Efficient Chemoenzymatic Synthesis of an N-glycan Isomer Library

PubMed Entry

GlyToucan Accession Number: G62446JD

Peak Intensity
256.1 5.0
257.03 2.0
274.15 220.1
275.07 12.8
291.36 0.7
292.15 190.4

Annotations:

B
293.07 7.7

Annotations:

^{3,5}A_{Man}Z ^{1,4}A_{GlcNAc}
294.02 3.4

Annotations:

YY
294.74 1.1

Annotations:

^{0,4}A_{Man}Y
295.32 0.9
296.22 1.1
310.17 2.0

Annotations:

C
312.0 0.7
325.1 1.6

Annotations:

^{0,2}A_{Man}Y C^{1,3}X_{Man}
330.7 3.8
348.16 2.9

Annotations:

BZ BZ
364.68 1.7
365.29 12.3
366.19 958.7

Annotations:

B
367.2 90.6
368.14 0.4

Annotations:

C^{1,5}X_{GlcNAc} C^{2,4}X_{NeuAc}
370.54 2.8
371.11 0.7

Annotations:

C^{2,4}X_{NeuAc}
374.41 1.0

Annotations:

^{1,4}A_{Man}
383.2 1.3
384.16 4.0

Annotations:

C
395.62 0.7

Annotations:

B^{2,5}X_{GlcNAc}
400.2 1.6
407.32 5.3

Annotations:

Z
408.19 0.4

Annotations:

B^{0,2}X_{Gal} ^{1,3}A_{Man}Z ^{0,4}A_{Man}Z
418.19 1.2

Annotations:

^{2,5}A_{Man}Y
425.2 13.7

Annotations:

Y
425.92 1.8

Annotations:

^{1,3}X_{Man}Y ^{1,5}A_{Gal} C^{1,3}X_{Man} ^{1,3}A_{Man}Y ^{0,2}A_{Man}Y ^{0,4}A_{Man}Y ^{1,3}A_{Man} ^{2,4}A_{Man} C^{0,2}X_{Gal} C^{2,4}X_{GlcNAc} Y^{1,3}X_{Man} Y^{1,3}X_{GlcNAc}
436.17 2.8

Annotations:

B^{0,2}X_{NeuAc}
437.41 0.6

Annotations:

^{2,4}X_{GlcNAc}Z Z^{0,4}X_{GlcNAc} Z^{2,4}X_{GlcNAc}
447.27 2.9

Annotations:

BY CZ BY CZ ZY YZ
451.68 2.7

Annotations:

B^{2,5}X_{NeuAc}
452.49 1.2

Annotations:

B^{2,5}X_{NeuAc}
454.18 51.0

Annotations:

B
455.03 7.3

Annotations:

^{0,3}A_{Man}
460.33 1.1
467.79 1.8

Annotations:

Z
472.18 1.6

Annotations:

C
475.02 1.6
477.34 0.5

Annotations:

C^{0,2}X_{Gal}
487.29 1.5
494.24 1.4
495.31 38.2
496.16 6.8
505.27 2.2

Annotations:

B^{1,5}X_{Man} ^{1,5}A_{Man}Z C^{2,5}X_{GlcNAc} ^{2,5}X_{Gal}Y Y^{2,5}X_{GlcNAc} ^{2,5}X_{GlcNAc}Y
512.19 5.0
527.15 1.7

Annotations:

B^{1,4}X_{Man}
528.26 227.1

Annotations:

BY CZ BY BY CZ CZ
529.29 31.8
533.08 0.6
545.29 1.4
546.36 6.7

Annotations:

CY CY
547.25 2.4
548.85 4.7

Annotations:

Z
549.53 0.7

Annotations:

^{1,5}A_{Man}Z
551.67 1.1

Annotations:

ZZ
558.01 19.0

Annotations:

Y
560.32 1.9

Annotations:

C^{1,4}X_{NeuAc}
563.54 3.0

Annotations:

BZ
569.3 31.1

Annotations:

ZY YZ
570.22 2.2

Annotations:

^{0,4}A_{Man}Z
571.3 2.2

Annotations:

