Efficient Chemoenzymatic Synthesis of an N-glycan Isomer Library

PubMed Entry

Peak Intensity
255.28 0.3

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BZ
256.11 3.7

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BY CZ BY CZ
257.2 0.5
258.14 0.2

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B^{1,5}X_{GlcNAc}
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273.3 0.5

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CY
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275.14 12.0
276.05 0.8

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ZZ
287.05 0.8

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^{1,3}A_{Man}Y ^{1,3}A_{Man}Y ^{2,4}A_{Man}Y C^{0,2}X_{Gal}
288.07 0.9

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C^{1,5}X_{Man}
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B
293.12 5.5

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B^{2,5}X_{GlcNAc}
294.1 0.3

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YY
295.04 1.4

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C^{2,5}X_{Gal} C^{0,2}X_{GlcNAc}
305.14 0.9
307.16 0.4

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BZ
309.15 0.2

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C^{1,4}X_{Man}
310.13 1.9

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C
314.24 1.9

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Z^{2,5}X_{Man} B^{3,5}X_{NeuAc} ^{1,5}A_{Man}Z
315.02 1.1

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B^{3,5}X_{NeuAc} ^{1,5}A_{Man}Z Y^{0,2}X_{Man}
318.19 0.8
325.16 3.1

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BY CZ
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BZ BY CZ
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343.14 0.9

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CY
347.19 0.5
348.15 1.2
350.22 9.4

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BY CZ
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^{2,4}A_{GlcNAc}Z C^{0,4}X_{NeuAc} B^{2,4}X_{NeuAc}
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C^{1,4}X_{NeuAc}
359.1 0.3

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^{1,3}A_{Man}Z ^{2,4}A_{Man}Z ^{1,3}A_{Man}Y C^{0,2}X_{Fuc}
364.17 0.7
364.88 2.2

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B^{0,3}X_{Fuc} ^{1,4}A_{Man}Z
365.45 3.1

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B^{0,3}X_{Fuc} ^{1,4}A_{Man}Z
366.17 655.3

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Z
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C^{2,5}X_{Fuc} ^{1,3}A_{Man}Y ^{2,4}A_{Man}Y
368.29 4.4

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CY
371.17 0.4

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C^{1,5}X_{Man} C^{2,4}X_{NeuAc}
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B^{1,3}X_{Fuc} B^{2,4}X_{Fuc}
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385.25 0.6
388.17 0.7

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^{1,5}A_{GlcNAc} ^{0,2}A_{Man}Z
405.27 0.8
407.26 7.2

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Z
419.23 6.9

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CY
420.11 4.4
420.98 1.6
421.62 0.2
425.25 43.4

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Y
426.2 6.8

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430.19 0.8
431.08 1.1

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^{1,3}A_{Man}Z ^{2,4}A_{Man}Z B^{0,2}X_{Fuc}
434.59 0.3

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C^{1,4}X_{NeuAc}
436.3 1.7
438.25 3.0

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B^{1,4}X_{Fuc}
439.12 1.2

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B^{2,5}X_{Fuc}
447.32 15.2

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Z
452.82 0.9

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B^{1,3}X_{Fuc} B^{2,4}X_{Fuc} ^{1,5}X_{Man}
454.23 41.2

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B
455.33 2.9

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C^{0,3}X_{Fuc} ^{1,4}A_{Man}Y
456.22 0.3

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Y
459.17 2.5

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^{1,5}A_{Man}Y
460.36 0.5
465.2 0.6
467.88 12.0
471.62 0.7
472.25 9.8

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C
473.09 1.3
475.94 1.6
476.87 17.8

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^{1,5}A_{Man}Z
487.29 0.4

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BY CZ
491.25 0.6
492.28 0.7

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C
493.18 0.4
495.25 5.6
501.22 0.6
503.02 0.3
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CY
509.26 2.7
510.36 0.6

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BZ
510.93 0.3

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BZ
511.56 1.4

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BZ
512.26 118.2

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BY CZ BY CZ
513.26 21.2

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^{1,3}A_{Man} ^{2,4}A_{Man}
514.32 1.6
520.37 8.7

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BY CZ
526.03 2.2
527.29 4.1

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^{2,4}X_{GlcNAc}
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BY CZ
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CY
530.29 1.7

