Cystic fibrosis and bacterial colonization define the sputum N-glycosylation phenotype

PubMed Entry

GlyToucan Accession Number: G45176DV

Peak Intensity
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C
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B
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C^{3,5}X_{NeuAc}
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BZ BZ BZ
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BY CZ
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B^{2,4}X_{NeuAc} ^{3,5}X_{GlcNAc}
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B^{2,4}X_{NeuAc}
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BY BY CZ CZ BY CZ
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YZ ZY YZ ZY
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CY
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Z
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B
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Y
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Y
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B
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C
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C
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B
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B
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B^{1,5}X_{GlcNAc} ^{0,3}X_{Gal}Y Y^{1,4}X_{Man} ^{0,3}X_{GlcNAc}Y
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C
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C
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CY
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C^{2,5}X_{Fuc}
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CY CY
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CY CY
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Z
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^{1,5}A_{GlcNAc}Z B^{2,5}X_{GlcNAc}
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C^{1,3}X_{Man} Y^{0,2}X_{Gal} ^{2,5}A_{GlcNAc}Y ^{2,5}A_{Man}Y
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B
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C^{3,5}X_{NeuAc}
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BZ BY CZ
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B
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B
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C
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C
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Z
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^{0,3}X_{GlcNAc}Y ^{3,5}X_{GlcNAc}Y
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Z
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Z Y
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Y
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^{1,4}X_{NeuAc}Z ^{3,5}A_{GlcNAc}Y
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ZZ
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^{0,2}A_{Man}Z B^{2,5}X_{NeuAc} ^{2,5}A_{GlcNAc}Z ^{0,4}X_{NeuAc}Y
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^{1,4}X_{NeuAc}Y
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C^{2,5}X_{NeuAc} ^{2,5}A_{GlcNAc}Y ^{0,4}A_{Man} ^{2,4}X_{NeuAc}Y
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C^{2,5}X_{NeuAc} ^{2,5}A_{GlcNAc}Y ^{0,4}A_{Man} ^{2,4}X_{NeuAc}Y
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^{2,4}X_{NeuAc}Y
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C^{2,4}X_{NeuAc}
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YY
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CZ BY
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YY YY
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CZ BY
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CY
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CY
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B^{1,5}X_{Gal} ^{1,5}A_{GlcNAc}Z ^{1,5}X_{GlcNAc}Z Z^{1,5}X_{Man} ^{1,5}X_{Man}Z ^{2,5}X_{Gal}Z Z^{2,5}X_{Fuc}
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CY
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Annotations:

Z
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^{2,4}A_{GlcNAc}Y C^{0,2}X_{Gal} C^{0,4}X_{NeuAc} B^{2,4}X_{NeuAc} ^{0,3}X_{Gal}Y ^{3,5}X_{Gal}Y Y^{0,3}X_{Fuc} Y^{3,5}X_{Gal} ^{3,5}X_{GlcNAc}
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Annotations:

B^{2,4}X_{NeuAc} Y^{3,5}X_{Gal} ^{3,5}X_{GlcNAc} C^{1,5}X_{Fuc} ^{0,3}X_{Gal}Y ^{3,5}X_{Gal}Y Y^{0,3}X_{Fuc}
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Annotations:

C^{1,5}X_{Fuc}
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C^{1,4}X_{NeuAc} ^{1,5}X_{GlcNAc}Y ^{1,5}X_{Man}Y Y^{1,5}X_{Man}
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Annotations:

C^{1,4}X_{NeuAc} ^{1,5}X_{GlcNAc}Y ^{1,5}X_{Man}Y Y^{1,5}X_{Man}
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Annotations:

^{0,3}A_{GlcNAc}Z ^{3,5}A_{GlcNAc}Z B^{1,4}X_{Fuc} B^{0,3}X_{Gal} B^{1,4}X_{Gal}
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Y
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Y
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C^{2,4}X_{NeuAc}
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C^{2,4}X_{NeuAc}
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Annotations:

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Annotations:

^{0,3}A_{GlcNAc}Y C^{0,3}X_{Fuc} C^{3,5}X_{Gal}
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ZZ
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ZZ
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ZZ
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^{0,2}A_{GlcNAc}Z B^{0,4}X_{Fuc} B^{1,3}X_{NeuAc}
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^{2,5}A_{GlcNAc}Y C^{0,4}X_{Gal} C^{1,3}X_{Gal} C^{2,4}X_{Gal} ^{0,2}A_{GlcNAc}Y C^{1,3}X_{Fuc} C^{2,4}X_{Fuc} ^{0,2}A_{Man}
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^{1,4}A_{GlcNAc}Y
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YZ ZY
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YZ ZY
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BZ
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B
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^{1,4}A_{GlcNAc}Y
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YY
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B^{0,3}X_{NeuAc} ^{1,5}A_{GlcNAc}Z
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^{0,2}X_{GlcNAc}Y
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C
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C C
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C
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BZ
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BZ
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CZ BY
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CZ BY
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CZ BY
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Annotations:

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Annotations:

^{1,5}X_{NeuAc}Y Y^{1,4}X_{Fuc} Y^{0,3}X_{Gal} Y^{1,4}X_{Gal} ^{0,3}X_{Gal} ^{2,5}X_{GlcNAc}Y C^{3,5}X_{NeuAc}
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CY
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Annotations:

CY
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^{2,4}A_{GlcNAc} ^{1,5}X_{NeuAc}Y
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^{1,5}X_{NeuAc}Y Y^{0,3}X_{Fuc} Y^{3,5}X_{Gal} ^{3,5}X_{Gal}
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Annotations:

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Annotations:

ZZ
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ZZ
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ZZ
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B^{1,5}X_{Fuc}
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C^{1,5}X_{Gal} ^{3,5}X_{NeuAc}Z
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Annotations:

^{0,3}X_{NeuAc}Y ^{3,5}X_{NeuAc}Y ^{0,3}A_{GlcNAc}
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^{2,4}X_{GlcNAc}Z
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Annotations:

C^{0,2}X_{Gal} C^{0,4}X_{NeuAc} B^{2,4}X_{NeuAc} C^{1,5}X_{Fuc} ^{1,3}A_{Man} ^{2,4}A_{Man}
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Annotations:

C^{1,5}X_{Fuc} ^{1,3}A_{Man} ^{2,4}A_{Man} ^{2,5}A_{GlcNAc}
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Annotations:

Z
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Annotations:

B^{0,4}X_{Gal} B^{1,3}X_{Gal} B^{2,4}X_{Gal} B^{1,3}X_{Fuc} B^{2,4}X_{Fuc} ^{0,4}X_{NeuAc}Y
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Annotations:

^{0,4}X_{NeuAc}Y B^{3,5}X_{Fuc} ^{2,4}X_{NeuAc}Z
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Annotations:

^{0,4}X_{NeuAc}Y B^{3,5}X_{Fuc} ^{2,4}X_{NeuAc}Z
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Annotations:

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Annotations:

C^{3,5}X_{Fuc} ^{1,4}A_{Man} ^{2,4}X_{NeuAc}Y
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Annotations:

C^{3,5}X_{Fuc} ^{1,4}A_{Man} ^{2,4}X_{NeuAc}Y
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Annotations:

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Annotations:

YY
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Annotations:

YY
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Annotations:

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^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z
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Annotations:

^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z
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Annotations:

Z^{3,5}X_{Gal} ^{3,5}X_{Fuc}Z
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B
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B
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Annotations:

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Annotations:

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^{1,5}X_{NeuAc}
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Annotations:

ZY YZ
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Annotations:

^{2,5}A_{GlcNAc}Y ^{2,5}A_{Man}Y
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Annotations:

^{2,5}A_{GlcNAc}Y ^{2,5}A_{Man}Y
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Annotations:

^{1,3}X_{NeuAc}Y
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Annotations:

Z
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Annotations:

Y
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Annotations:

Y
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Annotations:

BZ
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Annotations:

^{1,4}X_{GlcNAc}Z ^{1,4}A_{GlcNAc}Y
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Annotations:

Y
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Annotations:

^{0,2}X_{Gal}Z ^{0,2}X_{GlcNAc}Z
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Annotations:

BZ
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Annotations:

BY CZ
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Annotations:

BY CZ
980.3 280.0

Annotations:

^{1,5}X_{Fuc} ^{1,5}X_{Gal}Y Y^{1,5}X_{Man}
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Annotations:

^{1,5}X_{Gal}Y Y^{1,5}X_{Man}
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Annotations:

^{0,2}X_{Fuc} ^{2,5}X_{Gal}
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Annotations:

^{2,4}X_{NeuAc}
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Annotations:

^{2,4}X_{NeuAc}
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Annotations:

^{2,5}X_{Gal}Z ^{2,5}X_{GlcNAc}Z
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Annotations:

^{0,2}X_{GlcNAc}Y ^{1,4}X_{Fuc} ^{0,3}X_{Gal} ^{1,4}X_{Gal} ^{0,2}X_{Gal}Y
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Annotations:

CY
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Annotations:

^{0,3}X_{Gal}Z Z^{1,4}X_{Man} ^{0,3}X_{GlcNAc}Z
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Annotations:

B^{1,5}X_{Man}
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1008.4 131.0

Annotations:

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Annotations:

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Annotations:

^{1,5}A_{GlcNAc}Y ^{3,5}X_{Fuc}
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Annotations:

^{3,5}X_{Fuc} ^{2,5}X_{Gal}Y ^{2,5}X_{GlcNAc}Y
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Annotations:

^{2,5}X_{Gal}Y ^{2,5}X_{GlcNAc}Y
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Annotations:

^{0,4}X_{Fuc} ^{1,3}X_{NeuAc}
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Annotations:

BZ
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Annotations:

BZ
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1022.3 244.0

Annotations:

^{3,5}X_{Gal}Z ^{3,5}X_{GlcNAc}Z
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Annotations:

ZZ ZZ ZZ
1082.6 295.0

Annotations:

^{2,5}A_{GlcNAc}Z
1099.5 152.0

Annotations:

^{0,2}A_{GlcNAc}Y C^{1,3}X_{Man} ^{0,2}A_{Man}Y
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Annotations:

CY
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Annotations:

CY
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1174.4 367.0
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Annotations:

BY CZ
1183.5 197.0

Annotations:

^{2,4}A_{GlcNAc}Z
1186.4 151.0

Annotations:

^{1,5}X_{NeuAc}Y Y^{1,4}X_{Man} ^{0,3}X_{Gal}Y ^{1,4}X_{Man}Y Y^{0,3}X_{Fuc} Y^{3,5}X_{Gal}
1196.4 189.0

Annotations:

^{0,3}X_{NeuAc}Z
1201.5 820.0

Annotations:

^{2,4}A_{GlcNAc}Y
1202.4 296.0

Annotations:

^{3,5}X_{Gal}Y Y^{3,5}X_{Fuc}
1203.6 284.0
1205.8 112.0
1216.3 390.0

Annotations:

^{1,3}X_{Man}Y Y^{1,3}X_{Man} ^{0,4}X_{Gal}Y Y^{0,4}X_{Fuc}
1218.5 246.0
1219.5 231.0
1224.5 253.0

Annotations:

ZZ
1243.4 328.0

Annotations:

^{0,2}A_{GlcNAc}Z
1243.8 239.0

Annotations:

^{0,2}A_{GlcNAc}Z
1244.6 516.0
1255.5 123.0

Annotations:

^{3,5}A_{GlcNAc}Z
1256.8 164.0
1258.8 107.0

Annotations:

YZ ZY YZ ZY
1260.5 359.0

Annotations:

Y
1261.3 155.0

Annotations:

^{0,2}A_{GlcNAc}Y
1261.6 103.0

Annotations:

^{0,2}A_{GlcNAc}Y
1262.1 121.0
1262.6 334.0
1263.7 113.0
1276.3 529.0

Annotations:

YY YY
1276.5 216.0

Annotations:

YY YY
1284.4 418.0

Annotations:

B^{1,3}X_{Man} Z^{0,2}X_{Gal}
1285.8 137.0

Annotations:

^{2,5}A_{GlcNAc}Z ^{2,5}A_{Man}Z ^{0,4}X_{NeuAc}Y
1286.5 231.0

Annotations:

^{1,5}X_{GlcNAc}Z Z^{1,5}X_{Fuc} ^{2,4}X_{NeuAc}Z
1295.6 130.0
1309.7 213.0
1326.5 298.0

Annotations:

BZ
1327.4 120.0
1339.4 145.0
1340.3 186.0
1344.4 322.0

Annotations:

BY CZ
1344.6 276.0

Annotations:

BY CZ
1345.5 865.0
1346.7 203.0

Annotations:

C^{3,5}X_{GlcNAc} ^{0,2}X_{NeuAc}Y Z^{3,5}X_{Fuc}
1347.4 125.0
1357.6 245.0

Annotations:

^{2,5}X_{GlcNAc}Z
1362.5 660.0

Annotations:

CY
1363.5 242.0
1364.3 152.0

Annotations:

Y^{3,5}X_{Fuc}
1365.8 150.0
1368.5 3759.0
1369.5 1834.0
1370.4 666.0
1370.5 926.0
1371.5 400.0

Annotations:

^{0,3}X_{GlcNAc}Z ^{3,5}X_{GlcNAc}Z
1372.5 137.0
1373.8 101.0

Annotations:

^{0,3}X_{GlcNAc}Y
1380.6 255.0
1381.6 109.0
1382.3 148.0
1384.6 227.0

Annotations:

BZ
1386.5 146.0
1387.5 192.0

Annotations:

Z
1388.3 413.0
1404.6 456.0

Annotations:

Z
1405.3 116.0

Annotations:

Y
1406.3 144.0
1406.6 127.0
1406.8 172.0
1410.9 179.0
1411.4 168.0
1421.6 125.0

Annotations:

^{2,4}A_{GlcNAc}Y
1422.6 2533.0

Annotations:

Y
1423.6 1260.0
1424.0 102.0
1424.5 329.0
1425.8 181.0
1427.7 130.0

Annotations:

ZZ
1445.7 195.0

Annotations:

YZ ZY
1446.5 104.0

Annotations:

^{0,2}A_{Man}Z
1448.6 159.0

Annotations:

^{2,5}A_{Man}Y C^{1,5}X_{Gal} ^{0,2}A_{Man}Y ^{2,4}X_{NeuAc}Z
1466.6 264.0

Annotations:

^{2,4}X_{NeuAc}Y
1467.6 383.0
1487.6 156.0

Annotations:

^{0,2}X_{Gal}Z Z^{0,2}X_{Gal}
1492.7 189.0

Annotations:

C^{0,3}X_{Gal} ^{1,4}A_{GlcNAc}Y
1493.6 120.0
1499.7 459.0
1507.5 477.0

Annotations:

^{1,5}X_{Gal}Y Y^{1,5}X_{Fuc} ^{1,3}X_{Man}
1507.8 384.0

Annotations:

^{1,5}X_{Gal}Y Y^{1,5}X_{Fuc} ^{1,3}X_{Man}
1508.4 166.0

Annotations:

CY
1508.7 511.0

Annotations:

CY
1509.0 498.0

Annotations:

CY
1509.4 286.0
1509.6 380.0
1510.5 223.0
1511.0 101.0
1511.6 329.0
1512.7 411.0
1513.5 205.0
1514.3 127.0
1517.6 282.0

Annotations:

^{0,3}X_{Gal}Z Z^{1,4}X_{Fuc} Z^{0,3}X_{Gal} Z^{1,4}X_{Gal}
1525.4 243.0
1525.8 147.0
1526.7 372.0
1529.4 173.0
1531.4 168.0
1531.7 415.0
1533.4 168.0

Annotations:

^{0,3}X_{Gal}Z ^{3,5}X_{Gal}Z Z^{0,3}X_{Fuc} Z^{3,5}X_{Gal}
1534.6 126.0

Annotations:

B^{1,5}X_{Fuc}
1546.8 133.0

Annotations:

BZ
1548.5 477.0

Annotations:

^{2,4}A_{GlcNAc}Z B^{0,2}X_{Fuc} B^{2,5}X_{Gal}
1549.4 1122.0

Annotations:

Z
1549.6 1028.0

Annotations:

Z
1550.7 579.0

Annotations:

^{2,4}A_{GlcNAc}Y C^{0,2}X_{Gal} C^{0,4}X_{NeuAc}
1551.6 494.0

Annotations:

B^{2,4}X_{NeuAc} ^{0,3}X_{Gal}Y ^{3,5}X_{Gal}Y Y^{0,3}X_{Fuc} Y^{3,5}X_{Gal} ^{3,5}X_{GlcNAc}
1551.7 409.0

Annotations:

B^{2,4}X_{NeuAc} ^{0,3}X_{Gal}Y ^{3,5}X_{Gal}Y Y^{0,3}X_{Fuc} Y^{3,5}X_{Gal} ^{3,5}X_{GlcNAc}
1559.6 152.0
1560.6 620.0
1561.0 409.0
1566.5 190.0

Annotations:

^{2,4}A_{GlcNAc}Y C^{0,2}X_{Fuc} C^{2,5}X_{Gal}
1566.6 180.0

Annotations:

^{2,4}A_{GlcNAc}Y C^{0,2}X_{Fuc} C^{2,5}X_{Gal}
1567.6 1566.0

Annotations:

Y
1568.7 1146.0
1569.6 1546.0

Annotations:

C^{2,4}X_{NeuAc}
1570.3 140.0
1570.6 347.0
1571.5 247.0
1589.7 646.0

Annotations:

ZZ
1590.5 296.0
1591.4 154.0
1591.7 134.0
1592.6 364.0

Annotations:

^{0,2}A_{GlcNAc}Z ^{2,5}A_{GlcNAc}Z B^{0,4}X_{Gal} B^{1,3}X_{Gal} B^{2,4}X_{Gal} B^{1,3}X_{Fuc} B^{2,4}X_{Fuc}
1595.7 171.0
1608.7 116.0

Annotations:

^{0,2}A_{GlcNAc}Z B^{0,4}X_{Fuc} B^{1,3}X_{NeuAc}
1610.6 252.0

Annotations:

^{2,5}A_{GlcNAc}Y C^{0,4}X_{Gal} C^{1,3}X_{Gal} C^{2,4}X_{Gal} ^{0,2}A_{GlcNAc}Y C^{1,3}X_{Fuc} C^{2,4}X_{Fuc} ^{0,2}A_{Man}
1614.5 113.0
1615.6 117.0
1625.5 112.0

Annotations:

Y
1625.7 228.0

Annotations:

Y
1632.3 200.0
1632.7 170.0
1645.1 328.0
1652.6 610.0

Annotations:

B
1653.7 488.0

Annotations:

Y^{1,5}X_{Gal} ^{1,5}X_{Gal}
1656.8 113.0
1670.7 2108.0

Annotations:

C
1671.2 135.0
1671.6 1422.0
1672.6 1158.0
1673.7 269.0
1674.6 1085.0
1675.6 703.0

Annotations:

BZ
1675.8 554.0

Annotations:

BZ
1676.0 159.0

Annotations:

BZ
1676.5 496.0
1676.7 777.0
1677.4 143.0

Annotations:

^{0,2}X_{NeuAc}Z
1677.6 246.0

Annotations:

^{0,2}X_{NeuAc}Z
1692.5 210.0
1692.7 130.0
1693.6 330.0

Annotations:

CZ BY
1694.7 106.0
1695.7 127.0

Annotations:

^{1,5}X_{NeuAc}Z Z^{0,3}X_{Fuc} Z^{3,5}X_{Gal} ^{0,2}X_{NeuAc}Y
1707.6 111.0

Annotations:

^{0,3}X_{NeuAc}Z
1710.6 166.0
1711.2 135.0

Annotations:

CY
1711.8 336.0

Annotations:

CY
1712.6 684.0

Annotations:

^{2,4}A_{GlcNAc}
1714.4 173.0
1729.6 117.0

Annotations:

Y^{3,5}X_{Fuc}
1733.5 108.0
1733.9 215.0
1734.6 1581.0

Annotations:

ZZ
1735.7 1187.0

Annotations:

B^{0,2}X_{Gal} B^{0,4}X_{NeuAc}
1736.4 187.0
1736.7 447.0
1737.4 109.0

Annotations:

B^{1,5}X_{Fuc}
1737.6 326.0

Annotations:

B^{1,5}X_{Fuc}
1737.9 120.0

Annotations:

B^{1,5}X_{Fuc}
1748.8 133.0
1769.7 320.0

Annotations:

Z
1772.8 225.0

Annotations:

C^{2,4}X_{NeuAc} ^{0,2}A_{GlcNAc}
1787.8 706.0

Annotations:

Y
1788.5 393.0
1788.7 241.0
1812.3 108.0

Annotations:

Y^{0,2}X_{Gal} ^{0,2}X_{Fuc}Y ^{0,4}X_{NeuAc}Y
1813.7 114.0

Annotations:

C^{0,4}X_{Gal} C^{1,3}X_{Gal} C^{2,4}X_{Gal} C^{1,3}X_{Fuc} C^{2,4}X_{Fuc} ^{2,4}X_{NeuAc}Z
1838.6 148.0

Annotations:

^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z
1855.6 559.0

Annotations:

B
1856.7 718.0

Annotations:

^{1,3}X_{Fuc}Y ^{2,4}X_{Fuc}Y
1857.8 357.0

Annotations:

^{0,2}X_{NeuAc}
1858.7 197.0

Annotations:

Y^{3,5}X_{Gal} ^{3,5}X_{Fuc}Y
1873.8 123.0

Annotations:

C
1875.5 130.0
1898.6 160.0

Annotations:

Z
1914.8 122.0

Annotations:

Z

There are 10 similar spectra:

Id Structure Score

371

0.78

370

0.78

2363

0.68

2360

0.65

2246

0.63

2361

0.63

2362

0.58

2367

0.51

2539

0.51

2365

0.50
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