O-linked oligosaccharides from salivary agglutinin: Helicobacter pylori binding sialyl-Lewis x and Lewis b are terminating moieties on hyperfucosylated oligo- N-acetyllactosamine

PubMed Entry

GlyToucan Accession Number: G03266UM

Peak Intensity
225.8 15.0

Annotations:

B B
243.9 31.0
248.7 17.0

Annotations:

C^{0,2}X_{NeuAc} C^{0,2}X_{NeuAc}
261.9 26.0

Annotations:

^{2,5}X_{Gal}Z
262.7 17.0

Annotations:

^{3,5}A_{GlcNAc} Y^{2,4}X_{GlcNAc}
271.8 51.0
273.0 14.0
275.9 30.0

Annotations:

YZ ZY
287.9 29.0
288.6 68.0
289.9 1262.0

Annotations:

B
290.9 171.0

Annotations:

B^{1,5}X_{NeuAc}
291.9 53.0
295.6 10.0
302.8 27.0

Annotations:

Z^{2,5}X_{GlcNAc}
315.9 10.0
329.9 20.0

Annotations:

BZ
338.0 16.0
359.0 36.0

Annotations:

C^{2,4}X_{NeuAc} ^{0,2}A_{GlcNAc}
361.7 16.0
363.9 17.0

Annotations:

C^{1,4}X_{Fuc} ^{1,4}A_{GlcNAc}
366.1 11.0

Annotations:

CY YZ ZY YY YY
369.0 17.0

Annotations:

C^{2,4}X_{NeuAc} C^{2,4}X_{NeuAc}
370.6 12.0

Annotations:

B^{1,3}X_{Fuc} B^{2,4}X_{Fuc}
372.1 21.0

Annotations:

C^{0,3}X_{Fuc}
377.8 13.0

Annotations:

B^{1,3}X_{NeuAc} B^{0,4}X_{Fuc} Z^{0,2}X_{Gal} ^{0,2}X_{Gal}Z
379.9 18.0

Annotations:

C^{3,5}X_{Fuc} ^{1,4}A_{Gal} Y^{1,5}X_{Gal} ^{1,5}X_{Gal}Y
381.5 12.0
384.0 31.0

Annotations:

YY
384.9 12.0
389.0 10.0
404.0 10.0
405.9 10.0

Annotations:

B^{2,5}X_{Gal}
407.1 11.0

Annotations:

^{0,3}X_{NeuAc}Z Z^{0,3}X_{NeuAc}
423.9 84.0

Annotations:

^{1,5}A_{Gal} C^{2,5}X_{Gal} ^{3,5}X_{NeuAc}Y Y^{3,5}X_{NeuAc}
430.1 16.0

Annotations:

YZ ZY YZ ZY
444.1 16.0

Annotations:

^{1,5}X_{Fuc}Z Z^{1,5}X_{Gal} ^{1,5}X_{Gal}Z Z^{1,5}X_{Fuc}
445.9 12.0
459.8 10.0

Annotations:

^{0,2}X_{Fuc}Y Y^{0,2}X_{Gal} ^{0,2}X_{Gal}Y Y^{0,2}X_{Fuc}
470.1 63.0

Annotations:

C C
473.8 15.0

Annotations:

^{3,5}X_{Fuc}Z Z^{3,5}X_{Fuc} ^{1,5}X_{NeuAc}Z Z^{1,5}X_{NeuAc}
479.0 11.0
480.3 14.0

Annotations:

^{0,4}X_{Fuc}Z Z^{1,3}X_{Gal} Z^{2,4}X_{Gal} ^{1,3}X_{NeuAc}Z ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z Z^{1,3}X_{NeuAc} Z^{0,4}X_{Fuc}
492.1 38.0

Annotations:

BZ
507.2 11.0
507.9 10.0
509.1 98.0

Annotations:

Z^{2,4}X_{GlcNAc}
510.1 15.0

Annotations:

CZ BY
512.7 26.0
516.5 17.0
518.1 13.0
521.2 20.0
534.8 15.0

Annotations:

ZZ
538.9 11.0

Annotations:

^{1,4}X_{Fuc}Z Z^{1,4}X_{Fuc}
540.8 17.0

Annotations:

^{2,5}X_{Gal} ^{0,2}X_{Fuc}Y Y^{0,2}X_{Fuc} ^{2,5}X_{Gal}
542.1 42.0

Annotations:

^{0,3}A_{GlcNAc} ^{1,4}X_{NeuAc}Z Y^{2,4}X_{NeuAc} ^{2,4}X_{NeuAc}Y
551.1 40.0
552.1 15.0
553.0 31.0

Annotations:

YZ ZY
555.1 24.0

Annotations:

^{3,5}X_{Fuc}Z Y^{0,2}X_{NeuAc} ^{0,2}X_{NeuAc}Y Z^{3,5}X_{Fuc} ^{0,4}X_{NeuAc}Y
556.6 24.0

Annotations:

^{2,4}X_{NeuAc}Z
557.9 13.0
559.3 22.0
561.3 19.0

Annotations:

Z^{1,3}X_{NeuAc} Z^{0,4}X_{Fuc} ^{0,4}X_{Fuc}Z ^{1,3}X_{NeuAc}Z
565.4 19.0
569.2 21.0

Annotations:

Y^{0,3}X_{NeuAc} ^{0,3}X_{NeuAc}Y
571.1 29.0

Annotations:

YY
584.7 17.0
585.3 21.0
586.2 14.0
586.4 12.0
588.1 22.0
589.0 170.0
595.3 11.0

Annotations:

^{0,2}X_{Gal}Z Z^{0,2}X_{Gal}
597.7 12.0

Annotations:

C^{0,2}X_{NeuAc}
606.4 24.0
607.0 24.0
607.8 16.0

Annotations:

Y^{1,4}X_{NeuAc} ^{1,4}X_{NeuAc}Y
613.0 21.0

Annotations:

^{1,3}X_{NeuAc}Z ^{0,2}X_{Gal}Y Y^{0,2}X_{Gal}
616.4 12.0
618.5 18.0
621.9 27.0
622.6 13.0
625.9 24.0

Annotations:

B^{3,5}X_{NeuAc}
627.0 16.0
628.5 14.0

Annotations:

C^{0,3}X_{NeuAc} ^{1,5}X_{NeuAc} ^{1,5}X_{NeuAc}
631.7 17.0

Annotations:

^{1,3}X_{NeuAc}Y
634.5 25.0

Annotations:

Z^{1,3}X_{NeuAc} ^{1,3}X_{NeuAc}Z
636.6 10.0
637.0 20.0

Annotations:

BZ
639.7 49.0
640.0 24.0
641.1 29.0
645.7 22.0
652.1 16.0
654.1 14.0
655.1 32.0

Annotations:

BY CZ
656.0 34.0

Annotations:

Z
657.4 69.0

Annotations:

Z
660.4 14.0
662.5 28.0
664.1 19.0
665.2 75.0

Annotations:

Y
667.1 16.0
667.6 43.0
669.2 17.0
670.2 22.0
675.8 11.0
676.9 27.0
677.5 17.0
678.5 129.0

Annotations:

^{0,4}X_{NeuAc} ^{0,4}X_{NeuAc}
679.1 200.0

Annotations:

^{1,5}X_{Fuc}
680.0 40.0
682.1 22.0
684.4 23.0
685.5 16.0
687.5 17.0

Annotations:

^{2,4}X_{NeuAc} ^{2,4}X_{NeuAc}
688.9 20.0
692.4 13.0
693.9 75.0

Annotations:

^{2,5}X_{Fuc}
694.7 45.0

Annotations:

^{2,5}X_{Fuc}
696.3 11.0
697.0 25.0

Annotations:

ZZ ZZ
699.0 45.0

Annotations:

C^{0,4}X_{NeuAc}
699.5 19.0

Annotations:

C^{0,4}X_{NeuAc}
701.5 22.0

Annotations:

C^{1,5}X_{Fuc} ^{0,3}X_{Fuc}
704.9 33.0
705.7 37.0
706.5 72.0
707.1 117.0
707.4 97.0
708.1 105.0

Annotations:

^{1,3}X_{Fuc} ^{2,4}X_{Fuc}
708.8 75.0

Annotations:

^{3,5}X_{Fuc}
709.7 22.0

Annotations:

^{3,5}X_{Fuc}
710.7 40.0
711.4 25.0

Annotations:

B^{1,4}X_{Fuc}
711.7 24.0

Annotations:

B^{1,4}X_{Fuc}
713.0 14.0

Annotations:

B^{2,5}X_{Fuc}
715.2 106.0

Annotations:

YZ ZY YZ ZY
716.4 90.0

Annotations:

^{1,3}X_{NeuAc} ^{0,4}X_{Fuc} ^{1,3}X_{NeuAc}
717.7 46.0
718.5 30.0

Annotations:

C^{2,4}X_{NeuAc} ^{0,2}A_{GlcNAc}
719.6 16.0
724.1 17.0
728.2 98.0
729.2 163.0

Annotations:

C^{1,4}X_{Fuc} ^{1,4}A_{GlcNAc}
731.1 20.0

Annotations:

C^{2,5}X_{Fuc}
733.2 22.0

Annotations:

YY YY
737.6 15.0
740.6 17.0
754.2 11.0
756.1 18.0
764.6 12.0
780.5 12.0
793.0 12.0
801.2 27.0

Annotations:

B
803.4 20.0

Annotations:

^{0,2}X_{NeuAc}Y Y^{0,2}X_{NeuAc}
805.6 15.0

Annotations:

^{1,5}X_{NeuAc}Y Y^{1,5}X_{NeuAc} Y^{1,4}X_{Gal} ^{1,4}X_{Gal}Y
819.2 14.0

Annotations:

C
820.2 10.0
821.4 18.0
835.3 12.0

Annotations:

Y^{1,3}X_{Gal} Y^{2,4}X_{Gal} ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y
839.3 19.0
842.4 77.0

Annotations:

ZZ ZZ
843.1 13.0
844.2 14.0
854.0 25.0
857.3 13.0
864.1 15.0
869.3 21.0
873.0 17.0
875.4 12.0
877.3 13.0

Annotations:

YZ ZY
879.3 31.0
891.1 26.0

Annotations:

^{1,4}X_{NeuAc}Z Z^{1,4}X_{NeuAc}
893.0 26.0
894.4 30.0
895.2 736.0

Annotations:

YY
896.2 290.0
897.2 130.0
898.4 16.0
920.2 33.0

Annotations:

^{0,2}X_{Fuc}Y Y^{0,2}X_{Gal} ^{0,2}X_{Gal}Y Y^{0,2}X_{Fuc}
928.3 10.0
937.2 10.0
946.4 20.0

Annotations:

^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z Z^{1,3}X_{Fuc} Z^{2,4}X_{Fuc}
961.1 17.0
961.7 22.0
965.2 22.0

Annotations:

Y^{0,2}X_{NeuAc} ^{0,2}X_{NeuAc}Y
966.0 26.0

Annotations:

^{3,5}X_{Fuc}Y Y^{3,5}X_{Fuc}
970.0 13.0
981.7 23.0

Annotations:

^{2,5}X_{NeuAc}Y Y^{2,5}X_{NeuAc}
984.3 23.0
988.1 27.0
989.5 16.0
990.3 12.0
1004.2 42.0

Annotations:

ZZ ZZ
1005.2 28.0
1006.3 242.0

Annotations:

Z Z
1007.2 37.0
1008.4 22.0
1018.4 15.0
1022.5 34.0

Annotations:

YZ ZY YZ ZY
1023.1 12.0
1024.3 253.0

Annotations:

Y Y
1025.2 197.0
1026.3 34.0
1078.6 19.0

Annotations:

^{1,4}X_{Fuc}Z Z^{1,4}X_{Fuc}
1090.4 12.0
1108.3 437.0

Annotations:

Z^{2,5}X_{NeuAc} ^{2,5}X_{NeuAc}Z ^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z Z^{1,3}X_{Fuc} Z^{2,4}X_{Fuc}
1109.2 119.0
1110.3 34.0

Annotations:

^{3,5}X_{Fuc}Z Y^{0,2}X_{NeuAc} ^{0,2}X_{NeuAc}Y Z^{3,5}X_{Fuc}
1111.3 23.0
1113.2 65.0
1114.6 31.0
1115.1 18.0
1126.3 125.0

Annotations:

^{1,3}X_{Gal} ^{2,5}X_{NeuAc}Y Y^{2,5}X_{NeuAc} ^{2,4}X_{Gal} Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} ^{1,3}X_{Gal} ^{2,4}X_{Gal} ^{1,3}X_{Fuc}Y ^{2,4}X_{Fuc}Y
1127.3 28.0
1128.3 24.0

Annotations:

^{3,5}X_{Fuc}Y Y^{3,5}X_{Fuc}
1129.2 18.0
1129.9 11.0
1134.1 15.0
1135.0 20.0
1138.4 11.0

Annotations:

Z^{3,5}X_{NeuAc} ^{3,5}X_{NeuAc}Z
1142.3 43.0

Annotations:

Y^{1,3}X_{NeuAc} Y^{0,4}X_{Fuc} ^{0,4}X_{Fuc}Y ^{1,3}X_{NeuAc}Y
1144.3 15.0
1166.3 29.0
1167.2 32.0
1168.3 514.0

Annotations:

Z Z
1169.3 152.0
1170.3 50.0
1171.3 62.0
1172.2 14.0
1184.4 152.0
1185.2 26.0
1186.3 4662.0

Annotations:

Y Y
1187.3 2453.0
1188.3 810.0
1189.2 272.0
1190.2 70.0
1215.2 14.0
1226.3 15.0
1229.1 14.0
1276.2 16.0
1288.1 18.0
1292.4 16.0
1298.8 14.0
1300.3 33.0
1316.0 10.0

There are 5 similar spectra:

Id Structure Score

364

0.94

497

0.94

457

0.91

170

0.89

2470

0.87
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