Mucin-type proteins produced in the Trichoplusia ni and Spodoptera frugiperda insect cell lines carry O-glycans with phosphocholine and sulfate substitutions
| Peak | Intensity |
|---|---|
| 191.11 | 0.1 |
| 192.94 | 0.1 |
|
Annotations: CY |
|
| 202.14 | 0.3 |
|
Annotations: B |
|
| 204.09 | 2.2 |
|
Annotations: Z |
|
| 205.05 | 0.1 |
| 205.23 | 0.1 |
| 207.94 | 0.1 |
| 208.17 | 0.1 |
| 210.06 | 0.1 |
| 212.09 | 0.1 |
| 217.2 | 0.1 |
| 217.99 | 0.1 |
| 219.95 | 0.3 |
|
Annotations: C |
|
| 220.16 | 0.2 |
|
Annotations: C |
|
| 222.12 | 2.5 |
|
Annotations: Y |
|
| 222.79 | 0.2 |
| 223.19 | 1.4 |
| 225.12 | 0.1 |
| 227.26 | 0.1 |
| 229.05 | 0.1 |
| 232.17 | 0.2 |
| 233.96 | 0.1 |
| 242.18 | 0.2 |
| 244.07 | 0.1 |
| 246.73 | 0.1 |
| 248.17 | 0.1 |
| 249.24 | 0.1 |
| 250.1 | 0.1 |
|
Annotations: ^{1,5}X_{HexA} |
|
| 254.49 | 0.1 |
| 255.03 | 2.1 |
| 256.03 | 0.5 |
| 256.81 | 0.1 |
| 258.15 | 0.1 |
| 258.98 | 0.2 |
| 260.73 | 0.1 |
| 262.01 | 0.1 |
|
Annotations: ^{2,4}A_{HexA} |
|
| 262.28 | 0.1 |
|
Annotations: ^{2,4}A_{HexA} |
|
| 264.07 | 0.2 |
|
Annotations: ^{0,2}X_{HexA} |
|
| 264.84 | 0.1 |
| 265.33 | 0.1 |
| 266.02 | 0.2 |
| 267.09 | 0.1 |
| 270.16 | 0.2 |
| 271.24 | 0.5 |
| 272.3 | 0.4 |
| 273.4 | 0.3 |
| 279.96 | 0.1 |
|
Annotations: ^{2,5}X_{HexA} |
|
| 280.27 | 0.2 |
|
Annotations: ^{2,5}X_{HexA} |
|
| 282.06 | 83.3 |
|
Annotations: B |
|
| 283.05 | 7.0 |
| 284.02 | 1.1 |
| 284.25 | 0.6 |
| 285.13 | 0.1 |
| 288.16 | 0.1 |
| 292.23 | 0.4 |
|
Annotations: ^{1,4}A_{HexA} |
|
| 293.13 | 0.1 |
| 295.22 | 0.1 |
| 296.1 | 0.1 |
| 296.83 | 0.1 |
| 297.16 | 0.2 |
| 297.79 | 0.3 |
| 298.17 | 0.8 |
| 298.5 | 0.1 |
| 298.87 | 0.6 |
| 299.25 | 0.4 |
| 300.01 | 3.2 |
|
Annotations: C |
|
| 300.4 | 0.4 |
|
Annotations: C |
|
| 301.11 | 1.6 |
| 302.1 | 138.8 |
| 303.09 | 15.4 |
| 304.78 | 0.1 |
| 306.2 | 0.1 |
|
Annotations: ^{0,3}A_{HexA} |
|
| 307.03 | 0.1 |
| 311.09 | 0.1 |
| 312.95 | 0.1 |
| 314.05 | 0.1 |
| 315.06 | 0.1 |
| 317.0 | 0.1 |
