MIRAGE upload

O-glycan repertoires on a mucin-type reporter protein expressed in CHO cell pools transiently transfected with O-glycan core enzyme cDNAs

GlyToucan Accession Number: G95567CI

Peak	Intensity
126.04	5.4
127.03	0.2
127.3	0.2
136.98	0.1
138.09	6.9
139.22	0.4
142.06	2.9
143.07	0.1
149.95	0.1
153.08	0.1
155.38	0.2
156.06	34.6
157.05	0.7
157.94	0.1
159.48	0.1
159.76	0.3
160.26	0.3
162.11	0.6
172.18	0.1
174.21	0.1
182.07	0.2
186.32	0.3
199.79	0.2
200.18	0.2
202.07	10.7
Annotations: B
203.09	0.9
204.07	161.1
Annotations: Z
205.03	5.5
205.51	0.2
212.93	0.1
218.95	0.1
219.99	6.5
Annotations: C
220.5	0.2
Annotations: C
221.11	0.1
222.09	15.7
Annotations: Y
222.97	0.9
223.32	0.2
224.0	0.1
238.96	0.1
244.05	0.1
257.49	0.2
275.06	0.3
278.1	0.3
280.8	0.1
281.19	0.1
282.9	0.1
291.69	0.1
294.84	0.1
297.86	0.1
301.2	0.2
304.85	0.4
305.24	0.2
310.17	0.1
312.16	0.2
317.12	13.6
318.08	1.1
321.0	0.2
321.76	0.1
323.97	0.4
324.35	0.1
325.11	0.2
326.16	0.1
327.21	0.1
329.03	0.1
333.78	0.2
334.93	0.3
335.19	0.2
335.8	0.3
336.11	0.1
338.32	0.1
338.72	0.1
339.13	0.1
339.96	0.3
341.35	0.1
341.84	0.2
345.22	0.2
349.97	0.1
352.34	0.2
352.99	0.1
353.8	0.1
354.04	0.2
355.61	0.1
360.57	0.1
361.11	0.1
362.03	0.2
362.8	0.1
365.16	6.0
366.24	0.3
380.18	0.1
380.81	0.5
381.2	0.1
381.86	0.5
383.28	0.2
385.26	0.2
389.05	0.2
389.23	0.1
390.35	0.1
394.97	0.2
395.82	0.2
397.01	0.1

There are 6 similar spectra:

Id	Structure	Score
2766		0.99
3030		0.99
447		0.99
92		0.96
3718		0.75
3031		0.51

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass 424.18
(Calculated Monoisotopic)

Reducing End Reduced

Persubstitution Underivatised

Biological Context

Taxonomy - Cricetulus Griseus

Tissue - Cell Line, Transformed

HPLC

Column Custom made Hypercarb

Method

Run time not recorded
50mM Ammonium Formate pH4.4
Acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

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