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CY CY
532.35 0.4
533.24 2.0

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C^{1,5}X_{GlcNAc}
534.14 1.0

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B^{1,5}X_{Gal} ^{2,5}A_{Man}Z
537.23 1.5
540.63 0.8

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ZY YZ
544.06 0.6

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C^{0,2}X_{NeuAc} ^{0,3}A_{GlcNAc}
545.64 0.3
546.3 5.6

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CY
547.29 0.5
548.11 0.4
548.79 32.7

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YZ ZY
549.6 6.9

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YY
550.27 3.7

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C^{0,2}X_{Gal}
551.25 0.6

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ZZ
553.36 0.5
556.7 0.8

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B^{1,4}X_{Gal}
557.87 25.8

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YY
558.56 1.1

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C^{1,5}X_{Gal} C^{2,5}X_{Gal} ^{1,5}A_{Man}Y C^{0,3}X_{NeuAc}
559.27 0.7

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B^{1,4}X_{GlcNAc}
562.81 0.4

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BZ
564.15 1.6

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BY CZ
565.26 1.4

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C^{1,4}X_{Gal}
566.28 4.0

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C^{3,5}X_{NeuAc}
567.27 3.3
568.19 0.5
569.33 19.1

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YZ ZY
570.25 5.1

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B^{2,5}X_{Gal} B^{0,2}X_{GlcNAc} ^{0,2}X_{Gal}Y Y^{0,2}X_{Man}
571.34 4.9

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B^{1,3}X_{Gal} B^{2,4}X_{Gal} ^{1,5}X_{Fuc}Y Y^{1,5}X_{Gal}
573.37 0.6

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CY
576.22 0.5

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^{1,4}X_{Fuc}Z ^{1,4}X_{Gal}Z Z^{0,3}X_{Man} Z^{1,4}X_{Man}
577.8 0.3

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^{2,5}X_{Fuc}Z Z^{2,5}X_{Gal} B^{1,5}X_{Man}
584.5 0.7

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B^{1,5}X_{NeuAc} ^{1,4}A_{Man}Z ^{1,4}A_{Man}Z B^{1,4}X_{Gal} ^{0,3}X_{Fuc}Z Z^{3,5}X_{Man} Z^{1,4}X_{Gal}
585.34 0.4

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^{1,4}X_{Gal}Y B^{0,2}X_{Man} B^{0,4}X_{NeuAc} ^{1,4}X_{Fuc}Y Y^{0,3}X_{Man} Y^{1,4}X_{Man}
586.36 0.7

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B^{1,5}X_{Fuc} B^{2,5}X_{GlcNAc} ^{2,5}X_{Fuc}Y Y^{2,5}X_{Gal}
587.31 95.6

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YY
588.26 16.1

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C^{2,5}X_{Gal} C^{0,2}X_{GlcNAc}
589.02 1.0
592.78 10.3

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^{3,5}X_{Fuc}Z B^{0,2}X_{Fuc} B^{2,5}X_{Man}
593.4 2.2

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B^{0,2}X_{Fuc} B^{2,5}X_{Man} ^{0,3}X_{Fuc}Y Y^{1,4}X_{Gal} Y^{3,5}X_{Man}
595.24 1.2

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C^{1,5}X_{Fuc}
597.12 0.8

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C^{1,4}X_{NeuAc} ^{1,5}X_{Man}Z Z^{1,5}X_{Man}
598.19 0.4

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B^{2,5}X_{NeuAc}
600.29 6.1

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B^{1,4}X_{Man} C^{0,2}X_{NeuAc} B^{1,4}X_{Fuc} B^{0,3}X_{Man} B^{1,4}X_{Man} ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y ^{1,3}X_{Fuc}Y ^{2,4}X_{Fuc}Y Y^{0,4}X_{Man} Y^{1,3}X_{Man} Y^{2,4}X_{Man}
601.41 19.4

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CZ BY
606.16 0.8
608.28 0.2

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B^{0,3}X_{Fuc} B^{3,5}X_{Man} ^{0,4}X_{Fuc}Y Y^{1,3}X_{Gal} Y^{2,4}X_{Gal}
610.75 0.5

