Efficient Chemoenzymatic Synthesis of an N-glycan Isomer Library

PubMed Entry

Peak Intensity
336.09 0.3

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Z^{1,3}X_{Man} ^{1,3}X_{Man}Z
366.16 96.7

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BY CZ BY CZ ZY YZ ZY YZ
367.18 21.6

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C^{2,5}X_{Fuc} ^{1,3}A_{Man}Y ^{2,4}A_{Man}Y
368.16 1.9

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CY
383.01 0.2

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C^{1,5}X_{NeuAc} ^{1,4}A_{Man}Y
389.07 0.8

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C^{0,4}X_{Fuc} C^{1,3}X_{NeuAc} ^{1,5}X_{GlcNAc}Y Y^{1,5}X_{GlcNAc} ^{1,5}X_{Man}Y Y^{1,5}X_{Man}
407.18 0.3

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Z
408.13 0.8

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^{0,2}X_{GlcNAc}Z Z^{0,2}X_{GlcNAc} ^{1,3}A_{Man}Z B^{2,5}X_{Gal} ^{0,4}A_{Man}Z B^{0,2}X_{Gal}
419.23 0.2

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C
437.03 0.3
454.03 7.4

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B
454.6 0.6

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B
455.8 1.1

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YY
477.23 0.2
481.36 2.3

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Z^{1,5}X_{Man} ^{1,5}X_{Gal}Z
495.06 0.3
496.2 0.8

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^{2,5}X_{Gal}Z Z^{2,5}X_{GlcNAc} ^{2,5}X_{GlcNAc}Z
512.18 31.2

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BY CZ BY CZ
513.2 2.5

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514.13 2.5

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^{2,4}A_{GlcNAc} C^{1,5}X_{Man}
526.94 3.7

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B^{1,4}X_{Man}
527.62 0.6

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B^{1,4}X_{Man} Y^{1,3}X_{Man} ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y Y^{2,4}X_{GlcNAc} ^{2,4}X_{GlcNAc}Y
528.22 16.5

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BY CZ
529.22 2.8
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CY CY
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YY
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ZZ
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565.59 0.8
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ZY YZ
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B^{0,2}X_{NeuAc}
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BZ
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600.14 0.4

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Z^{1,3}X_{Man} ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z B^{1,5}X_{NeuAc} C^{0,2}X_{NeuAc} ^{0,3}A_{Man}Z B^{1,4}X_{Man}
601.38 0.8

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CY
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611.24 1.1
618.11 2.2

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C^{1,5}X_{NeuAc} ^{0,3}A_{Man}Y
627.28 0.4
630.2 1.0

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ZY YZ
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C^{1,4}X_{Man}
639.19 0.7

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YY BZ
640.32 0.5
641.33 4.0

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Z^{1,4}X_{Man} ^{1,4}X_{Man}Z
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Y^{0,2}X_{Gal}
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BZ BZ
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B
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659.15 10.7

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B^{1,5}X_{GlcNAc} Y^{1,4}X_{Man} ^{1,4}X_{Man}Y
673.11 1.1

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674.35 7.0

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CZ BY CY
675.21 0.3

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C
684.01 0.6
690.21 3.2

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C^{1,4}X_{GlcNAc}
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696.32 1.3

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C^{1,5}X_{GlcNAc} ^{3,5}X_{NeuAc}Y
698.22 0.2
702.1 4.7

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B^{1,4}X_{Man} B^{0,3}X_{GlcNAc} B^{2,4}X_{NeuAc} ^{2,4}X_{GlcNAc}Y
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YY
713.35 0.9

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ZZ ZZ
730.14 1.2

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Z^{0,3}X_{Fuc} ^{1,4}X_{Man}Z
731.36 25.1

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YZ ZY YZ ZY
732.31 9.2

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YY
733.28 0.3

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C^{2,5}X_{Fuc} ^{2,4}X_{NeuAc}Y
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746.82 0.8

