Mucin-type proteins produced in the Trichoplusia ni and Spodoptera frugiperda insect cell lines carry O-glycans with phosphocholine and sulfate substitutions
| Peak | Intensity |
|---|---|
| 240.96 | 1.5 |
| 241.72 | 0.1 |
| 243.25 | 0.2 |
| 252.03 | 0.1 |
| 255.1 | 0.2 |
| 257.12 | 0.1 |
| 262.15 | 0.3 |
|
Annotations: ^{2,4}A_{HexA} |
|
| 267.89 | 0.3 |
| 276.98 | 0.2 |
|
Annotations: B^{2,4}X_{HexA} |
|
| 282.06 | 26.4 |
|
Annotations: B |
|
| 283.02 | 2.1 |
| 283.81 | 0.2 |
| 284.26 | 0.3 |
| 288.99 | 0.1 |
| 290.11 | 0.2 |
|
Annotations: ^{3,5}A_{HexA} |
|
| 298.03 | 0.4 |
| 298.88 | 0.1 |
| 300.05 | 1.3 |
|
Annotations: C |
|
| 300.97 | 0.1 |
| 302.1 | 3.3 |
| 303.13 | 0.3 |
| 306.14 | 0.1 |
|
Annotations: ^{0,3}A_{HexA} |
|
| 307.2 | 0.1 |
| 310.11 | 0.1 |
| 311.17 | 0.1 |
| 314.02 | 0.1 |
| 317.97 | 0.1 |
| 318.17 | 0.1 |
| 321.22 | 0.1 |
| 322.08 | 0.1 |
| 325.67 | 0.1 |
| 326.07 | 0.1 |
| 326.56 | 0.1 |
| 332.18 | 0.7 |
| 332.4 | 0.2 |
| 333.11 | 0.1 |
| 333.4 | 0.1 |
| 334.16 | 0.1 |
| 337.13 | 0.1 |
|
Annotations: BY |
|
| 338.17 | 0.1 |
| 338.94 | 0.1 |
| 340.03 | 0.1 |
| 340.31 | 0.1 |
| 342.2 | 0.1 |
|
Annotations: ^{2,4}A_{HexA} |
|
| 343.13 | 0.1 |
| 344.14 | 0.1 |
| 346.16 | 0.1 |
| 352.1 | 0.1 |
| 356.14 | 0.1 |
| 359.01 | 0.1 |
| 367.09 | 0.1 |
| 368.13 | 0.1 |
| 368.46 | 0.1 |
| 368.78 | 0.1 |
| 369.91 | 0.1 |
|
Annotations: ^{3,5}A_{HexA} |
|
| 371.04 | 0.1 |
| 376.13 | 0.1 |
| 376.48 | 0.1 |
| 376.95 | 0.2 |
| 377.99 | 0.6 |
|
Annotations: B |
|
| 378.27 | 0.5 |
|
Annotations: B |
|
| 379.94 | 0.1 |
| 382.21 | 0.2 |
| 384.23 | 0.9 |
|
Annotations: Y |
|
| 384.88 | 0.1 |
| 385.25 | 0.3 |
| 386.11 | 0.1 |
|
Annotations: ^{0,3}A_{HexA} |
|
| 387.43 | 0.1 |
| 389.91 | 0.1 |
| 391.15 | 0.1 |
| 392.74 | 0.2 |
| 394.3 | 0.3 |
| 394.99 | 0.1 |
| 395.9 | 0.4 |
|
Annotations: C |
|
| 396.27 | 0.2 |
|
Annotations: C |
|
| 397.14 | 0.2 |
| 398.19 | 0.2 |
| 399.49 | 0.1 |
| 401.93 | 0.1 |
| 403.18 | 0.1 |
| 404.42 | 0.2 |
| 405.44 | 0.2 |
| 408.23 | 0.2 |
| 409.81 | 0.1 |
| 410.14 | 0.1 |
| 411.87 | 0.1 |
|
Annotations: ^{1,5}X_{HexA} |
|
| 412.76 | 0.1 |
| 414.22 | 0.1 |
| 414.97 | 0.1 |
| 416.17 | 0.2 |
|
Annotations: ^{0,2}A_{HexA} |
|
| 422.24 | 0.1 |
| 425.5 | 0.1 |
| 425.91 | 0.1 |
|
Annotations: ^{0,2}X_{HexA} |