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Z^{0,2}X_{Man}
612.35 0.7

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ZZ
613.04 0.3

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ZZ
613.69 3.9
616.22 1.7

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C^{2,5}X_{NeuAc} B^{3,5}X_{Fuc} ^{2,5}A_{Man}
616.91 0.3

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C^{0,3}X_{Fuc} C^{3,5}X_{Man}
618.18 0.6

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C^{1,4}X_{Man}
622.26 2.6
623.33 0.2

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B^{0,4}X_{Fuc} B^{1,3}X_{NeuAc}
628.68 0.8

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B^{3,5}X_{NeuAc} ^{1,5}A_{Man}Z
629.99 17.7

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YZ ZY
630.82 6.5

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Y
631.35 3.3

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^{1,5}A_{Man}
634.14 0.6
635.47 2.6

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Z^{1,5}X_{Man}
636.37 0.7

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B^{0,2}X_{NeuAc}
637.04 1.5

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B^{1,5}X_{NeuAc}
638.99 30.5

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YY BZ
639.79 3.4
640.31 0.6
641.3 3.8

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^{1,4}X_{Man}Z Z^{0,3}X_{Man} Z^{1,4}X_{Man}
651.4 2.4

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B^{0,3}X_{NeuAc} ^{0,2}X_{Gal} Y^{0,2}X_{Man}
653.34 0.4

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C^{2,5}X_{NeuAc}
653.97 0.5

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C
654.63 0.8

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C
655.42 17.8
656.26 10.1

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BZ BZ
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BY CZ
658.31 230.0

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^{2,5}X_{Fuc}Z
659.24 17.2

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B^{3,5}X_{NeuAc} ^{1,4}X_{Man}Y Y^{0,3}X_{Man} Y^{1,4}X_{Man}
660.08 1.8

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^{0,2}X_{Fuc}Y Y^{2,5}X_{Man} ^{2,5}X_{Gal} C^{0,3}X_{NeuAc} ^{2,4}A_{GlcNAc}
662.29 0.2
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BZ
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BY CZ
666.85 1.0

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^{1,5}X_{NeuAc}Y ^{1,4}X_{Fuc}Y Y^{0,3}X_{Man} Y^{1,4}X_{Man} ^{1,4}X_{Gal}
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BZ
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BY CZ
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CZ BY CY
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CY
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682.07 0.4

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Y^{2,5}X_{NeuAc} ^{1,3}X_{Gal} ^{2,4}X_{Gal} ^{1,3}X_{Fuc}Y ^{2,4}X_{Fuc}Y Y^{0,4}X_{Man} Y^{1,3}X_{Man} Y^{2,4}X_{Man}
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CY
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688.11 0.6

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B^{1,5}X_{Fuc} Z^{3,5}X_{NeuAc} B^{1,4}X_{NeuAc}
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Y^{0,3}X_{NeuAc} ^{3,5}X_{NeuAc}Y C^{1,5}X_{Man} B^{1,4}X_{NeuAc} ^{1,3}X_{Man}Y Y^{0,4}X_{Man} Y^{1,3}X_{Man} Y^{2,4}X_{Man}
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BY CZ
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CY
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YZ ZY
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C^{1,5}X_{Fuc} Y^{3,5}X_{NeuAc}
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C^{1,4}X_{NeuAc}
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B^{0,2}X_{Fuc} ^{1,3}A_{Man}Z
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B^{1,4}X_{Fuc} B^{0,3}X_{Man} B^{1,4}X_{Man} B^{2,4}X_{NeuAc}
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Z
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C^{0,2}X_{Fuc} C^{2,5}X_{Man} ^{2,5}A_{GlcNAc}
704.82 0.2

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C^{2,4}X_{NeuAc}
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^{0,4}X_{NeuAc}Z
708.35 10.3

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CY
709.31 0.9

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B^{0,3}X_{Fuc} B^{3,5}X_{Man} ^{1,4}X_{NeuAc}Z
710.17 4.3

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B^{0,3}X_{Fuc} B^{3,5}X_{Man} ^{1,4}X_{NeuAc}Z
711.04 4.3

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C^{1,4}X_{Fuc} C^{0,3}X_{Man} C^{1,4}X_{Man}
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Y
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ZZ
714.54 0.8
715.35 3.8

