Efficient Chemoenzymatic Synthesis of an N-glycan Isomer Library

PubMed Entry

Peak Intensity
273.35 0.6

Annotations:

CY
274.15 40.4

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^{0,4}A_{Man}
275.07 1.6
291.61 1.5

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B
292.13 47.0

Annotations:

B
293.04 2.2

Annotations:

^{1,4}A_{GlcNAc}
296.75 0.3

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C^{3,5}X_{NeuAc}
304.99 0.5
310.11 1.1

Annotations:

C
325.22 2.8

Annotations:

BY BY CZ CZ BY CZ
329.42 0.3

Annotations:

B
330.13 0.7

Annotations:

^{1,4}X_{Man}Y
342.6 0.6
364.37 0.5
365.37 0.4
366.2 284.8

Annotations:

BY CZ CZ YZ BY ZY
367.22 14.8

Annotations:

B^{0,2}X_{Man} ^{0,4}A_{Man}Z ^{0,4}A_{Man}Z B^{0,2}X_{Man}
384.09 0.3

Annotations:

CY CY
388.15 0.5

Annotations:

B^{1,3}X_{NeuAc}
400.18 0.2
400.82 0.4

Annotations:

C^{2,5}X_{Man} C^{2,5}X_{Man}
407.19 0.5

Annotations:

Z
416.07 0.2

Annotations:

^{2,5}X_{GlcNAc}Z
418.13 1.2

Annotations:

BZ
424.61 0.6

Annotations:

B^{2,5}X_{Gal} ^{2,5}X_{GlcNAc}Y
425.27 5.5

Annotations:

Y
436.22 0.7

Annotations:

B^{0,2}X_{NeuAc}
447.19 6.4

Annotations:

YZ YZ ZY ZY
447.84 0.4

Annotations:

B^{0,2}X_{GlcNAc}
452.96 1.3

Annotations:

^{1,5}X_{Man}
454.2 27.2

Annotations:

B
455.09 1.7

Annotations:

^{1,4}A_{Man}
456.27 1.1

Annotations:

YY YY
467.67 1.4

Annotations:

YZ ZY
472.26 0.5

Annotations:

C
473.11 0.4
476.7 3.4

Annotations:

YY
485.43 0.8

Annotations:

Y^{2,5}X_{Man} Y^{2,5}X_{Man}
487.29 1.4

Annotations:

B
494.89 0.7
495.43 1.0
496.19 0.8

Annotations:

Z^{2,5}X_{Man} ^{2,5}X_{Gal}Z
499.66 0.2
505.11 0.4

Annotations:

C
506.26 0.1
510.31 0.2

Annotations:

BZ
526.55 1.0
528.23 109.4

Annotations:

Z
529.08 6.3

Annotations:

B^{0,2}X_{Gal} C^{2,5}X_{Man}
530.25 0.4

Annotations:

C^{1,5}X_{GlcNAc} C^{1,5}X_{Gal}
536.17 0.2
545.26 0.3

Annotations:

C^{2,5}X_{Gal}
546.25 0.5

Annotations:

CY
548.82 5.9

Annotations:

YZ ZY
556.65 0.2

Annotations:

B^{2,4}X_{NeuAc}
557.86 15.6

Annotations:

YY
569.29 2.2

Annotations:

YZ ZY
570.47 0.4

Annotations:

^{1,3}A_{Man}Z ^{2,4}A_{Man}Z
577.24 0.3

Annotations:

Z^{2,5}X_{Man} ^{2,5}X_{GlcNAc}Z ^{2,5}X_{GlcNAc}Z ^{0,3}A_{Man}
582.04 0.3
585.87 0.3
587.29 22.0

Annotations:

YY
588.15 1.6

Annotations:

^{1,3}A_{Man}Y ^{2,4}A_{Man}Y C^{2,4}X_{GlcNAc} Y^{0,4}X_{Man} Y^{1,3}X_{Man} Y^{2,4}X_{Man} Y^{0,4}X_{Man} Y^{1,3}X_{Man} Y^{2,4}X_{Man} ^{1,3}X_{Man}
592.74 0.8

Annotations:

BY CZ
600.91 1.1

Annotations:

