Mucin-type proteins produced in the Trichoplusia ni and Spodoptera frugiperda insect cell lines carry O-glycans with phosphocholine and sulfate substitutions
| Peak | Intensity |
|---|---|
| 202.02 | 0.3 |
|
Annotations: B |
|
| 204.03 | 0.8 |
|
Annotations: Z |
|
| 211.8 | 0.1 |
| 215.98 | 0.1 |
| 219.77 | 0.2 |
|
Annotations: C |
|
| 220.01 | 0.2 |
|
Annotations: C |
|
| 222.25 | 0.4 |
|
Annotations: Y |
|
| 223.17 | 0.9 |
| 224.3 | 0.2 |
| 225.14 | 0.1 |
| 228.87 | 0.1 |
| 238.16 | 0.1 |
| 238.69 | 0.1 |
| 239.09 | 0.1 |
| 239.88 | 0.1 |
| 242.12 | 0.3 |
| 250.29 | 0.1 |
|
Annotations: ^{1,5}X_{HexA} |
|
| 252.22 | 0.2 |
| 255.09 | 0.6 |
| 256.0 | 0.1 |
| 257.85 | 0.1 |
| 262.84 | 0.1 |
| 265.11 | 0.2 |
| 268.05 | 0.3 |
| 271.11 | 0.3 |
| 272.09 | 0.1 |
| 273.18 | 0.2 |
| 273.9 | 0.2 |
| 275.4 | 0.1 |
| 278.82 | 0.2 |
| 280.03 | 0.2 |
|
Annotations: ^{2,5}X_{HexA} |
|
| 280.32 | 0.2 |
|
Annotations: ^{2,5}X_{HexA} |
|
| 282.06 | 10.1 |
|
Annotations: B |
|
| 282.97 | 0.8 |
| 283.16 | 0.5 |
| 284.04 | 0.4 |
| 284.81 | 0.1 |
| 285.97 | 0.2 |
| 287.41 | 0.1 |
| 291.82 | 0.2 |
|
Annotations: ^{1,4}A_{HexA} |
|
| 292.15 | 0.1 |
|
Annotations: ^{1,4}A_{HexA} |
|
| 294.3 | 0.1 |
|
Annotations: ^{0,3}X_{HexA} |
|
| 296.14 | 0.2 |
| 300.03 | 0.5 |
|
Annotations: C |
|
| 301.12 | 0.2 |
| 302.08 | 35.1 |
| 303.09 | 4.1 |
| 303.98 | 0.1 |
| 305.95 | 0.1 |
|
Annotations: ^{0,3}A_{HexA} |
|
| 310.02 | 0.1 |
|
Annotations: ^{3,5}X_{HexA} |
|
| 310.87 | 0.2 |
| 313.36 | 0.1 |
| 313.92 | 0.1 |
| 316.48 | 0.1 |
| 318.07 | 0.4 |
| 319.92 | 0.1 |
|
Annotations: ^{2,5}A_{HexA} |
|
| 321.87 | 0.1 |
| 322.98 | 0.1 |
| 325.69 | 0.1 |
| 327.84 | 0.2 |
| 332.04 | 0.5 |
| 332.31 | 0.1 |
| 333.07 | 0.1 |
| 336.18 | 0.8 |
|
Annotations: ^{0,2}A_{HexA} |
|
| 336.64 | 0.1 |
| 337.08 | 0.4 |
| 337.42 | 0.1 |
| 338.1 | 0.1 |
|
Annotations: ^{2,4}X_{HexA} |
|
| 342.84 | 0.2 |
| 344.08 | 0.2 |
| 353.17 | 0.1 |
| 358.17 | 0.3 |
| 359.73 | 0.1 |
| 361.01 | 0.1 |
| 362.03 | 0.4 |
| 363.17 | 0.2 |
| 363.92 | 0.1 |
| 366.34 | 0.2 |
| 368.02 | 0.2 |
| 372.2 | 0.3 |
|
Annotations: ^{1,4}A_{HexA} |
|
| 373.88 | 0.3 |
| 375.0 | 0.2 |
| 376.25 | 0.1 |
| 377.94 | 0.6 |
|
Annotations: B |
|
| 378.25 | 0.5 |
|
Annotations: B |
|
| 379.02 | 0.3 |
| 380.18 | 16.6 |
|
Annotations: Z |
|
| 381.18 | 3.0 |
| 383.15 | 0.2 |