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B^{2,5}X_{Fuc} ^{0,2}X_{Fuc}Z Z^{0,4}X_{NeuAc} Z^{0,2}X_{Man}
715.99 0.4

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^{1,3}A_{Man}Z ^{2,4}A_{Man}Z ^{0,4}X_{NeuAc}Y
725.13 1.2

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B^{0,4}X_{Fuc} B^{1,3}X_{NeuAc} Z^{2,4}X_{NeuAc}
726.94 0.7

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C^{3,5}X_{Fuc} Y^{1,4}X_{NeuAc}
729.08 1.8

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B^{0,3}X_{Fuc} ^{1,4}A_{Man}Z
730.34 0.5

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^{0,3}X_{Fuc}Z Z^{0,3}X_{Man} Z^{1,4}X_{Man}
731.35 142.1

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Z
732.31 28.1

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^{2,5}X_{Fuc}Y Y^{2,5}X_{Man}
733.44 22.6

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C^{2,5}X_{Fuc} C^{0,4}X_{Fuc} C^{1,3}X_{NeuAc} Y^{2,4}X_{NeuAc}
734.4 1.0

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^{1,3}A_{Man}Y ^{2,4}A_{Man}Y
735.01 1.8
736.56 1.4

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C^{1,5}X_{GlcNAc}
737.99 3.2

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^{3,5}X_{Fuc}Z Z^{3,5}X_{Man}
742.37 0.4
745.76 1.1

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^{0,4}X_{Fuc}Z ^{1,3}X_{NeuAc}Z Z^{0,4}X_{Man} Z^{1,3}X_{Man} Z^{2,4}X_{Man} B^{1,5}X_{NeuAc}
746.89 47.5

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B
747.44 7.9

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C^{0,3}X_{Fuc} ^{1,4}A_{Man}Y ^{3,5}X_{Fuc}Y Y^{3,5}X_{Man} ^{1,5}X_{NeuAc}
748.41 14.8
749.34 430.6

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Y
750.37 84.2
751.22 5.9
752.04 0.4
756.21 2.0

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C
758.86 0.3

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^{1,5}X_{GlcNAc}Z B^{0,4}X_{Fuc} B^{1,3}X_{NeuAc} Z^{1,5}X_{Man}
759.68 1.0

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^{1,5}X_{GlcNAc}Z B^{0,4}X_{Fuc} B^{1,3}X_{NeuAc} Z^{1,5}X_{Man}
762.23 1.3

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^{0,3}X_{NeuAc} B^{1,4}X_{GlcNAc} C^{0,2}X_{NeuAc} Y^{1,3}X_{NeuAc}
766.28 2.0
767.52 14.7

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Z
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770.35 1.0
771.17 0.3
774.42 1.4

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B^{0,3}X_{NeuAc}
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Z
776.43 55.4

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Y
777.25 5.6

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C^{0,4}X_{Fuc} C^{1,3}X_{NeuAc} ^{1,5}X_{Man} ^{1,5}X_{GlcNAc}Y Y^{1,5}X_{Man}
778.19 0.3

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C^{2,5}X_{NeuAc} ^{2,5}A_{Man}Y
780.77 0.4

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C^{1,4}X_{GlcNAc}
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783.38 2.6
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Y
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786.03 1.3
786.9 1.1
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B^{3,5}X_{NeuAc} ^{1,5}X_{Man} ^{1,5}A_{Man}Z
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^{0,2}A_{Man}Y
796.42 0.8
797.62 2.6

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^{1,4}X_{NeuAc}
800.38 0.9
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BZ
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BZ
802.47 13.0
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B
804.34 202.7
805.22 9.6

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^{0,2}X_{Fuc} ^{2,5}X_{Man}
807.36 2.8

Annotations:

C^{2,5}X_{GlcNAc} Y^{2,5}X_{Man}
811.22 0.7
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BZ
819.32 120.5

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BY CZ
820.34 27.3

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^{0,3}X_{Fuc} ^{3,5}X_{Man}
821.15 1.2

Annotations:

C
821.95 1.6
825.17 0.2
827.74 0.7

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^{1,3}X_{Fuc} ^{2,4}X_{Fuc} ^{0,4}X_{Man} ^{1,3}X_{Man} ^{2,4}X_{Man}
828.48 0.7

