Detailed O-glycomics of the Muc2 mucin from colon of wild-type, core 1- and core 3-transferase-deficient mice highlights differences compared with human MUC2

Thomsson KA, Holmen-Larsson JM, Angstrom J, Johansson ME, Xia L, Hansson GC, Glycobiology, 2012, (22): 1128 - 1139

PubMed Entry:

Retention Time Recorded Precursor Mass Structure
15.7 384.0 [M -1H]1-
19.2 587.0 [M -1H]1-
19.4 767.0 [M -2H]2-
19.5 559.0 [M -2H]2-
20.2 685.0 [M -2H]2-
20.3 665.0 [M -2H]2-
21.0 787.0 [M -2H]2-
22.0 790.0 [M -1H]1-
22.2 975.0 [M -1H]1-
22.4 1243.0 [M -1H]1-
22.4 667.0 [M -1H]1-
22.8 749.0 [M -1H]1-
23.1 941.0 [M -2H]2-
23.1 1040.0 [M -1H]1-
23.4 733.0 [M -1H]1-
24.0 895.0 [M -1H]1-
24.5 975.0 [M -1H]1-
24.7 840.0 [M -2H]2-
24.8 829.0 [M -1H]1-
24.8 738.0 [M -2H]2-
25.9 1186.0 [M -1H]1-
26.0 1323.0 [M -1H]1-
26.1 829.0 [M -1H]1-
27.1 895.0 [M -1H]1-
29.2 1244.0 [M -1H]1-
31.1 1041.0 [M -1H]1-
31.9 530.0 [M -1H]1-
32.4 733.0 [M -1H]1-
32.8 1041.0 [M -1H]1-
35.3 975.0 [M -1H]1-
35.3 1389.0 [M -1H]1-
35.7 975.0 [M -1H]1-
35.7 1186.0 [M -1H]1-
36.1 813.0 [M -1H]1-
36.1 1121.0 [M -1H]1-
36.2 975.0 [M -1H]1-
37.6 884.0 [M -2H]2-
40.0 782.0 [M -2H]2-
42.1 1266.0 [M -1H]1-
43.4 734.0 [M -2H]2-

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Mus Musculus
Tissue - Colon
Glycoprotein - Muc2

HPLC

Column Custom made Hypercarb

Method

  • 60 gradient with 10 run rate
  • 10mM Ammonium Bicarbonate
  • 80% Acetonitrile in 10mM Ammonium Bicarbonate

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

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