B^{2,5}X_{NeuAc} Y^{1,5}X_{Man} ^{1,5}X_{GlcNAc} Y^{1,5}X_{GlcNAc} C^{1,5}X_{Gal} ^{1,5}X_{Gal}Y
578.7 0.7

Annotations:

B^{0,3}X_{NeuAc} C^{0,2}X_{Gal} ^{0,2}X_{Gal}Y
584.57 0.9

Annotations:

^{3,5}A_{Man}Z ^{1,5}X_{NeuAc}Z Z^{1,4}X_{Man} Z^{1,4}X_{GlcNAc} ^{1,4}X_{Gal}Z B^{1,4}X_{Gal}
585.4 2.8

Annotations:

^{1,4}A_{GlcNAc}
586.68 1.3

Annotations:

B^{3,5}X_{NeuAc} B^{1,5}X_{GlcNAc} C^{2,5}X_{Gal} ^{2,5}X_{Gal}Y
587.33 52.3

Annotations:

YY
588.34 4.0

Annotations:

^{0,4}A_{Man}Y
589.51 1.3
591.61 1.5

Annotations:

^{2,5}X_{NeuAc}Z
592.83 1.9

Annotations:

BY CZ
602.59 6.8

Annotations:

^{3,5}A_{Man}Y
603.73 8.1
604.79 1.6

Annotations:

Z^{0,3}X_{GlcNAc}
610.33 0.9
615.53 0.4

Annotations:

Y^{1,5}X_{Man} ^{1,5}X_{Man}Y ^{3,5}X_{NeuAc}Y C^{1,5}X_{Man}
617.82 2.5

Annotations:

C^{1,4}X_{Man} C^{1,5}X_{NeuAc} ^{0,3}A_{Man}Y
622.22 2.6

Annotations:

^{3,5}X_{GlcNAc} Y^{3,5}X_{GlcNAc}
629.86 8.1

Annotations:

ZY YZ BY CZ
633.28 1.8
635.3 3.1
639.18 62.7

Annotations:

CY YY
640.27 3.9
641.44 1.6

Annotations:

Z^{1,4}X_{Man} ^{1,4}X_{Man}Z
643.61 2.3

Annotations:

^{0,4}X_{NeuAc}Y B^{1,3}X_{Man} B^{0,4}X_{GlcNAc} B^{2,4}X_{GlcNAc} ^{2,4}X_{NeuAc}Z Z^{1,5}X_{GlcNAc}
644.45 1.7

Annotations:

B^{3,5}X_{NeuAc} ^{1,5}A_{Man}Z ^{2,5}A_{Man}
650.34 2.0

Annotations:

Z
650.91 1.6
655.63 5.8
656.46 9.7
657.3 2336.3

Annotations:

B
658.31 388.8
659.27 27.0

Annotations:

Y
659.95 7.4

Annotations:

^{1,5}A_{Man}
660.83 1.7

Annotations:

C^{1,3}X_{NeuAc}
672.38 1.1

Annotations:

BZ
673.98 4.2

Annotations:

B
674.75 2.8

Annotations:

C
675.4 4.2

Annotations:

C
683.03 2.8

Annotations:

C
685.53 1.6
688.57 1.8

Annotations:

^{2,5}X_{Gal} Y^{2,5}X_{GlcNAc} C^{0,3}X_{NeuAc} ^{0,3}X_{NeuAc}Y
689.42 0.8

Annotations:

Y^{1,3}X_{Man} ^{1,3}X_{Man}Y Y^{1,3}X_{GlcNAc}
690.33 223.6

Annotations:

BY CZ
691.42 46.4
692.45 1.2
694.29 10.8

Annotations:

Z
694.8 2.6

Annotations:

^{1,4}X_{Gal} Y^{0,3}X_{GlcNAc}
695.41 4.0

Annotations:

^{1,4}X_{Gal} Y^{0,3}X_{GlcNAc}
698.05 3.8
699.34 1.3

Annotations:

B^{0,4}X_{NeuAc}
703.49 20.0

Annotations:

Y
704.48 4.8

Annotations:

B^{1,4}X_{NeuAc}
705.17 1.0
707.15 1.7
708.46 6.8

Annotations:

CY
710.07 0.6

Annotations:

B^{1,4}X_{GlcNAc} ^{1,3}X_{Gal} ^{2,4}X_{Gal} Y^{0,4}X_{GlcNAc} Y^{2,4}X_{GlcNAc}
713.48 2.1

Annotations:

BZ ZZ ZZ
723.14 0.8

Annotations:

B^{2,5}X_{GlcNAc}
730.37 9.6

Annotations:

B^{0,3}X_{GlcNAc}
731.4 271.8

Annotations:

Z
732.44 47.0

Annotations:

C^{2,5}X_{GlcNAc}
739.55 11.0

Annotations:

C^{0,3}X_{GlcNAc} ^{1,4}X_{Man}
740.47 403.2

Annotations:

Y
741.23 21.8
742.4 1.3
743.01 0.9
743.82 3.1
746.12 1.7
747.34 1.0

Annotations:

C^{3,5}X_{GlcNAc} ^{1,4}A_{Man}
748.46 10.7
749.37 379.4

Annotations:

CY YY YY
750.38 79.7
751.17 1.4
752.92 1.7

Annotations:

B^{1,3}X_{NeuAc} ^{1,3}X_{NeuAc}Z
756.71 0.9
760.61 1.8
766.24 11.7
766.89 3.5
775.44 61.6

Annotations:

B Z
775.96 5.9

Annotations:

^{1,5}X_{NeuAc}
776.87 2.1

Annotations:

C^{1,5}X_{Man} ^{1,5}X_{GlcNAc}Y Y^{1,5}X_{GlcNAc} ^{1,5}X_{Man}Y Y^{1,5}X_{Man}
778.31 1.4
781.21 2.3
783.02 1.5

Annotations:

^{2,5}X_{NeuAc}
783.61 6.3

Annotations:

^{2,5}X_{NeuAc}
784.41 250.4

Annotations:

C Y
784.98 19.7
786.36 0.9
787.94 1.2
788.93 1.2

Annotations:

B^{2,5}X_{GlcNAc}
789.69 1.8

Annotations:

B^{2,5}X_{GlcNAc}
790.57 1.6

Annotations:

^{0,3}X_{NeuAc}
792.88 2.5

Annotations:

C^{1,3}X_{NeuAc}
793.38 1.4

Annotations:

C^{1,3}X_{NeuAc}
794.53 3.1
796.01 5.0
796.69 1.4
797.49 2.0
798.55 1.4

Annotations:

^{3,5}X_{NeuAc} ^{1,5}X_{GlcNAc}
801.19 6.7
802.09 0.5
802.61 0.5
805.03 9.5
806.47 1.7
817.56 0.8

Annotations:

^{2,5}A_{Man}Z
818.19 1.0
819.35 77.1

Annotations:

B
820.3 18.1

Annotations:

^{1,4}X_{GlcNAc}
821.1 0.7

Annotations:

C^{1,4}X_{Man} C^{0,3}X_{GlcNAc} Y^{1,4}X_{Man} ^{1,4}X_{GlcNAc}Y Y^{1,4}X_{GlcNAc} ^{1,4}X_{Man}Y
822.03 0.8
825.41 0.7

Annotations:

^{0,4}X_{NeuAc} ^{0,2}X_{GlcNAc}
827.32 0.7
829.7 0.7
836.13 6.3
837.49 1.5

Annotations:

C
840.01 1.9
842.34 2.5
846.04 0.5
850.7 2.0
851.36 1.0

Annotations:

C^{1,3}X_{Man} ^{0,2}A_{Man}Y C^{2,4}X_{GlcNAc} Y^{1,3}X_{Man} Y^{1,3}X_{GlcNAc} ^{1,3}X_{Man}Y
852.44 2.7
853.51 2.0
855.47 0.7

Annotations:

^{0,4}X_{GlcNAc} ^{2,4}X_{GlcNAc}
858.57 1.0
860.44 109.9

Annotations:

