O-linked oligosaccharides from salivary agglutinin: Helicobacter pylori binding sialyl-Lewis x and Lewis b are terminating moieties on hyperfucosylated oligo- N-acetyllactosamine

PubMed Entry

GlyToucan Accession Number: G18653BM

Peak Intensity
366.0 22.0

Annotations:

CY YZ ZY
371.0 35.0
372.0 15.0
373.1 23.0
383.2 33.0
384.1 420.0

Annotations:

YY
385.1 54.0
386.1 29.0
388.9 48.0
404.0 20.0

Annotations:

^{3,5}A_{GalNAc}Z
407.0 33.0
416.0 17.0
419.2 21.0
432.0 17.0
449.1 36.0

Annotations:

Z^{0,2}X_{GlcNAc}
449.8 24.0

Annotations:

B^{1,3}X_{Gal} B^{2,4}X_{Gal}
461.0 81.0
469.1 27.0
473.9 17.0
479.1 71.0
487.1 17.0
489.5 20.0
491.0 154.0
492.0 101.0

Annotations:

BZ
492.9 48.0
508.1 70.0
509.1 427.0

Annotations:

Z^{2,4}X_{GlcNAc}
509.9 89.0

Annotations:

CZ BY
511.2 35.0
521.1 227.0
522.4 48.0
523.1 29.0
532.0 17.0
533.1 72.0
534.1 61.0
535.0 19.0

Annotations:

ZZ
549.8 32.0
551.1 841.0
552.1 103.0

Annotations:

^{0,4}A_{GalNAc}Z
553.1 39.0

Annotations:

YZ ZY
554.1 23.0
569.1 31.0
571.2 71.0

Annotations:

YY
597.1 28.0
598.0 21.0

Annotations:

C^{0,2}X_{NeuAc}
631.1 33.0
636.0 29.0
646.0 20.0
654.5 49.0
655.1 45.0

Annotations:

BY CZ
656.1 548.0
657.2 254.0
658.1 28.0

Annotations:

^{2,4}A_{GlcNAc}
673.0 36.0

Annotations:

CY
674.2 20.0
675.1 26.0
687.2 16.0
691.2 17.0
697.1 57.0

Annotations:

ZZ ZZ ZZ
698.3 23.0
705.0 41.0
715.2 128.0

Annotations:

YZ ZY YZ ZY YZ ZY
716.1 359.0
717.1 134.0
718.1 26.0

Annotations:

^{0,2}A_{GlcNAc} C^{2,4}X_{NeuAc}
722.1 26.0
726.3 16.0
729.1 66.0

Annotations:

^{3,5}A_{GalNAc}Y C^{1,4}X_{Fuc} ^{1,4}A_{GlcNAc}
730.0 28.0
730.8 24.0

Annotations:

C^{2,5}X_{Fuc}
733.1 262.0

Annotations:

YY YY YY
734.1 175.0
735.3 32.0
736.1 32.0
758.1 25.0

Annotations:

^{0,2}A_{GalNAc}Z
758.7 16.0

Annotations:

^{0,2}A_{GalNAc}Z
759.2 21.0

Annotations:

C^{1,3}X_{Fuc} C^{2,4}X_{Fuc}
764.1 25.0
798.1 30.0
801.2 173.0

Annotations:

B
802.3 32.0
808.4 34.0
817.2 74.0

Annotations:

Z^{0,4}X_{Fuc} ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z ^{0,4}X_{Fuc}Z Z^{1,3}X_{Gal} Z^{2,4}X_{Gal} ^{1,3}X_{Gal}Z
818.3 47.0
819.3 732.0

Annotations:

C
820.2 180.0
821.1 29.0

Annotations:

^{3,5}X_{Fuc}Y Y^{3,5}X_{Fuc}
822.2 16.0
823.3 21.0
824.0 21.0
833.3 50.0

Annotations:

Y^{0,3}X_{NeuAc}
834.4 28.0
835.3 32.0

Annotations:

Y^{0,4}X_{Fuc} ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y ^{0,4}X_{Fuc}Y Y^{1,3}X_{Gal} Y^{2,4}X_{Gal} ^{1,3}X_{Gal}Y
837.3 105.0
838.4 22.0
839.3 15.0
841.1 17.0
842.2 34.0

Annotations:

ZZ
843.3 188.0
844.3 35.0
858.1 24.0
858.7 20.0
859.5 39.0

Annotations:

ZZ ZZ
860.2 52.0

Annotations:

YZ ZY
861.2 2629.0

Annotations:

Z
862.2 623.0
863.2 146.0
864.7 19.0
875.5 29.0

Annotations:

^{3,5}A_{GalNAc}
876.4 24.0
877.2 229.0

Annotations:

YZ ZY YZ ZY
879.3 4261.0

Annotations:

Y
880.3 1411.0
881.2 255.0
882.2 34.0
895.2 415.0

Annotations:

YY YY
896.2 199.0
897.3 37.0
903.2 51.0
920.8 34.0

Annotations:

^{0,2}A_{GalNAc}Z Y^{0,2}X_{Fuc}
921.4 23.0

Annotations:

^{0,2}X_{Gal}
922.3 20.0

Annotations:

^{2,5}A_{GalNAc}Y
933.3 23.0

Annotations:

^{1,4}X_{Fuc}Z Z^{1,4}X_{Fuc}
935.2 23.0

Annotations:

Z^{2,5}X_{Fuc} ^{2,5}X_{Fuc}Z
939.0 20.0
943.0 16.0
946.4 66.0

Annotations:

Z^{1,3}X_{Fuc} Z^{2,4}X_{Fuc}
947.3 66.0

Annotations:

^{0,2}X_{NeuAc}Z Z^{0,2}X_{NeuAc}
948.2 25.0

Annotations:

Z^{3,5}X_{Fuc}
961.2 14.0
963.2 15.0

Annotations:

^{2,5}X_{NeuAc}Z Z^{2,5}X_{NeuAc} Z^{1,3}X_{Fuc} Z^{2,4}X_{Fuc} ^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z
964.5 26.0

Annotations:

Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc}
965.3 69.0

Annotations:

Z^{3,5}X_{Fuc} ^{3,5}X_{Fuc}Z Y^{0,2}X_{NeuAc} ^{0,2}X_{NeuAc}Y
973.1 31.0
981.3 1645.0

Annotations:

^{2,5}X_{NeuAc}Y Y^{2,5}X_{NeuAc} ^{1,3}X_{Gal} ^{2,4}X_{Gal} Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} ^{1,3}X_{Fuc}Y ^{2,4}X_{Fuc}Y
982.3 614.0
983.2 78.0

Annotations:

Y^{3,5}X_{Fuc} ^{3,5}X_{Fuc}Y
985.4 22.0
987.2 28.0
989.3 107.0
990.3 33.0
994.3 14.0
999.3 452.0
1000.3 152.0
1001.3 31.0
1002.4 21.0
1003.2 68.0
1004.3 88.0

Annotations:

ZZ
1005.2 57.0
1006.2 72.0

Annotations:

Z
1007.2 36.0
1008.3 16.0
1019.3 75.0
1020.3 40.0
1022.3 39.0

Annotations:

YZ ZY
1023.3 337.0

Annotations:

Z
1024.3 1098.0

Annotations:

Y
1025.3 454.0
1026.2 142.0
1027.3 21.0
1035.1 14.0
1037.3 257.0
1038.3 149.0
1039.3 110.0
1041.3 24500.0

Annotations:

Y
1042.2 9155.0
1043.2 2059.0
1046.3 17.0
1059.4 16.0
1067.1 23.0

Annotations:

^{2,5}A_{GalNAc}Y ^{2,5}A_{GalNAc}Y ^{2,4}X_{NeuAc}Z Z^{2,4}X_{NeuAc}
1068.4 16.0

Annotations:

^{1,5}X_{Fuc}Y Y^{1,5}X_{Fuc}
1075.1 61.0
1076.3 14.0
1078.8 33.0

Annotations:

^{1,4}X_{Fuc}Z Z^{1,4}X_{Fuc}
1082.3 18.0

Annotations:

^{0,2}X_{Fuc}Y Y^{0,2}X_{Fuc}
1083.3 127.0

Annotations:

^{0,2}A_{GalNAc}Y ^{0,2}A_{GalNAc}Y
1084.3 96.0
1085.2 23.0

Annotations:

^{2,4}X_{NeuAc}Y Y^{2,4}X_{NeuAc}
1090.3 15.0
1091.2 22.0
1093.3 132.0
1094.3 57.0

Annotations:

^{0,3}X_{Fuc}Z Z^{0,3}X_{Fuc}
1095.3 27.0
1106.6 16.0
1108.3 81.0

Annotations:

^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z Z^{1,3}X_{Fuc} Z^{2,4}X_{Fuc}
1109.3 71.0
1111.3 107.0

Annotations:

^{0,2}X_{NeuAc}
1112.3 90.0

Annotations:

^{0,3}X_{Fuc}Y Y^{0,3}X_{Fuc}
1113.2 32.0

Annotations:

^{1,5}X_{NeuAc}
1126.4 33.0

Annotations:

^{1,3}X_{Fuc}Y ^{2,4}X_{Fuc}Y Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} ^{1,3}X_{Gal}
1127.4 21.0

Annotations:

^{2,5}X_{NeuAc}
1131.1 15.0
1134.3 793.0
1135.3 408.0
1136.3 149.0
1143.3 14.0
1147.3 18.0
1148.2 31.0
1152.3 15.0
1154.4 32.0
1156.1 29.0
1160.1 15.0
1164.3 150.0
1165.3 69.0
1166.4 35.0
1167.3 33.0
1168.3 151.0

Annotations:

Z Z
1169.3 21.0
1170.3 37.0
1171.2 53.0
1176.4 42.0
1177.2 64.0
1184.7 344.0
1185.5 224.0
1186.3 563.0

Annotations:

Y Y
1187.3 339.0
1188.3 108.0
1189.1 31.0
1192.3 39.0
1195.5 28.0
1200.8 31.0
1205.2 42.0
1206.3 34.0
1212.3 17.0

Annotations:

^{0,4}X_{NeuAc}
1213.1 20.0

Annotations:

^{2,5}A_{GalNAc}
1220.1 14.0
1221.7 17.0
1224.4 88.0
1225.7 29.0
1227.3 18.0
1228.3 25.0

Annotations:

^{0,2}X_{Fuc} ^{0,2}X_{Fuc}
1230.4 14.0
1234.1 24.0
1236.2 52.0
1237.3 40.0
1239.3 19.0
1251.6 25.0
1253.1 72.0
1254.3 7128.0
1255.3 2907.0
1256.3 1136.0
1261.7 20.0
1268.1 26.0
1271.1 74.0
1272.3 2628.0

Annotations:

^{1,3}X_{Fuc} ^{2,4}X_{Fuc} ^{1,3}X_{Fuc} ^{2,4}X_{Fuc}
1273.3 1171.0
1274.3 430.0

Annotations:

^{3,5}X_{Fuc} ^{3,5}X_{Fuc}
1278.2 24.0
1280.4 25.0
1281.7 25.0
1288.4 194.0

Annotations:

^{1,3}X_{NeuAc} ^{0,4}X_{Fuc} ^{0,4}X_{Fuc}
1289.2 43.0
1290.3 34.0
1291.4 20.0
1293.1 14.0
1294.3 24.0
1296.1 16.0
1297.1 36.0
1297.8 75.0
1298.8 45.0
1300.0 65.0
1301.3 38.0
1302.5 16.0
1303.1 19.0
1303.7 46.0
1306.9 20.0
1308.9 40.0
1309.6 54.0
1310.5 24.0
1311.4 43.0
1312.3 440.0
1313.3 191.0
1314.3 4508.0
1315.3 1793.0
1316.3 302.0
1317.4 56.0
1319.3 16.0
1321.2 44.0
1321.9 18.0
1326.3 15.0
1327.3 18.0

There are 6 similar spectra:

Id Structure Score

2831

0.98

2833

0.94

2900

0.88

2901

0.82

166

0.78

58

0.51
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