Sample handling of gastric tissue and O-glycan alterations in paired gastric cancer and non-tumorigenic tissues

Adamczyk B, Jin C, Polom K, Muñoz P, Rojas-Macias MA, Zeeberg D, Borén M, Roviello F, Karlsson NG, Scientific Reports, 2018, (8): 2045 - 2322

PubMed Entry:

Retention Time Recorded Precursor Mass Structure
8.01 513.25 [M -1H]1-
9.83 675.28 [M -1H]1-
10.8 716.32 [M -1H]1-
10.84 587.24 [M -1H]1-
11.51 1024.36 [M -1H]1-
12.53 628.25 [M -1H]1-
12.58 895.32 [M -1H]1-
12.63 425.32 [M -1H]1-
12.63 587.28 [M -1H]1-
12.89 675.3 [M -1H]1-
13.49 966.29 [M -1H]1-
13.86 716.37 [M -1H]1-
13.99 1024.4 [M -1H]1-
14.19 790.33 [M -1H]1-
14.25 1057.44 [M -1H]1-
14.79 1098.38 [M -1H]1-
14.86 749.34 [M -1H]1-
15.38 804.47 [M -2H]2-
15.86 733.36 [M -1H]1-
15.96 1406.48 [M -1H]1-
16.05 952.39 [M -1H]1-
16.05 878.32 [M -1H]1-
16.05 936.54 [M -1H]1-
16.38 775.97 [M -2H]2-
16.52 813.34 [M -1H]1-
16.89 958.47 [M -2H]2-
16.89 1186.61 [M -1H]1-
17.01 895.4 [M -1H]1-
17.09 905.81 [M -2H]2-
17.13 1155.45 [M -1H]1-
17.13 1260.47 [M -1H]1-
17.37 975.37 [M -1H]1-
17.74 804.46 [M -2H]2-
17.98 885.95 [M -2H]2-
18.19 986.96 [M -2H]2-
18.22 952.44 [M -1H]1-
18.34 1463.52 [M -1H]1-
18.96 1447.55 [M -1H]1-
18.96 1317.49 [M -1H]1-
18.96 1477.47 [M -1H]1-
19.09 994.06 [M -2H]2-
19.22 1666.66 [M -1H]1-
19.33 994.48 [M -2H]2-
19.46 1031.54 [M -2H]2-
19.46 895.45 [M -1H]1-
19.46 733.44 [M -1H]1-
19.47 1520.54 [M -1H]1-
19.58 1015.76 [M -2H]2-
19.58 1114.44 [M -1H]1-
20.82 1098.49 [M -1H]1-
20.82 739.47 [M -2H]2-
20.97 848.95 [M -2H]2-
21.08 1114.47 [M -1H]1-
21.34 1186.44 [M -1H]1-
21.59 1331.47 [M -1H]1-
21.59 804.59 [M -2H]2-
21.84 1260.5 [M -1H]1-
22.01 921.86 [M -2H]2-
22.22 885.53 [M -2H]2-
22.46 775.99 [M -2H]2-
22.46 775.99 [M -2H]2-
22.6 530.47 [M -1H]1-
22.69 1139.49 [M -1H]1-
22.85 1121.47 [M -1H]1-
22.85 1121.47 [M -1H]1-
22.85 812.51 [M -2H]2-
23.1 733.5 [M -1H]1-
23.22 821.46 [M -1H]1-
23.34 895.52 [M -1H]1-
23.34 1203.53 [M -1H]1-
23.47 1244.52 [M -1H]1-
23.83 936.53 [M -1H]1-
24.08 1031.55 [M -2H]2-
24.58 1406.55 [M -1H]1-
24.58 1406.55 [M -1H]1-
24.7 885.58 [M -2H]2-
24.7 958.55 [M -2H]2-
25.13 1041.55 [M -1H]1-
25.19 1260.54 [M -1H]1-
25.6 1187.43 [M -1H]1-
26.17 885.59 [M -2H]2-

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Homo Sapiens
Tissue - Gastric Mucosa

HPLC

Column Custom made Hypercarb

Method

  • 45 gradient with 10 ul/min run rate
  • 10 mM Ammonium Bicarbonate
  • 10 mM Ammonium Bicarbonate in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

UniCarb-DB data licensed under Creative Commons License CC BY 4.0

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