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^{3,5}X_{Fuc}
830.83 0.3
835.18 5.5

Annotations:

^{0,4}X_{Fuc} ^{1,3}X_{NeuAc}
836.37 283.5

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BY CZ
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Annotations:

CY
838.5 10.2
839.39 4.9
840.43 1.1
842.22 0.1
847.29 0.6

Annotations:

B^{1,5}X_{Fuc}
848.05 0.4

Annotations:

^{2,5}X_{GlcNAc}Y
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Annotations:

B^{2,4}X_{NeuAc} B^{1,5}X_{Gal}
870.49 0.7
871.48 1.7
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Annotations:

B^{1,4}X_{Fuc}
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877.42 49.4

Annotations:

B^{2,5}X_{Fuc}
878.37 18.2

Annotations:

B^{0,2}X_{Gal}
878.96 0.2

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^{1,3}A_{Man}Y ^{2,4}A_{Man}Y C^{0,2}X_{Fuc}
883.78 0.4
891.42 0.6

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B^{0,3}X_{Fuc} ^{1,4}A_{Man}Z
892.08 6.0

Annotations:

^{2,4}X_{GlcNAc}Y
892.64 2.7

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^{2,4}X_{GlcNAc}Y
893.37 337.4

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Z
894.41 103.0

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B^{2,5}X_{Gal}
895.4 199.7

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C^{2,5}X_{Fuc}
896.43 43.1

Annotations:

C^{0,2}X_{Gal}
897.38 2.8
898.67 0.4
907.51 0.3

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B^{3,5}X_{Fuc}
908.67 0.4

Annotations:

B^{1,4}X_{Gal}
909.22 2.3

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C^{0,3}X_{Fuc} ^{1,4}A_{Man}Y
910.41 27.9
911.37 1032.1

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Y
912.4 263.3

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C^{2,5}X_{Gal}
913.37 17.0
913.91 0.7
914.44 0.4
916.33 0.4
919.5 0.8
933.39 1.4
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Annotations:

^{1,5}A_{Man}
946.67 0.2
947.34 0.4
948.38 0.3
950.47 0.5
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952.4 4.8

Annotations:

^{1,5}A_{Man}Z
953.5 2.2
959.34 0.2
963.6 2.3
964.33 9.3
965.41 214.4

Annotations:

B
966.42 52.8
967.38 3.6
980.75 1.2

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BZ
981.4 12.9
982.54 2.6
983.49 0.8

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C
994.52 0.7

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^{0,2}X_{GlcNAc}
998.45 3.4

Annotations:

BY CZ
999.18 0.7
1005.34 0.8
1014.45 0.3
1017.22 0.2
1020.63 0.2
1022.59 2.4
1024.3 0.3
1037.33 1.1
1038.47 5.6
1039.45 282.8

Annotations:

BY CZ
1040.48 108.6
1041.47 4.8
1042.31 0.5
1054.59 1.1

Annotations:

^{2,4}X_{GlcNAc}
1055.5 74.7

Annotations:

^{1,4}A_{Man}
1056.58 42.5
1057.42 893.1

Annotations:

CY
1058.46 261.0
1059.48 25.2
1067.38 0.7

Annotations:

B^{1,5}X_{Gal} ^{2,5}A_{Man}Z
1073.47 8.0
1074.25 2.0
1074.96 0.2
1078.53 0.3

Annotations:

ZZ
1080.47 0.6

Annotations:

ZY YZ
1081.57 0.8

Annotations:

B^{0,2}X_{Gal}
1091.41 0.4
1094.71 0.4
1095.23 0.3
1096.48 19.4

Annotations:

YZ ZY
1097.51 7.7

Annotations:

B^{2,5}X_{Gal} ^{1,5}A_{Man}Z
1098.36 2.4

Annotations:

YY
1099.51 3.9

Annotations:

C^{0,2}X_{Gal}
1112.88 0.6
1113.4 4.4
1114.5 40.9

Annotations:

YY
1115.43 10.9

Annotations:

C^{2,5}X_{Gal} ^{1,5}A_{Man}Y
1116.78 1.1

Annotations:

C^{0,3}X_{NeuAc}
1127.5 6.5

Annotations:

BY CZ
1128.54 3.0
1129.04 0.5

Annotations:

C^{1,4}X_{Gal}
1129.7 0.6

Annotations:

C^{1,4}X_{Gal}
1131.56 1.4
1139.4 0.7
1141.37 0.5

Annotations:

B^{1,3}X_{Gal} B^{2,4}X_{Gal}
1141.94 0.7

Annotations:

^{1,5}X_{Fuc}Y Y^{1,5}X_{Gal}
1143.39 1.6

Annotations:

BY CZ
1144.45 0.9
1145.9 1.3

Annotations:

CY
1151.28 0.4
1157.65 1.4
1161.6 1.5

Annotations:

CY
1168.53 2.9

Annotations:

^{0,3}X_{Fuc}Z Z^{3,5}X_{Man} Z^{1,4}X_{Gal}
1182.66 0.5

Annotations:

^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z Z^{0,4}X_{Man} Z^{1,3}X_{Man} Z^{2,4}X_{Man}
1183.43 1.7

Annotations:

BZ
1184.51 12.6

Annotations:

^{3,5}X_{Fuc}Z
1185.46 4.9

Annotations:

B^{0,2}X_{Fuc} B^{2,5}X_{Man}
1186.49 0.7

Annotations:

^{0,3}X_{Fuc}Y Y^{1,4}X_{Gal} Y^{3,5}X_{Man}
1187.38 0.6

Annotations:

C^{0,2}X_{Man} C^{0,4}X_{NeuAc}
1201.54 54.2

Annotations:

BY CZ
1202.47 36.8

Annotations:

^{3,5}X_{Fuc}Y
1203.42 6.2

Annotations:

C^{0,2}X_{Fuc} C^{2,5}X_{Man}
1204.57 0.7
1209.34 1.1
1211.32 0.8
1218.35 0.6
1219.59 7.6

Annotations:

CY
1220.54 1.0
1228.11 0.5
1237.78 0.4
1242.54 12.5

Annotations:

Z
1243.61 4.1
1244.47 1.0
1258.54 38.4

Annotations:

ZY YZ
1259.56 13.0
1260.54 30.1

Annotations:

Y
1261.62 8.8

Annotations:

^{1,5}A_{Man}
1262.29 1.6
1274.17 1.6
1274.75 2.3
1275.55 3.6
1276.58 76.9

Annotations:

YY
1277.57 27.0
1278.55 4.0
1288.26 1.4

Annotations:

^{1,5}X_{Gal} Y^{1,5}X_{Man}
1289.66 2.3

Annotations:

B
1290.73 3.1
1307.63 1.0

Annotations:

C
1308.83 0.8
1309.54 0.6
1324.6 1.4
1328.6 0.7

Annotations:

BZ
1330.61 9.5

Annotations:

BY CZ
1331.52 4.1
1345.25 0.6
1346.56 27.1

Annotations:

BY CZ
1347.51 10.6
1348.67 18.6

Annotations:

CY
1349.53 9.3
1350.32 1.9
1353.19 0.7
1364.57 4.5

Annotations:

CY
1365.33 2.3
1382.24 0.4
1386.34 0.3
1404.63 33.4

Annotations:

Z
1405.63 12.9

Annotations:

YY
1406.53 1.8

Annotations:

C^{0,2}X_{Fuc} C^{2,5}X_{Man}
1420.69 1.4

Annotations:

C^{1,4}X_{Fuc} C^{0,3}X_{Man} C^{1,4}X_{Man}
1421.62 5.1
1422.61 135.8

Annotations:

Y
1423.66 48.4
1424.63 6.7
1425.79 0.8
1446.69 1.4
1470.65 0.6
1474.27 0.3
1475.52 0.3

Annotations:

^{3,5}X_{Fuc}Z Z^{3,5}X_{Man}
1492.59 55.2

Annotations:

B
1493.64 32.4

Annotations:

^{3,5}X_{Fuc}Y Y^{3,5}X_{Man}
1494.58 5.2

Annotations:

^{1,5}X_{NeuAc}
1509.64 0.2
1510.65 8.3

Annotations:

C
1511.67 5.9
1512.59 0.6
1567.77 1.5

Annotations:

Y
1568.6 0.5

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