Efficient Chemoenzymatic Synthesis of an N-glycan Isomer Library

PubMed Entry

Peak Intensity
366.29 3.9

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YZ ZY YZ ZY
418.11 1.2
436.2 1.6
453.62 1.8

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B^{1,3}X_{Fuc} B^{2,4}X_{Fuc} B^{1,3}X_{Fuc} B^{2,4}X_{Fuc} ^{1,5}X_{Man}
454.29 1.1

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B B
512.27 8.5

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BY CZ CZ BY CZ BY CZ BY
513.31 2.6

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^{1,3}A_{Man} ^{0,4}A_{Man} ^{2,4}A_{Man}
528.21 3.8

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^{3,5}A_{GlcNAc} ^{0,3}A_{Man} ^{1,4}A_{Man}
529.25 2.1
570.36 2.2

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B^{2,5}X_{Gal} B^{2,5}X_{Gal} ^{0,2}X_{Gal}Y Y^{0,2}X_{Gal}
640.18 0.8
656.35 1.3

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BZ BZ BZ BZ
657.39 20.9

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BY BY CZ CZ
658.36 13.5
674.33 2.3

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CZ BY CY CY CZ BY
690.31 0.5

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C^{1,4}X_{GlcNAc}
731.36 3.8

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YZ ZY YZ ZY
749.13 0.7

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YY YY
802.51 0.5
803.39 18.2

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B B
804.39 15.2

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^{2,5}X_{Fuc}Z Z^{2,5}X_{Fuc}
813.4 3.0

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^{2,5}X_{Fuc}Y Y^{2,5}X_{Fuc}
819.45 3.0

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BY BY CZ CZ BY BY CZ CZ
822.36 0.4
836.35 1.0
856.2 2.0

Annotations:

B^{1,4}X_{Gal} B^{1,4}X_{Gal} ^{2,4}X_{GlcNAc}Y ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y Y^{1,3}X_{Gal} Y^{2,4}X_{Gal}
857.89 1.7

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C^{2,5}X_{Gal} C^{2,5}X_{Gal}
893.48 5.4

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YZ ZY
894.48 1.0
911.53 3.0

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YY
949.5 15.5

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ZZ YZ ZY ZY ZY YZ ZY YZ YZ
950.52 7.8

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^{1,5}A_{Man}Z ^{1,5}A_{Man}Z B^{2,5}X_{Gal} B^{2,5}X_{Gal} B^{0,3}X_{NeuAc} B^{0,3}X_{NeuAc}
965.53 4.5

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B B
966.53 1.5

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C^{1,4}X_{Gal} C^{1,4}X_{Gal}
967.35 1.7

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YY
1002.81 3.4

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BY BY CZ CZ
1010.96 1.2

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CY CY
1020.2 1.1
1023.73 1.2
1031.52 3.5

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Y Y
1039.45 3.7

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YY YY YY YY
1055.45 2.4

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C
1056.64 0.8
1057.45 2.9
1070.86 2.7
1093.73 0.8
1094.47 2.6

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ZZ
1095.66 2.8

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B^{0,2}X_{Fuc} B^{0,2}X_{Fuc}
1096.62 0.5

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C^{0,4}X_{NeuAc} C^{0,4}X_{NeuAc}
1111.38 1.9

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B^{0,3}X_{Fuc} B^{0,3}X_{Fuc}
1112.43 1.2

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Y Y
1112.98 1.8

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Y Y
1113.73 8.5
1114.55 4.7
1115.48 1.2
1136.37 0.9
1168.54 1.2
1176.9 2.8

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Z Z
1185.82 3.9

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Y Y
1201.51 1.5
1202.62 2.7
1203.58 1.2
1205.52 1.2
1219.71 1.0
1220.6 1.6
1225.68 1.6
1242.4 1.7

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ZY YZ YZ YZ YZ ZY ZY ZY
1258.56 0.9
1259.6 2.4
1260.45 1.3

