Mucin-type proteins produced in the Trichoplusia ni and Spodoptera frugiperda insect cell lines carry O-glycans with phosphocholine and sulfate substitutions

PubMed Entry

GlyToucan Accession Number: G93346UP

Peak Intensity
269.0 0.3
274.08 0.8

Annotations:

B^{2,5}X_{HexNAc}
297.35 0.8

Annotations:

^{3,5}A_{Gal}Z
313.43 0.3
322.17 0.4

Annotations:

C^{3,5}X_{HexNAc}
328.21 0.7

Annotations:

C^{2,5}X_{HexNAc}
337.09 17.5

Annotations:

BY CZ BY CZ BY CZ
337.62 0.2
338.21 0.9
354.4 1.5
355.18 0.9

Annotations:

CY CY CY
357.81 1.0

Annotations:

BY CZ BY CZ
364.18 0.4

Annotations:

BY BY CZ CZ
365.13 0.8

Annotations:

B^{1,5}X_{HexNAc}
366.26 0.8

Annotations:

Z
368.22 0.3
376.39 0.4
379.77 0.7

Annotations:

C
380.29 0.3

Annotations:

C
380.77 0.6

Annotations:

Y
384.24 1.2

Annotations:

Y
386.29 0.2
390.12 0.3
396.15 1.2

Annotations:

C
398.13 0.4
414.22 0.5

Annotations:

B^{0,3}X_{HexNAc}
418.39 0.2
427.59 0.6

Annotations:

C^{1,4}X_{HexNAc}
437.71 1.2

Annotations:

C^{2,4}X_{HexNAc} C^{0,2}X_{HexNAc} ^{2,4}A_{Gal}Y C^{0,4}X_{HexNAc}
438.64 3.3

Annotations:

BY CZ BY CZ
450.05 0.6

Annotations:

BZ
452.42 0.9

Annotations:

BY CZ
454.14 1.6

Annotations:

C^{2,5}X_{HexNAc}
460.13 1.5
462.34 1.9

Annotations:

Y
468.07 1.4

Annotations:

C
468.37 1.5

Annotations:

C
469.26 1.8
483.3 0.4

Annotations:

C^{1,4}X_{HexNAc}
493.27 0.2
498.23 4.9

Annotations:

C^{0,4}X_{HexNAc} C^{2,4}X_{HexNAc} ^{0,2}A_{Gal}Y C^{1,3}X_{HexNAc}
498.5 1.2

Annotations:

C^{0,4}X_{HexNAc} C^{2,4}X_{HexNAc} ^{0,2}A_{Gal}Y C^{1,3}X_{HexNAc}
498.97 0.7

Annotations:

C^{1,3}X_{HexNAc}
499.31 0.8
504.18 1.3

Annotations:

C^{0,3}X_{HexNAc} ^{0,3}A_{Gal}Y ^{1,4}A_{Gal}Y
504.9 0.7
513.88 1.1
514.15 1.5
517.23 0.8

Annotations:

^{1,5}A_{Gal}Z
520.12 0.2

Annotations:

^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y
521.54 1.0
521.9 0.5

Annotations:

BZ BZ BZ BZ BZ BZ
522.24 0.4

Annotations:

BZ BZ BZ BZ BZ BZ
524.06 3.0
524.36 1.9
531.11 2.1

Annotations:

BZ BZ BZ
533.2 0.5
535.24 1.2
536.87 0.4
538.21 0.3
540.24 6.8

Annotations:

B B
541.15 5.9

Annotations:

Z
541.73 2.2

Annotations:

Z
542.24 41.6

Annotations:

Z
543.22 8.4
544.42 0.7
546.21 0.3
547.74 0.4
548.99 0.5

Annotations:

C
549.6 3.2

Annotations:

C
549.82 1.1

Annotations:

Y
555.79 0.3
558.34 29.0

Annotations:

C
559.37 2.1
559.71 0.9

Annotations:

Y
560.17 9.7

Annotations:

Y
560.57 3.2

Annotations:

Y
561.22 1.6

Annotations:

^{2,4}A_{Gal}Z
566.25 0.4
566.55 0.3
572.04 0.5
573.31 0.8
578.4 0.5
579.47 0.4
580.22 0.5
580.73 0.5
581.32 1.2

Annotations:

B^{0,2}X_{HexNAc}
582.96 0.2

Annotations:

^{2,5}A_{Gal}Z
600.08 0.6

Annotations:

C^{1,3}X_{HexNAc} ^{2,4}A_{Gal}Y ^{0,2}A_{Gal}Y
607.0 0.4

Annotations:

^{1,5}A_{Gal}Y
614.25 0.9

Annotations:

^{3,5}A_{Gal}Y
615.96 0.3
618.45 0.5
619.18 0.2

Annotations:

BZ
619.75 0.3
620.17 0.5
623.26 0.6

Annotations:

B^{0,2}X_{HexNAc}
623.89 0.8
625.14 0.9
625.91 0.4

Annotations:

^{2,5}A_{Gal}Z
628.15 0.9

Annotations:

BY CZ
630.8 0.3
631.63 0.3
634.39 1.5
635.04 0.2
636.08 1.5
636.91 0.4

Annotations:

CY
638.25 0.3
639.05 0.8

Annotations:

B^{2,5}X_{HexNAc}
640.42 3.9
641.42 3.4

Annotations:

B
641.7 2.0

Annotations:

B
642.36 0.6

Annotations:

Z
642.74 0.5

Annotations:

Z
643.09 0.5

Annotations:

Z
646.32 0.2
647.97 0.3
650.21 1.7

Annotations:

C
650.84 6.1

Annotations:

C
651.19 0.9

Annotations:

C
651.59 0.2

Annotations:

Y
651.94 1.4

Annotations:

Y
652.2 0.5

Annotations:

Y
654.56 0.6
655.32 0.3
656.0 0.3

Annotations:

^{3,5}A_{Gal}Z ^{1,5}A_{Gal}Z
656.39 0.3

Annotations:

^{3,5}A_{Gal}Z ^{1,5}A_{Gal}Z
657.04 0.3

Annotations:

C^{2,5}X_{HexNAc}
658.31 0.2

Annotations:

^{2,4}A_{Gal}Y
660.16 0.7

Annotations:

^{0,2}A_{Gal}Y C^{1,3}X_{HexNAc}
660.99 1.5
661.26 0.6
667.33 0.9
669.26 0.3

Annotations:

B^{3,5}X_{HexNAc} B^{0,2}X_{HexNAc}
670.15 0.5

Annotations:

B^{0,2}X_{HexNAc}
672.08 0.6
672.41 1.1
672.91 0.3

Annotations:

^{0,3}A_{Gal}Y ^{1,4}A_{Gal}Y C^{1,4}X_{HexNAc}
674.51 1.4

Annotations:

^{1,5}A_{Gal}Y
678.35 0.3

Annotations:

C^{0,2}X_{HexNAc}
681.18 0.8
684.15 0.5

Annotations:

BZ BZ
687.78 0.5

Annotations:

C^{1,3}X_{HexNAc} ^{0,2}A_{Gal}Y
691.16 0.3
696.43 0.7
698.13 23.6

Annotations:

BZ BZ
699.12 2.5
699.54 2.5

Annotations:

B^{0,4}X_{HexNAc} B^{2,4}X_{HexNAc}
700.34 0.6

Annotations:

BZ
700.86 0.6

Annotations:

C^{0,4}X_{HexNAc} C^{2,4}X_{HexNAc}
701.24 0.3

Annotations:

C^{0,4}X_{HexNAc} C^{2,4}X_{HexNAc} C^{3,5}X_{HexNAc}
701.44 0.8

Annotations:

C^{0,4}X_{HexNAc} C^{2,4}X_{HexNAc} C^{3,5}X_{HexNAc}
702.37 0.4

Annotations:

BY CZ BY CZ
702.81 0.3

Annotations:

^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y ^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y
703.36 0.2

Annotations:

^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y
704.75 0.3
707.44 0.5
708.17 0.4
708.53 1.4

Annotations:

C^{0,4}X_{HexNAc} C^{2,4}X_{HexNAc}
708.76 1.2

Annotations:

BY CZ
709.25 1.5

Annotations:

BY CZ
713.26 0.4
713.98 0.8
716.08 2.0

Annotations:

BY CZ BY CZ
716.37 1.6

Annotations:

BY CZ BY CZ
716.98 0.7
717.24 1.2
717.48 0.3
718.07 0.4

Annotations:

CY
718.52 0.3

Annotations:

CY
719.46 1.7
720.54 1.7

Annotations:

CY CY
721.19 1.5
723.7 0.9

Annotations:

Z
724.12 0.3

Annotations:

Z
725.91 0.7
726.86 0.7
727.35 0.3
728.4 1.2
729.29 0.2

Annotations:

B
729.75 3.3

Annotations:

B
730.43 1.9
730.98 5.3
731.4 0.5
731.76 1.9

Annotations:

C^{1,5}X_{HexNAc}
732.25 1.1

Annotations:

Y
732.74 1.0

Annotations:

Y
733.24 1.9
733.61 1.2
734.3 1.0

Annotations:

CY CY
735.16 0.6
736.23 0.3
736.58 0.3
737.82 0.5
738.82 3.6

Annotations:

C
739.24 2.6
739.55 1.3
740.23 1.0
741.25 1.4
741.63 0.2
743.21 4.5

Annotations:

B
743.63 0.8

Annotations:

B
744.16 2.5

Annotations:

B^{1,5}X_{HexNAc}
746.24 0.7
747.33 1.7
748.04 0.7
748.81 0.5
749.25 0.3
750.33 0.6

Annotations:

B^{0,2}X_{HexNAc}
752.41 1.1
752.76 0.5
755.26 0.8
757.25 0.7
758.6 1.1

Annotations:

^{2,4}A_{Gal}Z B^{2,5}X_{HexNAc}
759.23 0.3

Annotations:

C^{0,2}X_{HexNAc} ^{2,4}A_{Gal}
759.86 1.1

Annotations:

C^{0,2}X_{HexNAc} ^{2,4}A_{Gal} B^{1,3}X_{HexNAc}
761.32 77.9

Annotations:

C
762.45 11.5

Annotations:

C^{1,5}X_{HexNAc}
763.35 8.2

Annotations:

Y
764.07 1.5
765.19 0.5
769.24 1.0

Annotations:

C^{1,3}X_{HexNAc}
772.99 0.4
776.98 0.3
778.15 0.8
780.44 0.6

Annotations:

B^{2,4}X_{HexNAc} B^{0,4}X_{Gal} B^{0,4}X_{HexNAc} B^{1,3}X_{Gal}
780.94 2.9

Annotations:

B^{0,4}X_{HexNAc} B^{2,4}X_{HexNAc} B^{0,4}X_{Gal} B^{1,3}X_{Gal}
781.86 0.3

Annotations:

^{2,5}A_{Gal} ^{0,4}X_{Gal}Z ^{1,3}X_{Gal}Z
784.21 0.5
785.17 0.8
785.57 0.8
788.24 1.1

Annotations:

B^{1,4}X_{HexNAc} ^{0,3}A_{Gal}Z ^{1,4}A_{Gal}Z
789.78 12.5

Annotations:

C^{0,4}X_{HexNAc} C^{2,4}X_{HexNAc} C^{0,4}X_{Gal} C^{1,3}X_{Gal} ^{0,2}A_{Gal} ^{3,5}A_{Gal}Y
790.61 1.9

Annotations:

^{3,5}A_{Gal}Y ^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y
791.01 1.8

Annotations:

^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y
791.51 0.5
793.33 1.1
793.97 0.7
795.05 1.0
798.99 0.3

Annotations:

B^{0,2}X_{HexNAc}
801.66 1.3
802.12 0.9

Annotations:

^{2,5}A_{Gal}Z
802.55 0.3

Annotations:

^{2,5}A_{Gal}Z
803.07 2.6
803.39 1.3
803.91 0.4
804.56 0.7
805.93 0.9

Annotations:

C^{1,4}X_{HexNAc} ^{0,3}A_{Gal}Y ^{1,4}A_{Gal}Y
806.17 0.4

Annotations:

C^{1,4}X_{HexNAc} ^{0,3}A_{Gal}Y ^{1,4}A_{Gal}Y
807.61 0.3
809.38 0.3
810.53 0.4

Annotations:

B
810.94 0.2

Annotations:

B
811.8 63.5

Annotations:

Z
812.26 21.6
812.91 4.3
813.39 4.1
813.85 0.3
815.15 1.1

Annotations:

B^{2,5}X_{HexNAc}
819.9 7.7

Annotations:

C
820.37 0.5

Annotations:

Y
821.01 9.3

Annotations:

Y
821.37 1.5
821.81 0.8
822.23 0.4
822.82 1.0
824.09 0.4
825.3 0.4
826.25 0.5
826.87 1.1
831.02 0.5
832.05 1.6

Annotations:

^{1,5}A_{Gal}Z
833.56 0.3

Annotations:

C^{2,5}X_{HexNAc}
834.22 4.3
834.95 0.7

Annotations:

^{1,5}X_{HexNAc}
835.31 2.2
836.18 1.7

Annotations:

C^{1,3}X_{HexNAc} ^{0,2}A_{Gal}Y
836.4 0.5

Annotations:

C^{1,3}X_{HexNAc} ^{0,2}A_{Gal}Y
836.93 0.7
837.28 0.5
839.58 0.6
840.32 2.7
840.66 1.8
841.44 5.3
842.34 18.1
843.29 7.6
843.75 0.6
845.81 1.3
846.56 0.2
847.44 0.6

Annotations:

C^{0,3}X_{HexNAc} ^{0,4}X_{Gal}Z ^{1,3}X_{Gal}Z
847.84 0.6
848.42 0.4
849.03 0.8
850.3 0.7

Annotations:

^{1,5}A_{Gal}Y
850.52 0.4

Annotations:

^{1,5}A_{Gal}Y
852.06 0.4
852.62 0.5
853.35 0.4
853.87 0.4
855.32 1.6
856.18 0.3
856.63 0.8

Annotations:

^{1,4}X_{HexNAc}
857.01 0.2

Annotations:

^{1,4}X_{HexNAc}
858.35 1.0
858.88 1.3

Annotations:

B^{0,4}X_{HexNAc} B^{2,4}X_{HexNAc}
859.37 1.1

Annotations:

B^{0,4}X_{HexNAc} B^{2,4}X_{HexNAc}
859.65 1.2

Annotations:

B^{0,4}X_{HexNAc} B^{2,4}X_{HexNAc}
860.12 3.1

Annotations:

BZ BZ
860.45 1.9

Annotations:

BZ BZ
860.91 0.9
861.28 1.0
861.93 2.9

Annotations:

^{0,2}X_{HexNAc}
862.39 0.3

Annotations:

^{0,2}X_{HexNAc}
862.73 0.3

Annotations:

^{0,2}X_{HexNAc}
864.31 1.5
867.15 0.4
868.55 0.3
869.27 0.7
869.86 0.6

Annotations:

^{2,5}X_{HexNAc}
873.02 1.5
873.27 1.1
874.94 1.2
875.76 0.6
876.16 0.2
876.56 0.8
877.39 7.2

Annotations:

C^{0,4}X_{HexNAc} C^{2,4}X_{HexNAc} ^{0,3}X_{HexNAc}
878.26 13.5

Annotations:

BY CZ BY CZ
878.6 4.8

Annotations:

BY CZ BY CZ
879.32 7.7
879.85 1.3
880.43 2.1
881.27 0.3
883.07 1.0
883.61 0.8
884.06 0.8
885.16 1.7

Annotations:

^{3,5}X_{HexNAc}
885.8 1.0
886.86 0.4

Annotations:

BZ
887.21 1.5

Annotations:

BZ
887.41 0.8

Annotations:

BZ
888.09 0.8
890.74 0.7
893.85 0.5
894.17 1.6
894.44 0.2
895.68 0.4
903.39 0.4
903.81 0.5
925.15 0.4

Annotations:

Y
937.43 1.4

Annotations:

C
938.1 0.9
957.47 0.5
959.08 0.6
977.65 0.4

Annotations:

B^{2,5}X_{HexNAc}
995.21 0.3

Annotations:

C^{2,5}X_{HexNAc}
996.41 0.2
1017.28 0.8
1019.39 0.8
1022.18 0.3
1023.62 0.5

Annotations:

^{2,5}A_{Gal}Y ^{0,4}X_{Gal}Z ^{1,3}X_{Gal}Z
1026.07 0.3
1036.66 0.5
1037.15 0.3
1037.59 0.3
1038.32 0.3
1039.34 79.9

Annotations:

C^{0,4}X_{HexNAc} C^{2,4}X_{HexNAc} ^{0,2}A_{Gal}Y
1040.43 18.3
1041.36 3.0

Annotations:

^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y
1042.31 0.5
1057.27 0.3
1057.85 1.1
1065.29 0.8
1067.21 0.8
1068.55 0.4
1069.39 0.3
1077.95 0.8
1079.08 0.7
1080.1 1.0
1080.39 1.0
1080.85 0.8

Annotations:

B
1081.39 6.9

Annotations:

B
1082.32 2.1
1083.36 77.4

Annotations:

Z
1084.31 23.5
1084.86 1.8
1085.21 5.9
1085.58 3.2
1086.21 1.0
1086.7 0.9
1095.53 0.2
1097.35 0.3
1098.16 1.1
1099.35 23.0

Annotations:

C
1099.7 8.7

Annotations:

C
1100.21 3.4
1100.58 2.6
1101.34 4.0

Annotations:

Y
1101.61 2.0

Annotations:

Y
1102.16 1.1
1102.47 1.1
1151.2 1.2
1169.45 1.1
1185.33 2.0

Annotations:

^{2,5}A_{Gal}Y
1194.95 0.8
1195.34 0.5
1228.45 0.7

Annotations:

C^{3,5}X_{HexNAc}
1238.93 0.4

Annotations:

BZ
1239.61 0.3

Annotations:

BZ
1241.48 0.7
1258.04 1.1
1261.63 0.2

Annotations:

CY
1284.39 3.7

Annotations:

B
1284.71 1.4

Annotations:

B
1285.03 0.3

Annotations:

B^{1,5}X_{HexNAc}
1286.39 2.8

Annotations:

Z
1289.53 0.2
1290.56 0.7
1300.35 0.4
1302.06 2.6

Annotations:

C
1302.46 16.5

Annotations:

C
1303.65 1.4

Annotations:

C^{1,5}X_{HexNAc}
1304.39 2.2

Annotations:

Y
1320.43 1.3
1326.42 0.3
1345.86 0.3
1384.27 1.2
1385.27 0.3
1419.45 0.6

Annotations:

BY CZ
1429.26 1.1
1430.75 0.9
1441.52 1.0
1448.33 1.5

Annotations:

Z
1448.6 0.6

Annotations:

Z
1461.58 0.3
1464.04 0.8
1464.39 0.5
1466.33 1.0

Annotations:

Y
1466.66 1.5

Annotations:

Y
1467.37 1.2
1468.36 1.8
1538.42 0.7
1578.39 0.9
1580.31 1.0

Annotations:

C^{0,4}X_{HexNAc} C^{2,4}X_{HexNAc} C^{0,4}X_{Gal} C^{1,3}X_{Gal} ^{0,2}A_{Gal}
1581.32 0.2
1581.62 0.3
1582.16 0.6

Annotations:

^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y
1582.54 0.9

Annotations:

^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y
1606.74 0.9
1619.91 0.3
1624.48 2.0

Annotations:

Z
1625.77 1.8
1727.6 1.2
1739.79 0.5

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