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CZ CZ CZ BY BY BY
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YY YY
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Y^{0,4}X_{Fuc} ^{1,3}X_{Man}Y Y^{1,3}X_{NeuAc} C^{2,4}X_{GlcNAc} ^{1,3}X_{Man} Y^{2,5}X_{NeuAc}
782.54 0.5

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B^{1,4}X_{GlcNAc} B^{1,4}X_{Gal} B^{1,4}X_{Gal}
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Y^{1,3}X_{Gal} Y^{2,4}X_{Gal} ^{2,4}X_{GlcNAc}Y ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y
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796.38 0.3

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B^{2,5}X_{GlcNAc} Z^{0,2}X_{Fuc}
797.44 0.9

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C^{0,2}X_{GlcNAc} Y^{0,4}X_{NeuAc} ^{0,2}A_{Man}Z ^{0,2}A_{Man}Z B^{1,3}X_{Gal} B^{2,4}X_{Gal} Z^{2,4}X_{NeuAc} B^{1,3}X_{Gal} B^{2,4}X_{Gal}
801.25 0.8

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BZ
802.44 3.0
803.32 336.6

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B
804.27 95.8

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YZ YZ ZY ZY
805.43 9.8

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C^{2,5}X_{GlcNAc} Y^{0,2}X_{Fuc}
806.5 1.6

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^{0,2}A_{Man}Y C^{1,3}X_{Gal} C^{2,4}X_{Gal} Y^{2,4}X_{NeuAc} ^{0,2}A_{Man}Y C^{1,3}X_{Gal} C^{2,4}X_{Gal}
813.85 3.2

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^{1,5}A_{Man}Y ^{1,5}A_{Man}Y ^{0,2}X_{GlcNAc}Z Z^{0,2}X_{GlcNAc}
815.11 0.7
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B
820.47 1.6

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C^{3,5}X_{GlcNAc} Y^{0,3}X_{Fuc} ^{1,4}X_{GlcNAc}Y ^{1,4}X_{GlcNAc}
821.21 1.3

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C
824.76 0.3

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^{2,5}X_{GlcNAc}Z
835.97 0.9
836.84 1.9

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C
837.35 0.8

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C
842.46 0.2

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Y^{2,5}X_{Gal} ^{2,5}X_{Gal}Y
853.18 1.0
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Y
859.59 1.7
860.37 5.0

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^{3,5}X_{GlcNAc}Z
861.44 1.3

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^{1,3}A_{Man}Z ^{2,4}A_{Man}Z B^{0,2}X_{Fuc}
877.39 7.3

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B^{2,5}X_{Fuc} B^{2,5}X_{Gal} B^{2,5}X_{Gal} ^{1,5}A_{Man}Z B^{0,3}X_{NeuAc} B^{0,3}X_{NeuAc}
878.95 10.8

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885.88 1.1

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YY YY
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Z^{0,4}X_{NeuAc} ^{0,4}X_{NeuAc}Z
891.67 0.4

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BZ
892.82 5.0

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893.39 15.7

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ZY YZ
894.26 16.2

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YY
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C^{3,5}X_{NeuAc} C^{3,5}X_{NeuAc} C^{2,5}X_{Fuc}
896.52 3.0
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^{0,2}X_{GlcNAc} Y^{0,4}X_{NeuAc} ^{0,4}X_{NeuAc}Y B^{1,3}X_{Gal} B^{2,4}X_{Gal} Z^{2,4}X_{NeuAc} ^{2,4}X_{NeuAc}Z B^{1,3}X_{Gal} B^{2,4}X_{Gal}
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Z^{1,4}X_{Fuc} ^{1,4}X_{Fuc}Z
909.72 5.1

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CY
910.23 1.7
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YY
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Z^{0,3}X_{Fuc} ^{0,3}X_{Fuc}Z ^{1,4}X_{Gal}Z Z^{1,4}X_{Gal}
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C^{0,4}X_{NeuAc} C^{0,4}X_{NeuAc} B^{2,4}X_{NeuAc} B^{2,4}X_{NeuAc}
929.34 2.1