| 384.96 | 0.1 |
| 385.21 | 0.1 |
| 385.68 | 0.1 |
|
Annotations: ^{0,3}A_{HexA} |
|
| 389.92 | 0.1 |
| 392.3 | 0.2 |
| 393.02 | 0.1 |
| 393.99 | 0.5 |
| 394.41 | 0.1 |
| 395.88 | 0.3 |
|
Annotations: C |
|
| 396.22 | 1.2 |
|
Annotations: C |
|
| 397.2 | 1.3 |
| 398.34 | 1.3 |
|
Annotations: Y |
|
| 399.12 | 1.4 |
| 399.34 | 0.9 |
| 399.88 | 0.1 |
|
Annotations: ^{2,5}A_{HexA} |
|
| 400.19 | 0.4 |
|
Annotations: ^{2,5}A_{HexA} |
|
| 401.7 | 0.1 |
| 402.98 | 0.1 |
| 405.83 | 0.2 |
| 408.15 | 0.4 |
| 409.17 | 0.2 |
| 409.96 | 0.1 |
| 412.9 | 0.1 |
| 413.56 | 0.3 |
| 414.31 | 0.1 |
| 415.0 | 0.2 |
| 416.21 | 0.4 |
|
Annotations: ^{0,2}A_{HexA} |
|
| 416.96 | 0.1 |
| 417.97 | 0.2 |
| 418.98 | 0.1 |
| 420.17 | 0.1 |
| 422.98 | 0.1 |
| 424.19 | 0.3 |
| 426.86 | 0.2 |
| 428.22 | 0.1 |
| 428.97 | 0.2 |
| 432.0 | 0.1 |
| 433.01 | 0.1 |
| 435.18 | 0.2 |
| 436.25 | 0.2 |
| 437.09 | 0.2 |
| 438.08 | 0.1 |
| 438.41 | 0.4 |
| 439.08 | 0.2 |
| 443.55 | 0.1 |
| 445.17 | 0.1 |
| 446.14 | 0.3 |
| 448.1 | 0.1 |
| 448.68 | 0.1 |
| 449.98 | 0.1 |
| 450.9 | 0.3 |
| 453.26 | 0.2 |
| 453.9 | 0.1 |
| 456.0 | 0.1 |
| 456.32 | 0.2 |
| 458.12 | 41.4 |
|
Annotations: B |
|
| 459.08 | 8.6 |
| 459.57 | 0.4 |
| 460.18 | 0.9 |
| 461.05 | 0.2 |
| 461.39 | 0.1 |
| 461.75 | 0.1 |
| 462.18 | 0.1 |
| 462.64 | 0.1 |
| 463.05 | 0.1 |
| 466.28 | 0.1 |
| 469.9 | 0.1 |
| 472.18 | 0.2 |
| 473.12 | 0.2 |
| 475.17 | 0.2 |
| 475.85 | 0.1 |
|
Annotations: C |
|
| 476.23 | 0.2 |
|
Annotations: C |
|
| 477.03 | 0.2 |
| 477.99 | 5.4 |
| 478.28 | 2.8 |
| 479.04 | 0.6 |
| 479.28 | 0.6 |
| 480.88 | 0.2 |
| 482.03 | 0.1 |
| 482.74 | 0.2 |
| 483.73 | 0.2 |
| 483.92 | 0.2 |
| 484.8 | 0.1 |
| 485.89 | 0.4 |
| 487.32 | 0.2 |
| 488.05 | 0.3 |
| 488.86 | 0.4 |
| 490.02 | 0.1 |
| 491.04 | 0.1 |
| 491.66 | 0.1 |
| 492.0 | 0.3 |
| 493.1 | 0.2 |
| 493.42 | 0.1 |
| 494.07 | 0.2 |
| 496.65 | 0.1 |
| 497.54 | 0.1 |
| 498.27 | 0.4 |
| 501.05 | 0.4 |
| 501.98 | 0.4 |
| 503.12 | 0.2 |
| 504.25 | 0.2 |
| 504.52 | 0.1 |
| 505.13 | 1.3 |
| 505.51 | 0.2 |
| 506.13 | 0.5 |
| 506.52 | 0.2 |
| 506.97 | 0.2 |
| 509.03 | 0.1 |
| 511.31 | 0.3 |
| 511.92 | 0.1 |
| 513.07 | 0.2 |
| 513.89 | 0.2 |
| 517.1 | 0.4 |
| 518.09 | 0.1 |
| 519.95 | 0.4 |
| 521.97 | 0.2 |
| 522.72 | 0.7 |
| 524.26 | 0.2 |
| 524.83 | 0.5 |
| 525.53 | 0.7 |
| 527.11 | 0.8 |
| 530.12 | 0.2 |
