.4 313.0

Annotations:

^{0,2}X_{NeuAc}Z ^{2,5}X_{NeuAc}Z
847.3 236.0

Annotations:

Z
851.8 206.0
855.4 318.0

Annotations:

^{0,2}X_{NeuAc}Y ^{2,5}X_{NeuAc}Y Z^{3,5}X_{Fuc}
855.8 139.0

Annotations:

^{1,5}X_{NeuAc}Y
856.3 268.0

Annotations:

Y
856.7 171.0

Annotations:

Y
856.9 266.0
859.4 121.0

Annotations:

ZZ ZZ
867.3 314.0

Annotations:

ZZ
868.3 140.0

Annotations:

CY
875.9 805.0

Annotations:

YZ ZY
876.2 174.0

Annotations:

YZ ZY
876.8 338.0

Annotations:

^{1,3}A_{Man} ^{2,4}A_{Man}
877.3 125.0

Annotations:

YZ ZY YZ ZY
878.4 168.0

Annotations:

B^{1,5}X_{GlcNAc} ^{3,5}X_{NeuAc}Y
885.9 248.0
891.3 227.0

Annotations:

BY CZ
895.3 184.0

Annotations:

YY YY
901.2 168.0
905.8 373.0

Annotations:

^{1,5}X_{GlcNAc}Z Z^{1,5}X_{Man} ^{1,5}X_{Man}Z Z^{1,5}X_{GlcNAc} ^{0,2}X_{Gal}Y Y^{0,2}X_{Fuc} ^{0,4}X_{NeuAc}Y ^{2,5}X_{GlcNAc}
908.9 170.0

Annotations:

CY
911.2 109.0
915.3 283.0

Annotations:

^{2,4}X_{NeuAc}Y
926.9 358.0

Annotations:

^{1,3}X_{NeuAc}Z ^{0,4}X_{Gal}Z ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z Z^{0,4}X_{Fuc}
927.5 485.0

Annotations:

Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} ^{2,4}X_{GlcNAc}
927.9 232.0

Annotations:

Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} ^{2,4}X_{GlcNAc} ^{0,2}X_{NeuAc}
928.3 465.0

Annotations:

Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} ^{2,4}X_{GlcNAc} ^{0,2}X_{NeuAc}
929.5 137.0

Annotations:

^{1,5}X_{NeuAc}
936.4 618.0

Annotations:

^{2,5}A_{GlcNAc}Z ^{2,5}X_{NeuAc}
936.9 410.0

Annotations:

C^{1,5}X_{Man}
937.2 150.0

Annotations:

C^{1,5}X_{Man}
937.5 289.0

Annotations:

C^{1,5}X_{Man}
939.6 179.0
948.8 844.0

Annotations:

Z
949.4 839.0

Annotations:

B^{2,5}X_{GlcNAc} ^{1,4}X_{GlcNAc}Z Z^{1,4}X_{Man} ^{1,4}X_{Man}Z Z^{1,4}X_{GlcNAc}
949.7 149.0

Annotations:

B^{2,5}X_{GlcNAc} ^{1,4}X_{GlcNAc}Z Z^{1,4}X_{Man} ^{1,4}X_{Man}Z Z^{1,4}X_{GlcNAc}
950.2 151.0
956.4 137.0

Annotations:

Z
956.9 2057.0

Annotations:

Z
957.4 2614.0

Annotations:

Y
957.8 1355.0

Annotations:

Y
958.4 695.0
958.9 692.0
965.9 110.0

Annotations:

Y
967.0 128.0

Annotations:

C^{2,5}X_{GlcNAc} ^{1,4}X_{GlcNAc}Y Y^{1,4}X_{Man} ^{1,4}X_{Man}Y Y^{1,4}X_{GlcNAc}
972.3 112.0

Annotations:

^{1,5}X_{Gal}
972.8 201.0
978.5 284.0

Annotations:

^{0,2}X_{GlcNAc}Y Y^{0,2}X_{GlcNAc} ^{0,2}X_{Gal} ^{0,4}X_{NeuAc}
978.8 194.0

Annotations:

^{0,2}X_{GlcNAc}Y Y^{0,2}X_{GlcNAc} ^{0,2}X_{Gal} ^{0,4}X_{NeuAc}
979.4 300.0

Annotations:

BY CZ
988.4 148.0

Annotations:

^{2,4}X_{NeuAc}
988.7 105.0

Annotations:

^{2,4}X_{NeuAc}
989.3 211.0
997.4 198.0

Annotations:

CY
1001.3 410.0
1003.8 126.0
1004.4 126.0
1006.2 105.0

Annotations:

^{3,5}X_{GlcNAc}Z Z^{3,5}X_{GlcNAc}
1008.9 1739.0

Annotations:

^{1,3}X_{Fuc} ^{2,4}X_{Fuc} ^{0,4}X_{Gal} ^{1,3}X_{Gal} ^{2,4}X_{Gal}
1009.3 930.0

Annotations:

^{1,3}X_{Fuc} ^{2,4}X_{Fuc} ^{0,4}X_{Gal} ^{1,3}X_{Gal} ^{2,4}X_{Gal}
1009.9 646.0

Annotations:

^{3,5}X_{Fuc}
1010.4 125.0
1010.7 140.0
1017.9 1933.0
1018.3 952.0
1018.9 988.0
1019.4 438.0
1019.8 165.0
1020.3 123.0

Annotations:

^{2,4}X_{GlcNAc}Z Z^{2,4}X_{GlcNAc}
1021.2 407.0

Annotations:

ZZ
1028.9 486.0
1029.3 355.0
1030.1 149.0
1030.4 475.0
1032.0 107.0
1057.3 100.0

Annotations:

YY
1095.4 207.0

Annotations:

^{2,4}A_{GlcNAc}Z B^{0,2}X_{Gal} ^{1,5}A_{Man}Z
1097.5 110.0
1100.4 147.0
1161.3 106.0
1201.6 140.0

Annotations:

^{2,4}A_{GlcNAc}Y
1202.4 109.0

Annotations:

Y^{3,5}X_{Fuc}
1218.7 107.0
1225.6 247.0
1260.6 129.0

Annotations:

Y
1276.5 189.0

Annotations:

YY
1362.5 292.0

Annotations:

CY CY
1363.5 129.0
1380.5 149.0
1380.8 125.0
1384.6 116.0
1404.6 127.0

Annotations:

Z
1418.5 110.0

Annotations:

BY CZ
1422.6 103.0

Annotations:

Y
1425.9 123.0
1445.8 118.0

Annotations:

YZ ZY
1449.4 213.0
1461.6 110.0

Annotations:

YZ ZY
1465.6 236.0
1469.2 201.0
1505.6 125.0

Annotations:

C^{2,4}X_{GlcNAc} Y^{0,2}X_{GlcNAc} Y^{0,2}X_{Gal} ^{0,2}X_{GlcNAc} ^{0,2}X_{Gal}Y
1505.7 104.0

Annotations:

C^{2,4}X_{GlcNAc} Y^{0,2}X_{GlcNAc} Y^{0,2}X_{Gal} ^{0,2}X_{GlcNAc} ^{0,2}X_{Gal}Y
1506.5 309.0

Annotations:

B
1507.5 137.0

Annotations:

^{1,5}X_{Gal}Y Y^{0,4}X_{Fuc} ^{1,3}X_{NeuAc}Y Y^{1,5}X_{Fuc} ^{1,3}X_{Man}Y
1507.6 139.0

Annotations:

^{1,5}X_{Gal}Y ^{1,3}X_{Man}Y Y^{0,4}X_{Fuc} ^{1,3}X_{NeuAc}Y Y^{1,5}X_{Fuc}
1508.5 106.0

Annotations:

C^{1,5}X_{NeuAc} ^{2,5}X_{NeuAc}Y
1524.7 659.0

Annotations:

C
1525.6 825.0
1526.7 182.0
1527.7 129.0
1529.6 122.0

Annotations:

BZ
1533.6 107.0

Annotations:

Z
1547.5 259.0

Annotations:

BY CZ
1548.5 274.0
1549.6 272.0

Annotations:

YZ ZY
1551.2 105.0

Annotations:

Y
1566.5 157.0

Annotations:

^{2,4}A_{GlcNAc}
1567.6 109.0

Annotations:

YY
1607.5 222.0

Annotations:

Z
1610.6 257.0

Annotations:

^{2,5}A_{GlcNAc}
1612.9 130.0

Annotations:

C^{1,4}X_{NeuAc} ^{2,4}X_{NeuAc}Y
1624.7 122.0
1625.7 187.0

Annotations:

Y
1626.5 391.0

Annotations:

C^{2,4}X_{NeuAc} ^{0,2}A_{GlcNAc}
1626.7 368.0

Annotations:

C^{2,4}X_{NeuAc} ^{0,2}A_{GlcNAc}
1627.1 212.0
1627.7 185.0
1628.7 126.0
1654.7 154.0
1670.6 125.0
1691.7 148.0
1693.7 334.0

Annotations:

^{0,2}X_{NeuAc}Z ^{2,5}X_{NeuAc}Z
1709.7 881.0

Annotations:

B
1710.7 574.0

Annotations:

^{2,4}X_{GlcNAc}Y
1711.6 439.0

Annotations:

^{0,2}X_{NeuAc}Y ^{2,5}X_{NeuAc}Y Z^{3,5}X_{Fuc}
1727.7 608.0

Annotations:

C
1728.7 264.0
1729.6 230.0

Annotations:

Y^{3,5}X_{Fuc}
1735.6 218.0
1787.5 310.0

Annotations:

Y
1788.1 228.0

Annotations:

Y
1788.8 441.0
1789.7 156.0
1789.9 149.0
1857.7 102.0

Annotations:

^{0,2}X_{NeuAc}
1900.6 111.0

There are 6 similar spectra:

Id Structure Score

2246

0.86

2540

0.75

2367

0.72

2362

0.68

2364

0.57

2245

0.51
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