|
| 426.21 | 0.1 |
|
Annotations: ^{0,2}X_{HexA} |
|
| 426.97 | 0.1 |
| 429.1 | 0.1 |
| 431.92 | 0.1 |
| 432.5 | 0.1 |
| 433.31 | 0.2 |
| 436.2 | 0.2 |
| 436.58 | 0.2 |
| 440.28 | 0.2 |
| 441.0 | 0.1 |
| 442.28 | 0.1 |
|
Annotations: ^{2,5}X_{HexA} |
|
| 444.19 | 0.4 |
| 446.06 | 0.3 |
| 446.36 | 0.1 |
| 447.48 | 0.1 |
| 447.95 | 0.2 |
| 448.22 | 0.3 |
| 448.67 | 0.1 |
| 449.91 | 0.1 |
| 451.05 | 0.1 |
| 454.25 | 0.9 |
| 456.12 | 0.1 |
|
Annotations: ^{0,3}X_{HexA} |
|
| 458.11 | 51.4 |
|
Annotations: B |
|
| 459.15 | 5.7 |
| 461.29 | 0.2 |
| 462.12 | 0.5 |
| 463.01 | 0.6 |
| 463.23 | 0.2 |
| 464.17 | 161.6 |
| 465.15 | 23.9 |
| 468.18 | 0.2 |
|
Annotations: ^{0,4}X_{HexA}Z |
|
| 474.03 | 0.2 |
| 474.35 | 0.5 |
| 475.48 | 0.2 |
| 476.02 | 0.5 |
|
Annotations: C |
|
| 477.06 | 0.1 |
| 478.17 | 0.5 |
| 478.41 | 0.2 |
| 478.97 | 0.1 |
| 479.4 | 0.1 |
| 480.25 | 0.3 |
|
Annotations: B^{1,3}X_{HexA} |
|
| 482.11 | 0.1 |
|
Annotations: ^{1,3}X_{HexA}Z |
|
| 482.91 | 0.1 |
| 485.71 | 0.2 |
|
Annotations: ^{0,4}X_{HexA}Y |
|
| 487.06 | 0.1 |
| 491.19 | 0.2 |
| 493.18 | 0.1 |
| 495.02 | 0.1 |
| 495.94 | 0.3 |
| 497.02 | 0.1 |
| 498.25 | 17.1 |
|
Annotations: C^{1,3}X_{HexA} |
|
| 499.21 | 3.6 |
| 501.95 | 0.1 |
| 506.25 | 0.9 |
| 508.91 | 0.1 |
| 511.32 | 0.1 |
| 515.95 | 0.1 |
| 517.39 | 0.1 |
| 518.4 | 0.1 |
| 519.99 | 0.1 |
| 520.81 | 0.1 |
| 521.13 | 0.1 |
| 522.09 | 0.4 |
| 522.52 | 0.1 |
| 524.23 | 4.7 |
| 524.95 | 0.3 |
| 525.34 | 0.5 |
| 526.5 | 0.1 |
| 527.68 | 0.1 |
| 528.25 | 0.1 |
| 530.9 | 0.1 |
| 531.94 | 0.1 |
| 532.85 | 0.1 |
| 538.15 | 0.1 |
| 538.97 | 0.1 |
| 540.23 | 1.2 |
|
Annotations: B |
|
| 542.23 | 114.9 |
|
Annotations: Z |
|
| 543.22 | 23.0 |
| 544.3 | 1.4 |
| 545.13 | 0.2 |
| 545.52 | 0.1 |
| 546.21 | 0.2 |
|
Annotations: B^{0,4}X_{HexA} |
|
| 547.39 | 0.5 |
| 549.18 | 0.1 |
| 550.1 | 0.3 |
| 551.25 | 0.1 |
| 552.27 | 0.1 |
| 552.82 | 0.3 |
| 553.64 | 0.1 |
| 554.69 | 0.1 |
| 555.02 | 0.2 |
| 555.79 | 0.1 |
| 556.8 | 0.5 |
| 558.4 | 0.5 |
|
Annotations: C |
|
| 559.25 | 1.7 |
| 560.25 | 5.2 |
|
Annotations: Y |
|
| 561.28 | 1.5 |
| 562.35 | 0.2 |
| 563.06 | 0.4 |
| 563.84 | 0.1 |
|
Annotations: C^{0,4}X_{HexA} |
|
| 569.02 | 0.1 |
| 570.65 | 0.1 |
| 573.31 | 0.1 |
| 574.19 | 0.1 |
| 575.09 | 0.2 |