| 530.92 | 0.2 |
| 532.26 | 0.1 |
| 532.81 | 0.4 |
| 533.13 | 0.1 |
| 533.99 | 0.3 |
| 534.2 | 0.3 |
| 534.77 | 0.1 |
| 536.1 | 0.6 |
| 536.97 | 0.3 |
| 537.88 | 0.2 |
| 538.31 | 0.2 |
| 538.53 | 0.5 |
| 539.15 | 0.4 |
| 539.43 | 0.1 |
| 541.17 | 1.1 |
| 541.46 | 1.0 |
| 543.99 | 0.1 |
| 545.3 | 0.2 |
| 546.93 | 0.4 |
| 549.01 | 0.3 |
| 549.26 | 0.2 |
| 549.85 | 0.1 |
| 550.8 | 0.1 |
| 551.29 | 0.1 |
| 551.93 | 0.2 |
| 553.3 | 0.1 |
| 553.96 | 0.2 |
| 555.41 | 0.1 |
| 557.14 | 0.1 |
| 558.72 | 0.2 |
| 560.76 | 0.1 |
| 561.12 | 0.1 |
| 561.37 | 0.1 |
| 561.99 | 0.6 |
| 563.15 | 0.4 |
| 564.41 | 0.5 |
| 565.17 | 0.2 |
| 566.22 | 0.2 |
| 566.69 | 0.1 |
| 567.11 | 0.4 |
| 567.74 | 0.1 |
| 568.06 | 0.2 |
| 568.98 | 0.3 |
| 570.35 | 0.2 |
| 571.55 | 0.4 |
| 573.79 | 0.4 |
| 575.11 | 0.1 |
| 575.83 | 0.1 |
| 576.16 | 0.2 |
| 577.02 | 0.2 |
| 578.09 | 0.1 |
| 579.31 | 0.6 |
| 579.68 | 0.2 |
| 580.05 | 0.1 |
| 580.76 | 0.6 |
| 581.01 | 0.1 |
| 582.43 | 0.3 |
| 583.19 | 5.2 |
| 584.24 | 1.5 |
| 584.74 | 0.2 |
| 586.23 | 0.2 |
| 587.91 | 0.1 |
| 588.71 | 0.4 |
| 589.11 | 0.2 |
| 589.39 | 0.1 |
| 589.76 | 0.1 |
| 590.12 | 0.2 |
| 591.05 | 0.2 |
| 591.51 | 0.3 |
| 591.87 | 0.4 |
| 592.15 | 0.4 |
| 592.87 | 0.1 |
| 593.91 | 0.1 |
| 594.2 | 0.1 |
| 594.93 | 0.1 |
| 599.21 | 2.9 |
| 600.22 | 17.8 |
| 601.25 | 2264.9 |
|
Annotations: |
|
| 602.25 | 480.2 |
| 603.83 | 0.2 |
| 604.25 | 0.1 |
| 604.8 | 0.1 |
| 605.74 | 0.3 |
| 606.16 | 0.5 |
| 607.19 | 0.1 |
| 608.11 | 0.4 |
| 608.49 | 0.2 |
| 608.98 | 0.2 |
| 610.07 | 0.3 |
| 611.23 | 0.4 |
| 611.8 | 0.1 |
| 613.07 | 0.1 |
| 613.96 | 0.2 |
| 615.08 | 0.1 |
| 616.52 | 0.1 |
| 616.97 | 0.1 |
| 617.35 | 0.1 |
| 617.65 | 0.1 |
| 617.92 | 0.4 |
| 618.95 | 0.3 |
| 619.33 | 0.5 |
| 619.76 | 0.5 |
| 620.39 | 0.5 |
| 620.67 | 0.1 |
| 621.22 | 2.2 |
| 622.14 | 0.2 |
| 623.04 | 0.1 |
| 624.26 | 0.4 |
| 624.58 | 0.4 |
| 626.62 | 0.1 |
| 627.17 | 0.1 |
| 628.66 | 0.1 |
| 632.99 | 0.1 |
| 633.8 | 0.2 |
| 634.91 | 0.1 |
| 635.29 | 0.4 |
| 635.85 | 0.4 |
| 636.24 | 0.4 |
| 636.65 | 0.3 |
| 636.97 | 1.3 |
| 637.27 | 0.6 |
| 637.86 | 0.2 |
| 639.14 | 1.8 |
| 640.17 | 0.4 |
| 641.02 | 0.2 |
| 644.28 | 0.1 |
| 644.99 | 0.9 |
| 646.41 | 0.2 |
| 647.23 | 0.6 |
| 648.95 | 0.1 |
| 649.81 | 0.1 |
| 650.33 | 0.2 |
| 662.99 | 0.2 |
| 666.56 | 0.1 |
| 668.62 | 0.2 |