| 318.03 | 0.5 |
| 318.27 | 0.4 |
| 320.87 | 0.1 |
| 321.74 | 0.1 |
| 322.43 | 0.1 |
| 324.01 | 0.1 |
| 324.9 | 0.1 |
| 325.31 | 0.1 |
| 326.1 | 0.1 |
| 331.29 | 0.1 |
| 331.95 | 0.5 |
| 332.3 | 0.6 |
| 333.13 | 1.2 |
| 333.72 | 0.1 |
| 334.15 | 0.1 |
| 336.16 | 7.9 |
|
Annotations: ^{0,2}A_{HexA} |
|
| 337.24 | 0.6 |
| 337.49 | 0.1 |
| 338.03 | 0.2 |
|
Annotations: ^{2,4}X_{HexA} |
|
| 338.25 | 0.1 |
|
Annotations: ^{2,4}X_{HexA} |
|
| 339.23 | 0.1 |
| 342.05 | 0.2 |
|
Annotations: ^{2,4}A_{HexA} |
|
| 342.61 | 0.1 |
| 343.1 | 0.1 |
| 344.02 | 0.3 |
| 345.01 | 0.1 |
| 345.43 | 0.2 |
| 349.33 | 0.1 |
| 352.04 | 0.2 |
| 352.82 | 0.1 |
| 354.29 | 0.1 |
| 357.52 | 0.1 |
| 359.01 | 0.2 |
| 361.16 | 0.1 |
| 362.16 | 1.6 |
| 362.72 | 0.2 |
| 362.96 | 0.3 |
| 363.35 | 0.1 |
| 363.59 | 0.1 |
| 366.56 | 0.1 |
| 368.3 | 0.1 |
| 369.8 | 0.1 |
|
Annotations: ^{3,5}A_{HexA} |
|
| 370.19 | 0.3 |
|
Annotations: ^{3,5}A_{HexA} |
|
| 370.81 | 0.1 |
| 373.95 | 0.1 |
| 375.0 | 0.1 |
| 375.97 | 0.3 |
| 376.62 | 0.1 |
| 377.05 | 0.7 |
| 378.14 | 5.1 |
|
Annotations: B |
|
| 379.18 | 1.3 |
| 380.17 | 89.0 |
|
Annotations: Z |
|
| 381.14 | 16.4 |
| 382.19 | 0.2 |
| 384.29 | 0.1 |
| 385.91 | 0.1 |
|
Annotations: ^{0,3}A_{HexA} |
|
| 388.16 | 0.1 |
| 391.05 | 0.1 |
| 391.26 | 0.1 |
| 392.0 | 0.1 |
| 393.85 | 0.2 |
| 395.18 | 0.3 |
| 396.18 | 6.7 |
|
Annotations: C |
|
| 397.02 | 4.2 |
| 397.31 | 3.6 |
| 398.19 | 13.1 |
|
Annotations: Y |
|
| 399.14 | 2.3 |
| 399.4 | 1.0 |
| 400.07 | 0.2 |
|
Annotations: ^{2,5}A_{HexA} |
|
| 400.41 | 0.2 |
|
Annotations: ^{2,5}A_{HexA} |
|
| 404.98 | 0.1 |
| 411.04 | 0.2 |
| 412.37 | 0.1 |
| 412.9 | 0.1 |
| 414.19 | 2.5 |
| 415.19 | 0.2 |
| 415.73 | 0.1 |
|
Annotations: ^{0,2}A_{HexA} |
|
| 416.3 | 0.2 |
|
Annotations: ^{0,2}A_{HexA} |
|
| 417.29 | 0.2 |
| 418.13 | 0.1 |
| 420.1 | 0.2 |
| 420.35 | 0.1 |
| 421.42 | 0.1 |
| 422.08 | 0.1 |
| 424.06 | 0.1 |
| 426.1 | 0.1 |
| 427.3 | 0.1 |
| 428.14 | 0.1 |
| 429.22 | 0.1 |