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CZ CZ BY BY
930.57 2.9

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C^{2,4}X_{NeuAc} C^{2,4}X_{NeuAc}
933.69 0.9
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ZY YZ
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Z^{0,3}X_{NeuAc} ^{0,3}X_{NeuAc}Z
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B^{0,3}X_{Fuc}
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B^{3,5}X_{Fuc} ^{2,5}A_{Man} Y^{0,3}X_{NeuAc} ^{0,3}X_{NeuAc}Y
948.47 18.4

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ZZ ZZ
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B^{0,4}X_{Fuc} B^{1,3}X_{NeuAc} B^{1,3}X_{NeuAc}
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Y Y
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Z^{1,5}X_{Man} ^{1,5}X_{Gal}Z
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BZ
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B^{0,2}X_{NeuAc} B^{0,2}X_{NeuAc}
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B
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YY YY
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C^{1,5}X_{NeuAc} C^{1,5}X_{NeuAc}
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CZ BY
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C
989.45 0.8

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Y^{2,4}X_{NeuAc} ^{2,4}X_{NeuAc}Y
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998.41 1.3
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CY
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^{2,5}X_{NeuAc}Z Z^{2,5}X_{NeuAc} Z^{1,3}X_{Fuc} ^{1,3}X_{Fuc}Z Z^{2,4}X_{Fuc} ^{2,4}X_{Fuc}Z
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^{2,4}X_{Gal} B^{1,5}X_{Man} ^{2,5}X_{NeuAc}Y Y^{2,5}X_{NeuAc} Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} ^{1,3}X_{Gal} ^{2,4}X_{Gal} ^{1,3}X_{Fuc}Y ^{2,4}X_{Fuc}Y ^{1,3}X_{Gal}
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Y^{0,4}X_{Fuc} Y^{1,3}X_{NeuAc} ^{0,4}X_{Fuc}Y ^{1,3}X_{NeuAc}Y
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B^{0,2}X_{Fuc}
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C^{1,4}X_{NeuAc} C^{1,4}X_{NeuAc}
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Z Z
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C^{0,2}X_{Fuc}
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Y Y
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B^{3,5}X_{Fuc} ^{1,4}X_{NeuAc}Z Z^{1,4}X_{NeuAc}
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^{2,5}A_{Man}Z
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B
1075.81 1.2

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YZ ZY YZ ZY
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YY
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1103.72 1.2

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Z
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1105.21 1.4
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Z^{1,5}X_{Gal} ^{1,5}X_{Man}Z
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1111.43 1.0
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Y
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YY YY
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^{1,5}A_{Man}Y
1115.94 1.0
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1119.55 0.5
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BZ
1126.4 1.2

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1127.45 8.0

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CZ BY
1129.05 0.5
1136.79 3.3
1137.77 0.2
1141.31 0.3

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1142.52 1.3

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Y^{1,5}X_{Man} Y^{1,5}X_{GlcNAc} ^{1,5}X_{Gal}Y
1143.36 2.8

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BY CZ
1146.55 1.6
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1149.89 16.6
1150.9 5.2
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CY
1162.85 0.7
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^{1,5}X_{NeuAc}Z Z^{1,4}X_{Man} Z^{1,4}X_{GlcNAc} ^{1,4}X_{Gal}Z
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^{2,5}X_{Gal}Y
1173.41 0.3

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C^{1,5}X_{Man}
1177.88 1.2
1185.42 2.7
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^{1,5}X_{NeuAc}Y Y^{1,4}X_{Man} Y^{1,4}X_{GlcNAc} ^{1,4}X_{Gal}Y
1187.44 1.3
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Z^{1,3}X_{Man} ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z
1201.35 14.7
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CY
1203.49 0.4
1207.44 5.9
1218.48 4.1
1225.38 2.0
1242.4 6.4

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YZ YZ ZY ZY
1243.41 2.7

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^{1,5}A_{Man}Z
1244.08 0.5

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C^{0,2}X_{GlcNAc}
1254.33 0.4
1256.43 13.7

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B^{1,4}X_{Man}
1257.57 2.3

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Y^{2,4}X_{GlcNAc}
1258.59 28.6