^{3,5}X_{GlcNAc}Z Z^{3,5}X_{GlcNAc}
861.41 28.8
867.12 0.9
867.96 33.4
868.72 4.1
869.89 1.2
872.0 1.4
873.1 1.2
875.79 5.6

Annotations:

BZ BZ ZZ
877.0 3.8
877.8 3.3
881.96 1.2
885.92 2.4
886.75 3.2
887.5 1.0
891.51 0.9
892.38 4.7

Annotations:

^{2,4}X_{GlcNAc}Y Y^{0,4}X_{GlcNAc} Y^{2,4}X_{GlcNAc}
893.4 337.3

Annotations:

BY CZ BY CZ ZY YZ
894.44 96.0
910.12 30.1
911.41 1204.3

Annotations:

CY CY YY
912.41 285.3
913.3 4.7
917.52 1.5
932.29 2.4
933.32 1.1
934.49 101.0

Annotations:

Z
935.42 33.0

Annotations:

B^{0,2}X_{Gal}
936.47 5.1
950.89 1.1

Annotations:

B^{2,5}X_{Gal}
951.55 7.8

Annotations:

B^{2,5}X_{Gal}
952.43 229.3

Annotations:

Y
953.51 59.4

Annotations:

C^{0,2}X_{Gal}
954.21 0.5
964.6 1.4
981.51 8.3

Annotations:

BY CZ
982.43 1.6
1001.86 2.3
1002.99 0.8
1004.34 1.3
1021.22 3.5
1022.46 134.8

Annotations:

B^{3,5}X_{GlcNAc} Z^{3,5}X_{GlcNAc} ^{3,5}X_{GlcNAc}Z
1023.49 27.5
1039.83 1.6
1040.45 24.4

Annotations:

C^{3,5}X_{GlcNAc} Y^{3,5}X_{GlcNAc} ^{3,5}X_{GlcNAc}Y
1041.55 5.9
1042.44 2.5
1053.85 6.0

Annotations:

B^{1,4}X_{Man}
1054.77 1.6

Annotations:

^{1,3}X_{Man} C^{2,4}X_{GlcNAc} Y^{1,3}X_{Man} ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y Y^{0,4}X_{GlcNAc} Y^{2,4}X_{GlcNAc} ^{2,4}X_{GlcNAc}Y
1055.47 150.8

Annotations:

BY CZ
1056.49 50.9
1057.59 4.7
1063.37 0.7
1073.49 17.2

Annotations:

CY
1074.44 3.7
1078.48 3.7

Annotations:

ZZ BZ ZZ
1079.48 2.4
1094.32 1.0
1095.37 7.7
1096.49 792.8

Annotations:

Z
1097.49 224.5

Annotations:

^{1,5}A_{Man}Z
1098.54 1.2
1111.49 3.8
1112.09 2.3
1112.94 64.3
1113.7 56.9
1114.45 2514.5

Annotations:

Y
1115.48 677.4

Annotations:

^{1,5}A_{Man}Y
1116.38 41.0
1116.99 2.0
1136.6 3.1
1142.07 1.2

Annotations:

Y^{1,5}X_{Man} ^{1,5}X_{GlcNAc} Y^{1,5}X_{GlcNAc} C^{1,5}X_{Gal} ^{1,5}X_{Gal}Y
1142.95 1.2

Annotations:

BY CZ
1143.59 4.7

Annotations:

BY CZ
1144.69 2.3
1155.38 1.1

Annotations:

B^{0,3}X_{NeuAc}
1166.46 3.0

Annotations:

BZ
1175.81 1.2
1182.87 1.7

Annotations:

^{2,5}X_{NeuAc}Z
1183.55 2.3
1184.53 115.6

Annotations:

BY CZ
1185.5 39.1
1186.5 7.7

Annotations:

^{1,5}X_{NeuAc}Y C^{1,4}X_{Gal} Y^{1,4}X_{Man} ^{1,4}X_{GlcNAc} Y^{1,4}X_{GlcNAc} ^{1,4}X_{Gal}Y
1189.48 1.2

Annotations:

