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Recombinant mucin-type fusion proteins with Galα1,3Gal substitution as Clostridium difficile toxin A inhibitors

GlyToucan Accession Number: G76320TN

Peak	Intensity
108.94	0.7
112.07	3.4
113.1	6.6
114.1	10.9
119.14	2.0
124.48	0.9
125.15	1.3
126.06	33.2
126.56	0.8
126.97	1.2
131.02	3.8
136.91	1.2
138.11	24.3
139.11	1.5
142.95	11.4
143.16	7.3
143.96	1.8
149.15	0.6
156.08	132.2
157.0	6.7
157.26	2.3
161.08	13.6
Annotations: B
162.17	0.9
169.15	0.7
179.06	83.7
Annotations: C
180.07	2.0
186.13	5.9
198.98	0.4
202.14	0.9
204.09	404.2
Annotations: Z
204.74	6.4
205.12	19.0
222.33	13.6
Annotations: Y
223.23	2.6
227.21	0.8
227.97	0.7
234.99	0.7
236.88	0.8
237.25	3.2
244.11	2.3
244.93	0.9
245.3	0.6
249.1	2.1
250.99	1.3
261.04	3.9
263.35	1.7
264.08	2.0
271.09	1.2
275.72	3.0
276.19	27.6
280.04	0.9
283.78	1.5
293.95	0.7
297.95	0.9
299.12	2.3
304.34	0.8
310.94	1.3
313.12	3.3
314.17	0.7
315.2	1.2
317.79	1.2
319.13	0.7
321.88	0.5
324.24	15.1
330.26	0.9
340.21	1.3
342.03	0.5
347.25	1.0
352.53	2.5
356.4	0.7
358.96	2.1

There are 8 similar spectra:

Id	Structure	Score
325		1.00
2601		1.00
247		1.00
3029		1.00
230		0.99
171		0.99
91		0.97
2765		0.95

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass 383.16
(Calculated Monoisotopic)

Reducing End Reduced

Persubstitution Underivatised

Biological Context

Taxonomy - Cricetulus Griseus

Tissue - Cell Line, Transformed

HPLC

Column Custom made Hypercarb

Method

78 gradient with 10 run rate
10 mM Ammonium Bicarbonate
10 mM acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

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