^{2,5}X_{NeuAc}Y
606.49 0.4

Annotations:

B^{1,5}X_{Man} ^{3,5}X_{NeuAc}Z
609.29 4.8

Annotations:

Z
616.34 3.1

Annotations:

C^{0,2}X_{NeuAc}
617.33 0.5
618.31 0.3

Annotations:

Y
619.55 0.3
622.16 0.2

Annotations:

B^{2,5}X_{Man}
629.84 9.4

Annotations:

YZ YZ ZY ZY
630.5 1.0

Annotations:

B^{0,2}X_{Gal} ^{1,5}A_{Man}Z ^{1,5}A_{Man}Z C^{2,5}X_{Man}
639.02 34.6

Annotations:

YY YY
639.61 0.8

Annotations:

C^{0,2}X_{Gal} ^{1,5}A_{Man}Y ^{1,5}A_{Man}Y
641.5 0.5

Annotations:

^{1,4}X_{Man}Z
648.51 0.7
649.27 0.5

Annotations:

BY CZ
654.33 0.7

Annotations:

C^{1,4}X_{Gal} ^{1,4}X_{GlcNAc}
655.22 1.0

Annotations:

C^{3,5}X_{NeuAc}
656.38 8.5
657.28 971.1

Annotations:

B
658.32 65.1

Annotations:

^{2,5}X_{Gal}Z ^{2,5}X_{Gal}Z Z^{2,5}X_{Man} Z^{2,5}X_{Man}
659.3 0.2

Annotations:

^{1,4}X_{Man}Y Y^{0,2}X_{Man} Y^{0,2}X_{Man} ^{0,2}X_{GlcNAc} ^{0,2}X_{Gal}Y ^{0,2}X_{Gal}Y
664.65 0.7
667.15 0.3

Annotations:

CY
671.14 0.3

Annotations:

^{1,3}X_{Man}Z
673.83 3.9

Annotations:

Z^{3,5}X_{Man} Z^{3,5}X_{Man} B^{0,2}X_{Man} B^{0,2}X_{Man} B^{0,4}X_{NeuAc}
687.96 0.5
688.8 0.4

Annotations:

B^{0,3}X_{Man} B^{1,4}X_{Man} B^{0,3}X_{Man} B^{1,4}X_{Man} ^{1,3}X_{Man}Y
689.3 3.3

Annotations:

Y^{1,3}X_{Man} Y^{2,4}X_{Man} Y^{0,4}X_{Man} Y^{1,3}X_{Man} Y^{2,4}X_{Man} ^{2,4}X_{GlcNAc} B^{0,3}X_{Man} B^{1,4}X_{Man} B^{0,3}X_{Man} B^{1,4}X_{Man} ^{1,3}X_{Man}Y ^{1,3}X_{Gal}Y ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y ^{2,4}X_{Gal}Y Y^{0,4}X_{Man}
690.3 145.9

Annotations:

BY CZ
691.23 7.8

Annotations:

C^{2,5}X_{Man} C^{2,5}X_{Man}
694.35 0.6

Annotations:

Z
699.83 0.6
701.9 0.2

Annotations:

ZZ ZZ
703.32 1.4

Annotations:

Y
703.91 0.7

Annotations:

B^{1,4}X_{NeuAc} B^{0,4}X_{Man} B^{1,3}X_{Man} B^{2,4}X_{Man} B^{0,4}X_{Man} B^{1,3}X_{Man} B^{2,4}X_{Man}
708.33 1.4
710.82 12.8

Annotations:

Z
716.46 0.3
718.11 0.4

Annotations:

B^{2,4}X_{NeuAc}
718.98 1.8
719.9 43.6

Annotations:

Y
720.43 2.0

Annotations:

^{1,5}A_{Man}
721.01 0.3

Annotations:

B^{1,4}X_{Man} C^{1,3}X_{NeuAc}
725.19 0.7

Annotations:

Z^{1,5}X_{Man} Z^{1,5}X_{Man} B^{0,2}X_{NeuAc}
729.33 0.3
730.48 0.4
731.38 22.1

Annotations:

YZ ZY
732.01 1.0

Annotations:

Z^{0,2}X_{Man} Z^{0,2}X_{Man} ^{2,5}X_{Man}
732.6 1.5

Annotations:

^{2,5}X_{Man}
745.89 0.4

Annotations:

BZ BZ
746.75 0.1

Annotations:

^{1,5}X_{NeuAc}Z ^{1,5}X_{NeuAc}Z Z^{0,3}X_{Man} Z^{1,4}X_{Man} Z^{0,3}X_{Man} Z^{1,4}X_{Man}
747.58 0.5

Annotations:

^{1,4}A_{Man}
748.34 3.1

Annotations:

B^{3,5}X_{NeuAc} Y^{2,5}X_{Man} Y^{2,5}X_{Man} ^{2,5}X_{Gal}
749.35 104.9

Annotations:

YY
750.37 5.1
755.11 3.4

Annotations:

BY BY CZ CZ
765.35 0.7

Annotations:

^{1,5}A_{Man}Z
766.32 1.3

Annotations:

ZZ
775.33 4.7

Annotations:

YZ ZY
775.92 0.5

Annotations:

B^{0,2}X_{Man} B^{0,2}X_{Man} B^{0,4}X_{NeuAc}
778.15 1.0

Annotations:

C^{1,5}X_{Man} C^{1,5}X_{Man} ^{3,5}X_{NeuAc}Y ^{3,5}X_{NeuAc}Y B^{1,4}X_{NeuAc}
783.23 0.3

Annotations:

^{1,5}A_{Man}Y
784.24 7.7

Annotations:

YY
785.09 1.4

Annotations:

C^{0,2}X_{Man} C^{0,2}X_{Man} C^{0,4}X_{NeuAc} B^{2,4}X_{NeuAc}
792.03 14.0

Annotations:

Z
799.53 1.6

Annotations:

C^{0,3}X_{Man} C^{1,4}X_{Man} C^{0,3}X_{Man} C^{1,4}X_{Man}
800.98 39.0

Annotations:

Y
811.31 0.4

Annotations:

BY CZ
814.38 0.7

Annotations:

^{0,2}X_{GlcNAc}Z C^{0,4}X_{Man} C^{1,3}X_{Man} C^{2,4}X_{Man} C^{0,4}X_{Man} C^{1,3}X_{Man} C^{2,4}X_{Man} ^{2,4}X_{NeuAc}Y ^{2,4}X_{NeuAc}Y
818.53 4.1
819.36 84.4

Annotations:

B
820.34 8.6

Annotations:

^{0,3}X_{Man}Y ^{1,4}X_{Man}Y Y^{0,3}X_{Man} Y^{1,4}X_{Man}
820.97 0.4

Annotations:

^{1,4}X_{GlcNAc}Y
826.52 1.1

Annotations:

^{0,4}X_{Man}Z ^{1,3}X_{Man}Z ^{2,4}X_{Man}Z Z^{0,4}X_{Man} Z^{1,3}X_{Man} Z^{2,4}X_{Man}
829.1 0.2

Annotations:

CY
834.45 0.5

Annotations:

BZ
835.2 1.2

Annotations:

^{1,3}X_{Man}Y ^{2,4}X_{Man}Y Y^{0,4}X_{Man} Y^{1,3}X_{Man} Y^{2,4}X_{Man} ^{0,4}X_{Man}Y
836.05 6.9

Annotations:

B
838.56 0.5
842.35 0.6
843.86 1.2

Annotations:

^{2,5}X_{NeuAc} ^{0,3}X_{GlcNAc}Z
845.04 1.3

Annotations:

C
847.5 0.6
850.98 1.7

Annotations:

^{0,3}X_{NeuAc}
851.66 4.3
852.37 172.2

Annotations:

BY CZ
853.34 15.2
856.27 6.5

Annotations:

Z Z
856.92 5.8

Annotations:

C^{1,5}X_{GlcNAc}
860.44 0.9

Annotations:

^{3,5}X_{GlcNAc}Z
861.17 0.2
865.42 38.1

Annotations:

Y Y
866.14 4.0
870.06 1.7

Annotations:

CY
870.94 0.7
874.35 0.3

Annotations:

^{2,4}X_{GlcNAc}Z
875.44 0.2

Annotations:

ZZ ZZ
878.45 0.3

Annotations:

^{3,5}X_{GlcNAc}Y
879.39 0.2

Annotations:

^{1,3}A_{Man} ^{2,4}A_{Man} ^{1,5}X_{Man} ^{1,5}X_{Man}
892.32 1.4

Annotations:

^{2,4}X_{GlcNAc}Y
893.37 67.0

Annotations:

YZ YZ ZY ZY
894.43 7.0

Annotations:

B^{0,2}X_{GlcNAc} ^{2,5}X_{Man} ^{2,5}X_{Man}
896.24 0.9

Annotations:

^{2,4}X_{NeuAc}
896.85 0.3
908.29 0.2
909.35 0.4

Annotations:

^{3,5}X_{Man} ^{3,5}X_{Man} ^{1,4}A_{Man}
910.14 7.2

Annotations:

B^{2,5}X_{GlcNAc}
911.38 279.9

Annotations:

YY YY
912.37 22.8

Annotations:

C^{0,2}X_{GlcNAc}
928.72 2.5

Annotations:

C^{2,5}X_{GlcNAc}
931.33 0.4
934.4 0.8

Annotations:

ZY YZ
936.54 0.4
938.39 0.4

Annotations:

^{2,5}A_{Man}Z
945.81 0.2
946.32 0.3
947.76 0.5
952.47 2.5

Annotations:

YY
959.33 0.3
981.36 14.8

Annotations:

BY CZ
982.5 1.5
990.93 0.5
1011.68 0.3
1012.43 0.5
1013.37 1.5

Annotations:

^{1,3}X_{Man}Y ^{1,3}X_{Man}Y Y^{0,4}X_{Man} Y^{1,3}X_{Man} Y^{2,4}X_{Man} Y^{0,4}X_{Man} Y^{1,3}X_{Man} Y^{2,4}X_{Man}
1014.42 200.7

Annotations:

BY CZ BY CZ
1015.43 21.5
1016.16 1.1
1019.31 0.4
1020.43 0.5
1021.65 0.4
1031.44 0.3
1032.4 2.3

Annotations:

CY CY
1037.35 0.9

Annotations:

ZZ ZZ
1037.95 0.3
1040.48 0.5
1041.08 0.3

Annotations:

C^{0,2}X_{Man}
1053.67 0.3
1054.64 3.9

Annotations:

^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y
1055.43 179.1

Annotations:

Z
1056.43 22.4

Annotations:

B^{0,2}X_{Gal}
1057.21 0.9

Annotations:

C^{2,5}X_{Man}
1058.41 0.4
1063.42 0.7
1071.23 2.5
1072.08 6.8

Annotations:

B^{2,5}X_{Gal}
1072.67 12.5

Annotations:

B^{2,5}X_{Gal}
1073.41 603.1

Annotations:

Y
1074.41 89.3

Annotations:

C^{0,2}X_{Gal}
1075.08 1.6
1089.81 0.6
1096.49 2.1

Annotations:

YZ ZY
1097.04 0.2

Annotations:

B^{0,2}X_{GlcNAc} Z^{0,2}X_{Man} Z^{0,2}X_{Man}
1102.4 0.5
1112.69 0.5
1114.46 7.6

Annotations:

YY
1115.5 2.5

Annotations:

C^{0,2}X_{GlcNAc} Y^{0,2}X_{Man} Y^{0,2}X_{Man}
1125.54 0.2
1126.54 0.4
1140.43 0.3
1142.63 1.1

Annotations:

Y^{1,5}X_{Man}
1143.48 11.0

Annotations:

B^{3,5}X_{GlcNAc} Z^{3,5}X_{Man} Z^{3,5}X_{Man}
1144.35 3.6
1160.76 0.3
1166.49 0.8

Annotations:

BZ
1176.53 2.9

Annotations:

BY CZ
1177.55 0.3
1184.45 2.2

Annotations:

BY CZ
1185.37 0.3
1199.5 0.9

Annotations:

BZ
1201.54 0.3
1202.54 3.8

Annotations:

CY
1203.55 2.0
1215.35 0.4
1216.42 3.8

Annotations:

^{2,4}X_{GlcNAc}Y ^{2,4}X_{GlcNAc}Y
1217.49 297.2

Annotations:

Z
1218.51 49.2
1232.68 1.3
1233.56 9.4
1234.65 66.4
1235.46 1373.9

Annotations:

Y
1236.48 242.4
1237.17 4.9
1237.7 0.5
1238.31 0.5
1240.61 1.3

Annotations:

ZZ ZZ
1243.02 0.7
1257.74 0.2
1258.52 9.0

Annotations:

YZ YZ ZY ZY
1259.63 1.0

Annotations:

B^{0,2}X_{Gal} ^{1,5}A_{Man}Z ^{1,5}A_{Man}Z
1273.76 0.2
1275.57 3.2

Annotations:

B^{2,5}X_{Gal}
1276.55 28.2

Annotations:

YY YY
1277.6 3.9

Annotations:

C^{0,2}X_{Gal} ^{1,5}A_{Man}Y ^{1,5}A_{Man}Y
1278.17 0.6
1305.5 12.5

Annotations:

BY BY CZ CZ
1306.5 4.4
1321.1 0.2
1328.21 1.0
1328.8 0.3
1344.24 0.8
1345.85 0.5
1346.54 14.2

Annotations:

Z^{3,5}X_{Man} Z^{3,5}X_{Man}
1347.6 1.2

Annotations:

B^{0,2}X_{Man} B^{0,2}X_{Man} B^{0,4}X_{NeuAc}
1361.52 0.3

Annotations:

BZ
1363.65 0.5

Annotations:

B^{2,5}X_{Man} B^{2,5}X_{Man}
1364.47 22.0

Annotations:

Y^{3,5}X_{Man} Y^{3,5}X_{Man} ^{3,5}X_{GlcNAc}
1365.6 4.5

Annotations:

C^{0,2}X_{Man} C^{0,2}X_{Man} C^{0,4}X_{NeuAc}
1379.53 15.7

Annotations:

BY CZ
1380.56 4.2
1396.87 0.6
1397.67 4.7

Annotations:

CY
1398.57 0.3
1399.13 0.4
1400.81 0.7
1406.61 0.3
1419.35 0.4
1420.61 27.3

Annotations:

Z
1421.58 4.6
1436.71 0.4
1437.5 0.4
1438.56 66.1

Annotations:

Y
1439.55 11.5

Annotations:

^{1,5}A_{Man}
1467.49 3.1

Annotations:

B
1468.45 0.6
1485.6 0.6

Annotations:

C
1486.59 0.4
1490.49 0.6

Annotations:

BZ BZ
1491.47 0.3
1501.77 0.3
1502.55 0.4
1505.68 0.2
1507.17 0.4
1507.81 1.3
1508.65 28.9

Annotations:

BY BY CZ CZ
1509.65 9.8
1524.73 0.9

Annotations:

^{2,5}X_{NeuAc}Y ^{2,5}X_{NeuAc}Y
1525.55 0.5
1526.65 31.9

Annotations:

CY CY
1527.67 16.2
1528.81 0.4
1581.64 0.3
1582.58 13.0

Annotations:

Z
1583.56 4.3
1584.38 0.4

Annotations:

C^{2,5}X_{Man} C^{2,5}X_{Man}
1599.74 1.4
1600.68 78.8

Annotations:

Y
1601.65 13.6
1623.79 0.3
1628.54 0.5

Annotations:

C^{0,4}X_{Man} C^{1,3}X_{Man} C^{2,4}X_{Man} C^{0,4}X_{Man} C^{1,3}X_{Man} C^{2,4}X_{Man} ^{2,4}X_{NeuAc}Y ^{2,4}X_{NeuAc}Y
1652.53 0.5
1653.71 0.3
1669.76 1.2

Annotations:

^{0,4}X_{Man}Y ^{1,3}X_{Man}Y ^{2,4}X_{Man}Y Y^{0,4}X_{Man} Y^{1,3}X_{Man} Y^{2,4}X_{Man}
1670.68 23.8

Annotations:

B
1671.62 6.0
1687.66 1.1
1688.65 7.6

Annotations:

C
1689.65 2.4
1690.31 0.4
1738.04 0.5
1813.28 0.5

There are 2 similar spectra:

Id Structure Score

2726

0.80

2333

0.60
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