C^{3,5}X_{NeuAc} C^{1,5}X_{GlcNAc}
1202.52 78.4

Annotations:

CY
1203.53 47.3
1204.57 1.6
1205.11 1.1
1225.56 5.8

Annotations:

Z^{3,5}X_{GlcNAc}
1226.55 1.8

Annotations:

B^{0,4}X_{NeuAc} B^{0,2}X_{GlcNAc}
1239.52 1.5

Annotations:

Z^{0,4}X_{GlcNAc} Z^{2,4}X_{GlcNAc}
1240.71 0.8

Annotations:

BZ ZZ
1243.46 19.0

Annotations:

^{3,5}X_{GlcNAc} Y^{3,5}X_{GlcNAc}
1244.7 3.2

Annotations:

C^{0,4}X_{NeuAc} C^{0,2}X_{GlcNAc}
1257.51 3.3

Annotations:

^{2,4}X_{GlcNAc} Y^{0,4}X_{GlcNAc} Y^{2,4}X_{GlcNAc}
1258.56 296.9

Annotations:

BY CZ ZY YZ
1259.57 102.1
1260.39 4.1

Annotations:

C^{2,5}X_{GlcNAc}
1275.27 30.6
1276.52 924.8

Annotations:

CY YY
1277.55 342.8
1278.65 6.8
1280.51 2.3
1299.7 10.7

Annotations:

Z
1300.57 6.4
1315.63 0.5
1316.49 1.1
1317.59 104.5

Annotations:

Y
1318.62 44.6

Annotations:

^{1,5}A_{Man}
1319.66 5.6
1328.51 4.4

Annotations:

BZ
1329.81 0.7
1344.31 2.4

Annotations:

B^{2,5}X_{NeuAc} ^{2,5}X_{NeuAc}Z
1345.04 1.4

Annotations:

^{1,5}X_{Gal}
1346.57 228.1

Annotations:

B
1347.59 82.7
1348.54 9.2

Annotations:

C^{1,5}X_{NeuAc} ^{1,5}X_{NeuAc}Y Y^{1,4}X_{GlcNAc}
1364.61 21.8

Annotations:

C
1365.73 9.2
1366.57 2.5
1369.63 3.1
1370.69 4.3
1380.06 1.9
1383.43 1.3
1385.45 4.9
1386.66 7.0
1387.58 299.0

Annotations:

Z
1388.6 116.3
1389.75 7.4

Annotations:

^{1,4}X_{Gal} Y^{0,3}X_{GlcNAc}
1402.9 5.2
1403.82 9.4
1404.33 61.9
1404.87 120.2
1405.6 2019.6

Annotations:

Y
1406.61 797.5
1407.65 41.9
1408.38 6.3
1409.82 0.5
1460.0 0.7

Annotations:

B^{0,3}X_{GlcNAc}
1461.07 6.3

Annotations:

Z
1461.63 71.7

Annotations:

Z
1462.66 35.3
1463.64 4.7

Annotations:

C^{2,5}X_{GlcNAc}
1476.72 0.9
1477.66 6.7

Annotations:

C^{0,3}X_{GlcNAc} ^{1,4}X_{Man}
1478.34 29.1
1478.96 8.9
1479.62 660.8

Annotations:

Y
1480.64 277.4
1481.65 21.2
1483.08 1.0
1513.79 1.6
1531.58 5.1
1532.69 4.3
1533.63 0.9
1546.82 1.3
1547.62 8.1
1548.81 64.4
1549.66 1037.7

Annotations:

B Z
1550.67 511.8
1551.53 33.0

Annotations:

^{1,5}X_{NeuAc}
1552.21 2.3
1561.8 0.8
1565.35 24.8

Annotations:

^{2,5}X_{NeuAc}
1565.94 69.3

Annotations:

^{2,5}X_{NeuAc}
1566.83 305.7
1567.59 6433.5

Annotations:

C Y
1568.63 3135.4
1569.63 257.7
1570.4 8.2
1571.17 1.2
1571.75 1.4

There are 3 similar spectra:

Id Structure Score

2746

0.95

2762

0.76

2711

0.59
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