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YZ ZY
1259.43 30.9
1260.46 23.5

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YY YY
1261.37 11.1

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^{1,5}A_{Man}Y
1272.44 1.5

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^{1,4}X_{NeuAc}Z
1274.39 6.1

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C^{1,4}X_{Man}
1275.02 2.5
1275.96 4.4
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YY
1277.42 42.0
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CZ BY
1296.42 0.3
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Y^{1,5}X_{GlcNAc} C^{1,3}X_{Man} C^{2,4}X_{GlcNAc} ^{2,4}X_{NeuAc}Y
1313.71 1.7
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Z^{1,5}X_{NeuAc} ^{1,4}X_{Man}Z Z^{1,4}X_{Gal} ^{1,4}X_{GlcNAc}Z
1322.18 1.5
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CZ BY
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Y^{1,5}X_{NeuAc} ^{1,4}X_{Man}Y Y^{1,4}X_{Gal} ^{1,4}X_{GlcNAc}Y
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BY CZ
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CY
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^{2,5}X_{NeuAc}Y Y^{1,3}X_{Gal} Y^{2,4}X_{Gal} ^{1,3}X_{Man}Y
1364.24 1.1

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CY
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1366.61 3.3
1379.38 1.2

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C^{1,4}X_{GlcNAc}
1383.31 1.7
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ZZ
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Z
1388.44 29.2

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B^{2,5}X_{GlcNAc}
1389.5 16.4

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^{3,5}X_{GlcNAc}Y
1395.55 0.9
1403.23 0.9

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^{2,4}X_{GlcNAc}Y
1404.64 48.2

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YZ ZY
1405.53 116.2

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Y
1406.5 90.6

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C^{2,5}X_{GlcNAc}
1407.18 7.9
1407.78 11.8
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C^{1,4}X_{Man} C^{0,3}X_{GlcNAc}
1421.66 3.8
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YY
1423.55 53.7
1424.53 22.5
1425.57 8.3
1438.26 0.3
1439.63 0.3
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YZ ZY
1456.08 2.7
1459.41 2.0

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Z^{0,3}X_{Fuc} ^{1,4}X_{Man}Z
1460.56 1.9
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YY
1476.54 2.9

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^{1,4}X_{GlcNAc}Z Z^{1,5}X_{NeuAc}
1477.82 4.7

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Y^{0,3}X_{Fuc} ^{1,4}X_{Man}Y ^{1,4}X_{Man}
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1492.64 19.0

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CZ CZ CZ BY BY BY
1493.63 19.5

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Y^{3,5}X_{Fuc}
1494.65 5.3

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Y^{1,5}X_{NeuAc} ^{1,4}X_{GlcNAc}Y
1510.45 6.9

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CY CY CY
1511.43 2.8
1512.13 0.8
1525.96 0.6
1532.96 5.6
1533.5 26.8

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Z
1534.57 28.8

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B^{0,2}X_{Gal} B^{0,2}X_{Gal}
1538.81 0.7

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C^{1,5}X_{GlcNAc} C^{1,5}X_{Gal} C^{1,5}X_{Gal} Y^{3,5}X_{NeuAc}
1548.54 3.3
1549.58 86.0

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Z
1550.66 113.2

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B^{2,5}X_{Gal} B^{2,5}X_{Gal}
1551.65 76.5

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Y
1552.54 58.0

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C^{0,2}X_{Gal} C^{0,2}X_{Gal}
1553.4 13.8
1565.98 5.3

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Y^{1,3}X_{Gal} Y^{2,4}X_{Gal} ^{2,4}X_{GlcNAc}Y ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y
1566.75 1.9
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Y
1568.56 321.9

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C^{2,5}X_{Gal} C^{2,5}X_{Gal}
1569.48 71.3
1570.11 0.7
1574.49 1.1
1584.42 0.6
1586.59 6.8
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1606.67 1.5
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^{1,5}A_{Man}Z ^{1,5}A_{Man}Z
1616.92 0.8
1621.0 1.1
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B^{3,5}X_{GlcNAc} Z^{0,3}X_{Fuc} ^{1,4}X_{GlcNAc}Z
1622.51 13.6
1623.67 0.8