| 430.41 | 0.1 |
|
Annotations: ^{1,5}A_{HexA} |
|
| 431.33 | 0.3 |
| 432.18 | 0.1 |
| 434.19 | 0.1 |
| 435.38 | 0.1 |
| 435.6 | 0.1 |
| 436.27 | 0.1 |
| 437.29 | 0.1 |
| 438.29 | 0.1 |
| 440.02 | 0.3 |
| 441.47 | 0.1 |
| 443.07 | 0.1 |
| 443.76 | 0.1 |
| 444.32 | 0.1 |
| 445.18 | 0.1 |
| 446.14 | 0.1 |
| 449.95 | 0.1 |
| 451.4 | 0.1 |
| 451.82 | 0.1 |
| 452.28 | 0.1 |
| 453.93 | 0.1 |
| 454.23 | 0.1 |
| 456.03 | 0.6 |
| 456.75 | 0.1 |
| 457.09 | 0.4 |
| 458.12 | 240.1 |
|
Annotations: B |
|
| 459.12 | 36.7 |
| 460.27 | 0.4 |
| 461.36 | 0.3 |
| 463.14 | 0.1 |
| 466.0 | 0.2 |
| 468.15 | 0.2 |
| 468.36 | 0.2 |
| 469.1 | 0.2 |
| 469.4 | 0.1 |
| 471.97 | 0.1 |
| 472.8 | 0.1 |
| 473.56 | 0.1 |
| 474.28 | 0.4 |
| 475.05 | 0.1 |
| 475.39 | 0.3 |
| 476.29 | 0.1 |
|
Annotations: C |
|
| 476.91 | 0.3 |
| 477.21 | 1.0 |
| 478.15 | 25.6 |
| 479.1 | 3.5 |
| 480.25 | 0.3 |
| 480.9 | 0.2 |
| 482.11 | 0.1 |
| 484.98 | 0.5 |
| 488.14 | 0.5 |
| 488.94 | 0.2 |
| 491.03 | 0.1 |
| 491.6 | 0.2 |
| 493.85 | 0.1 |
| 494.85 | 0.2 |
| 495.09 | 0.1 |
| 497.05 | 0.3 |
| 497.41 | 0.1 |
| 497.83 | 0.2 |
| 498.41 | 0.5 |
| 499.19 | 0.9 |
| 500.27 | 0.5 |
| 501.14 | 0.3 |
| 502.05 | 0.1 |
| 502.41 | 0.2 |
| 503.2 | 0.1 |
| 504.88 | 0.1 |
| 505.43 | 0.1 |
| 505.83 | 0.3 |
| 506.21 | 0.4 |
| 507.24 | 0.3 |
| 507.75 | 0.2 |
| 508.24 | 0.1 |
| 508.78 | 0.1 |
| 509.64 | 0.1 |
| 510.17 | 0.1 |
| 511.93 | 0.1 |
| 512.28 | 0.3 |
| 513.05 | 0.3 |
| 516.0 | 0.1 |
| 516.42 | 0.1 |
| 516.88 | 0.2 |
| 520.17 | 0.2 |
| 521.0 | 0.1 |
| 522.13 | 0.5 |
| 524.95 | 0.1 |
| 525.33 | 0.1 |
| 526.27 | 0.1 |
| 527.57 | 0.1 |
| 528.14 | 0.3 |
| 529.8 | 0.2 |
| 531.94 | 0.1 |
| 534.03 | 0.2 |
| 534.82 | 0.2 |
| 535.45 | 0.2 |
| 536.21 | 0.2 |
| 537.27 | 0.3 |
| 540.47 | 0.1 |
| 540.86 | 0.1 |
| 541.3 | 0.1 |
| 542.07 | 0.2 |
| 544.29 | 0.6 |
| 544.56 | 0.1 |
| 545.13 | 0.3 |
| 546.86 | 0.1 |
| 548.25 | 0.1 |
| 548.92 | 0.3 |
| 550.04 | 0.2 |
| 550.34 | 0.1 |
| 551.34 | 0.1 |
| 552.09 | 0.2 |