| 577.01 | 0.1 |
| 577.98 | 0.1 |
|
Annotations: C^{1,3}X_{HexA} |
|
| 578.2 | 0.2 |
|
Annotations: C^{1,3}X_{HexA} |
|
| 578.4 | 0.1 |
|
Annotations: C^{1,3}X_{HexA} |
|
| 581.1 | 0.3 |
| 581.49 | 0.1 |
| 582.18 | 0.1 |
| 582.5 | 0.2 |
| 583.25 | 0.4 |
| 583.95 | 0.1 |
| 584.33 | 0.1 |
| 585.69 | 0.1 |
| 585.97 | 0.1 |
| 587.03 | 0.1 |
| 589.07 | 0.1 |
| 590.11 | 0.3 |
| 591.04 | 0.2 |
| 592.0 | 0.1 |
| 593.07 | 0.3 |
| 594.17 | 0.2 |
| 595.19 | 0.2 |
| 596.27 | 0.1 |
| 598.3 | 0.3 |
| 599.06 | 0.1 |
| 600.23 | 0.1 |
| 600.58 | 0.2 |
| 601.02 | 1.5 |
| 601.32 | 1.6 |
| 601.71 | 0.4 |
| 602.05 | 0.2 |
| 602.3 | 0.3 |
| 602.98 | 0.1 |
| 603.32 | 0.1 |
| 604.15 | 0.1 |
| 606.88 | 0.2 |
| 607.1 | 0.2 |
| 608.64 | 0.1 |
| 609.04 | 0.2 |
| 613.19 | 0.1 |
| 613.69 | 0.1 |
| 615.98 | 0.1 |
| 616.92 | 0.1 |
| 617.24 | 0.1 |
| 618.8 | 0.3 |
| 619.37 | 1.0 |
| 620.4 | 0.7 |
|
Annotations: B |
|
| 620.72 | 0.4 |
| 621.17 | 1.6 |
| 621.44 | 1.0 |
| 621.65 | 0.4 |
| 622.49 | 6.5 |
| 623.34 | 0.3 |
| 624.28 | 0.2 |
| 625.25 | 0.2 |
| 626.07 | 0.5 |
| 627.41 | 0.1 |
| 627.69 | 0.1 |
| 628.94 | 0.1 |
| 630.19 | 0.1 |
| 631.61 | 0.1 |
| 632.31 | 0.1 |
| 632.9 | 0.2 |
| 633.48 | 0.1 |
| 633.89 | 0.1 |
| 634.42 | 0.2 |
| 635.23 | 0.4 |
| 635.92 | 0.2 |
| 636.81 | 0.1 |
| 638.13 | 0.1 |
|
Annotations: C |
|
| 638.76 | 0.3 |
| 639.31 | 0.3 |
| 640.3 | 4.8 |
| 641.11 | 1.5 |
| 641.84 | 0.1 |
| 642.65 | 0.2 |
| 643.29 | 0.1 |
| 643.94 | 0.1 |
| 645.12 | 0.1 |
| 645.82 | 0.1 |
| 646.31 | 0.3 |
| 646.95 | 0.1 |
| 647.34 | 0.1 |
| 648.09 | 0.2 |
| 648.93 | 0.2 |
| 649.41 | 0.1 |
| 650.71 | 0.1 |
| 651.01 | 0.1 |
| 651.75 | 0.1 |
| 652.16 | 0.1 |
| 653.1 | 0.1 |
| 654.75 | 0.1 |
| 655.08 | 0.1 |
| 656.23 | 0.2 |
| 656.78 | 0.3 |
| 657.86 | 0.1 |
| 658.14 | 0.1 |
| 659.35 | 0.1 |
| 659.76 | 0.3 |
| 660.42 | 0.1 |
| 661.09 | 0.1 |
| 661.58 | 0.3 |
| 662.07 | 0.1 |
| 663.28 | 0.1 |
| 664.03 | 0.5 |
| 664.25 | 0.1 |
| 665.16 | 0.2 |
| 665.79 | 0.1 |
| 667.13 | 0.1 |
| 667.82 | 0.2 |
| 668.93 | 0.1 |
| 670.01 | 0.1 |
| 670.52 | 0.1 |
| 672.15 | 0.3 |
| 673.16 | 0.4 |
| 673.66 | 0.1 |
| 675.04 | 0.1 |
| 676.09 | 0.2 |
| 678.2 | 0.3 |
| 679.13 | 0.1 |
| 679.71 | 0.1 |
| 680.95 | 0.3 |
| 681.25 | 0.5 |
| 681.96 | 0.3 |
| 683.23 | 0.1 |
| 683.91 | 0.5 |