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YY YY YY YY
1261.64 1.6

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^{1,5}A_{Man}Y ^{1,5}A_{Man}Y
1275.22 2.3
1276.52 5.4
1278.27 0.8
1289.46 1.3

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BY BY CZ CZ
1328.33 1.2

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^{2,5}X_{NeuAc}Z Z^{2,5}X_{NeuAc}
1330.69 1.6

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BY BY CZ CZ
1346.67 3.5

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^{2,5}X_{NeuAc}Y Y^{2,5}X_{NeuAc}
1347.83 1.0
1348.61 0.9

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CY CY
1388.6 2.6

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B^{2,5}X_{GlcNAc}
1403.7 1.6

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^{2,4}X_{GlcNAc}Y
1404.56 1.2

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YZ YZ ZY ZY
1405.17 1.2

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YY YY
1405.71 5.1

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YY YY
1406.63 4.1

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C^{2,5}X_{GlcNAc}
1407.65 1.4
1421.64 0.8
1422.67 5.1

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YY YY
1423.71 2.9
1424.49 0.6
1429.41 1.1

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^{0,2}X_{Fuc}Z Z^{0,2}X_{Fuc}
1440.01 1.1
1445.72 1.3

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^{2,5}X_{Fuc}Z Z^{2,5}X_{Fuc}
1463.76 0.6

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^{2,5}X_{Fuc}Y Y^{2,5}X_{Fuc}
1475.81 1.7

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^{3,5}X_{Fuc}Z Z^{3,5}X_{Fuc}
1492.67 3.9

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BY BY CZ CZ
1493.72 1.2

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^{3,5}X_{Fuc}Y Y^{3,5}X_{Fuc}
1533.67 1.7

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Z Z
1534.65 2.6
1550.1 4.0
1550.81 4.9
1551.67 9.8

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Y Y
1552.62 6.1
1553.16 1.2
1566.58 3.5
1567.66 25.7

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YY YY
1568.73 38.7
1569.74 2.3
1605.29 1.0

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^{1,4}X_{Fuc}Z Z^{1,4}X_{Fuc}
1622.7 4.6
1623.72 2.1

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^{1,4}X_{Fuc}Y Y^{1,4}X_{Fuc} ^{1,4}X_{Man} ^{1,4}X_{Man}
1626.6 1.6
1638.87 3.9

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BY BY CZ CZ
1657.65 0.9
1679.83 0.7
1695.95 5.1

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Z Z
1696.9 7.2

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B^{2,5}X_{Gal} B^{2,5}X_{Gal}
1697.81 2.3
1698.76 8.8

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C^{0,2}X_{Gal} C^{0,2}X_{Gal}
1711.18 1.5

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^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y Y^{1,3}X_{Gal} Y^{2,4}X_{Gal}
1712.49 1.4
1713.71 36.9

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Y Y
1714.69 33.4

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C^{2,5}X_{Gal} C^{2,5}X_{Gal}
1715.73 1.8
1754.85 1.1

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^{1,5}A_{Man}Z ^{1,5}A_{Man}Z
1768.03 2.1
1771.66 2.1

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YY YY
1787.83 1.3
1788.89 1.1
1802.92 1.7
1840.27 1.0
1842.01 6.6

Annotations:

BY BY CZ CZ
1842.89 10.4
1858.08 2.9

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^{2,5}X_{NeuAc}Y Y^{2,5}X_{NeuAc}
1859.05 1.5
1859.73 22.0

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CY CY
1860.8 53.2
1861.43 8.9
1861.99 2.7
1933.62 2.0

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YY
1986.82 1.2

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^{3,5}X_{Fuc}Z Z^{3,5}X_{Fuc} Z^{3,5}X_{Fuc} Z^{3,5}X_{Fuc}
1987.86 1.4

Annotations:

B^{0,2}X_{Fuc} ^{1,5}X_{NeuAc}Z Z^{1,5}X_{NeuAc} B^{0,2}X_{Fuc}

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