| 552.95 | 0.4 |
| 553.36 | 0.1 |
| 553.85 | 0.1 |
| 554.23 | 0.2 |
| 554.55 | 0.2 |
| 556.21 | 0.2 |
| 556.42 | 0.1 |
| 557.13 | 0.5 |
| 557.37 | 0.4 |
| 558.36 | 0.3 |
| 558.99 | 0.1 |
| 560.44 | 0.2 |
| 561.05 | 0.1 |
| 562.15 | 0.1 |
| 562.98 | 0.2 |
| 563.33 | 0.2 |
| 564.21 | 0.1 |
| 564.46 | 0.2 |
| 565.06 | 0.1 |
| 566.03 | 0.1 |
| 566.32 | 0.2 |
| 567.0 | 1.1 |
| 567.52 | 0.2 |
| 567.93 | 0.2 |
| 568.17 | 0.1 |
| 569.43 | 0.2 |
| 570.29 | 0.1 |
| 571.38 | 0.1 |
| 571.81 | 0.1 |
| 572.53 | 0.1 |
| 573.97 | 0.3 |
| 574.16 | 0.1 |
| 575.25 | 0.2 |
| 575.65 | 0.1 |
| 578.47 | 0.1 |
| 579.42 | 0.1 |
| 579.83 | 0.2 |
| 581.25 | 1.6 |
| 582.24 | 6.2 |
| 583.27 | 9.1 |
| 584.43 | 0.9 |
| 585.75 | 0.1 |
| 587.2 | 0.1 |
| 588.46 | 0.2 |
| 589.13 | 0.1 |
| 590.23 | 0.2 |
| 590.86 | 0.1 |
| 591.18 | 0.2 |
| 591.47 | 0.3 |
| 592.01 | 0.1 |
| 592.29 | 0.1 |
| 592.73 | 0.1 |
| 595.34 | 0.1 |
| 596.0 | 0.2 |
| 596.95 | 0.2 |
| 597.21 | 0.1 |
| 598.23 | 0.3 |
| 599.24 | 32.7 |
| 600.24 | 140.2 |
| 601.25 | 17412.9 |
|
Annotations: |
|
| 602.24 | 3540.8 |
| 604.19 | 0.2 |
| 605.36 | 0.1 |
| 605.74 | 0.1 |
| 606.23 | 0.1 |
| 607.14 | 0.2 |
| 607.81 | 0.1 |
| 608.26 | 0.2 |
| 609.15 | 0.1 |
| 610.38 | 0.2 |
| 611.14 | 0.1 |
| 612.5 | 0.2 |
| 614.36 | 0.1 |
| 615.0 | 0.1 |
| 618.32 | 0.3 |
| 618.92 | 0.1 |
| 619.45 | 0.1 |
| 620.06 | 0.5 |
| 621.2 | 6.0 |
| 622.24 | 1.5 |
| 623.18 | 0.1 |
| 623.59 | 0.2 |
| 626.17 | 0.1 |
| 627.11 | 0.1 |
| 628.94 | 0.1 |
| 630.97 | 0.1 |
| 632.13 | 0.1 |
| 633.21 | 0.1 |
| 634.44 | 0.2 |
| 635.08 | 0.2 |
| 635.6 | 0.1 |
| 635.94 | 0.3 |
| 636.3 | 0.7 |
| 637.24 | 0.5 |
| 639.37 | 0.4 |
| 640.16 | 0.2 |
| 640.85 | 0.3 |
| 641.24 | 0.1 |
| 644.03 | 0.1 |
| 645.22 | 0.1 |
| 645.89 | 0.3 |
| 646.18 | 0.3 |
| 646.58 | 0.1 |
| 647.12 | 0.6 |
| 647.58 | 0.2 |
| 648.24 | 0.1 |
| 649.69 | 0.1 |
| 650.98 | 0.4 |
| 652.53 | 0.1 |
| 653.98 | 0.1 |
| 659.94 | 0.1 |
| 661.28 | 0.1 |
| 661.52 | 0.1 |
| 716.53 | 0.1 |