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C^{0,3}X_{GlcNAc} Y^{1,4}X_{Fuc} ^{1,4}X_{Man}
1625.41 0.8

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YY YY
1626.59 2.4

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^{1,5}A_{Man}Y ^{1,5}A_{Man}Y
1629.07 0.7
1638.28 2.9
1642.52 8.9
1653.07 1.1
1655.41 0.9

Annotations:

Y^{3,5}X_{Fuc}
1668.07 3.8

Annotations:

Y^{0,2}X_{Gal} ^{0,2}X_{Gal}Y
1671.3 4.2
1674.21 0.7
1678.49 1.3

Annotations:

^{3,5}X_{GlcNAc}Z
1679.57 1.2

Annotations:

Z^{1,5}X_{NeuAc} ^{1,5}X_{NeuAc}Z ^{1,4}X_{Gal}Z Z^{1,4}X_{Gal}
1694.51 1.0
1695.56 103.4

Annotations:

Z
1696.55 88.7

Annotations:

^{3,5}X_{GlcNAc}Y
1697.55 15.7

Annotations:

Y^{1,5}X_{NeuAc} ^{1,5}X_{NeuAc}Y ^{1,4}X_{Gal}Y Y^{1,4}X_{Gal}
1698.53 2.4
1711.58 4.4

Annotations:

Y^{2,5}X_{NeuAc} ^{2,5}X_{NeuAc}Y
1712.38 36.9
1713.59 293.1

Annotations:

Y
1714.58 304.3
1715.64 70.2
1716.5 1.9
1729.07 0.3
1730.88 1.8
1754.07 2.3

Annotations:

B^{2,5}X_{Gal} B^{2,5}X_{Gal} ^{1,5}A_{Man}Z
1769.4 3.8

Annotations:

YZ ZY
1770.73 3.0

Annotations:

YY YY
1771.55 5.6

Annotations:

C^{2,5}X_{Gal} C^{2,5}X_{Gal} ^{1,5}A_{Man}Y
1784.61 5.2
1786.77 0.8
1787.58 6.9

Annotations:

YY
1788.11 0.9

Annotations:

YY
1788.67 3.2

Annotations:

C^{3,5}X_{NeuAc} C^{3,5}X_{NeuAc}
1835.25 2.0
1841.84 0.8

Annotations:

B^{0,2}X_{Fuc} Z^{1,5}X_{NeuAc} ^{1,5}X_{NeuAc}Z
1842.65 3.2

Annotations:

^{1,4}X_{Gal}Y Y^{1,4}X_{Gal} Y^{0,3}X_{Fuc} ^{3,5}X_{GlcNAc} ^{0,3}X_{Fuc}Y
1858.64 4.4

Annotations:

Y^{3,5}X_{Fuc} ^{3,5}X_{Fuc}Y
1859.58 8.0

Annotations:

C^{0,2}X_{Fuc} Y^{1,5}X_{NeuAc} ^{1,5}X_{NeuAc}Y
1860.67 6.5
1875.4 2.0

Annotations:

CY CY
1876.44 2.1
1881.93 0.5
1898.78 0.8

Annotations:

Z Z
1916.53 9.4

Annotations:

Y Y
1917.74 4.7

Annotations:

^{1,5}A_{Man}
1918.72 1.3
1930.92 0.3
1932.68 13.1

Annotations:

YY YY
1933.77 36.1
1934.55 4.5
1946.52 4.5
1947.7 2.2

Annotations:

C^{1,5}X_{NeuAc} C^{1,5}X_{NeuAc}
1969.74 0.4
1986.66 6.6

Annotations:

^{1,5}X_{NeuAc}Z Z^{1,5}X_{NeuAc} Z^{0,3}X_{Fuc} ^{0,3}X_{Fuc}Z
1987.48 2.6

There are 4 similar spectra:

Id Structure Score

2760

0.96

2756

0.92

2759

0.79

2710

0.79
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