| 685.14 | 0.2 |
| 686.68 | 0.1 |
| 687.03 | 0.1 |
| 688.7 | 0.1 |
| 690.16 | 0.1 |
| 690.89 | 0.1 |
| 691.91 | 0.1 |
| 692.77 | 0.1 |
| 693.23 | 0.3 |
| 693.81 | 0.1 |
| 695.34 | 0.2 |
| 696.58 | 0.1 |
| 697.37 | 0.1 |
| 698.26 | 0.1 |
| 698.77 | 0.1 |
| 699.45 | 0.1 |
| 699.76 | 0.1 |
| 700.08 | 0.2 |
| 700.48 | 0.2 |
| 700.79 | 0.1 |
| 701.42 | 0.1 |
| 702.07 | 0.1 |
| 703.34 | 0.2 |
|
Annotations: ^{1,3}X_{HexA} |
|
| 705.13 | 0.1 |
| 707.03 | 0.1 |
| 707.75 | 0.1 |
| 708.53 | 0.2 |
| 709.11 | 0.4 |
| 709.89 | 0.1 |
| 710.29 | 0.2 |
| 711.07 | 0.6 |
| 711.6 | 0.2 |
| 712.09 | 0.2 |
| 712.39 | 0.3 |
| 712.87 | 0.4 |
| 713.73 | 0.1 |
| 714.09 | 0.1 |
| 714.39 | 0.1 |
| 714.79 | 0.2 |
| 716.21 | 0.1 |
| 717.25 | 0.1 |
| 718.12 | 0.2 |
| 719.32 | 1.0 |
| 719.66 | 0.1 |
| 720.52 | 0.1 |
| 721.56 | 0.1 |
| 723.12 | 0.2 |
| 723.31 | 0.1 |
| 725.05 | 0.2 |
| 725.49 | 0.1 |
| 727.12 | 0.1 |
| 727.47 | 0.1 |
| 727.97 | 0.1 |
| 728.24 | 0.1 |
| 729.02 | 0.2 |
| 729.31 | 0.3 |
| 730.16 | 0.5 |
| 735.48 | 0.1 |
| 736.09 | 0.1 |
| 736.87 | 0.1 |
| 737.24 | 0.1 |
| 738.04 | 0.5 |
| 739.06 | 0.3 |
| 739.48 | 0.1 |
| 739.87 | 0.1 |
| 740.7 | 0.1 |
| 741.09 | 0.2 |
| 741.84 | 0.3 |
| 743.3 | 1.7 |
| 744.34 | 7.3 |
| 745.27 | 10.1 |
| 746.34 | 1.2 |
| 747.37 | 1.1 |
| 747.94 | 0.1 |
| 748.38 | 0.1 |
| 749.02 | 0.1 |
| 749.33 | 0.2 |
| 751.39 | 0.1 |
| 751.87 | 0.1 |
| 752.24 | 0.2 |
| 753.12 | 1.3 |
| 754.41 | 0.3 |
| 755.24 | 0.6 |
| 755.79 | 0.1 |
| 757.27 | 0.1 |
| 758.41 | 0.4 |
| 759.4 | 0.4 |
| 759.87 | 0.3 |
| 760.18 | 0.1 |
| 761.25 | 9.9 |
| 762.26 | 60.3 |
| 763.3 | 6383.3 |
| 764.28 | 1475.1 |
| 765.07 | 0.9 |
| 765.97 | 0.1 |
| 766.24 | 0.3 |
| 766.79 | 0.2 |
| 767.01 | 0.1 |
| 767.48 | 0.1 |
| 767.89 | 0.3 |
| 768.21 | 0.1 |
| 768.6 | 0.2 |
| 769.17 | 1.0 |
|
Annotations: ^{0,4}X_{HexA} |
|
| 770.25 | 0.1 |
| 771.26 | 0.3 |
| 771.89 | 0.3 |
| 773.98 | 0.1 |
| 774.73 | 0.1 |
| 775.47 | 0.1 |
| 776.09 | 0.1 |
| 776.37 | 0.2 |
| 778.17 | 0.1 |
| 779.41 | 0.1 |
| 779.8 | 0.5 |
| 780.28 | 0.1 |
| 780.51 | 0.1 |
| 781.03 | 0.6 |
| 781.66 | 0.2 |
| 782.07 | 0.6 |
| 782.52 | 0.2 |
| 783.19 | 1.2 |
|
Annotations: ^{1,3}X_{HexA} |
|
| 784.35 | 0.5 |
| 784.73 | 0.3 |
| 785.09 | 0.1 |
| 785.8 | 0.2 |
| 787.02 | 0.1 |
| 787.87 | 0.2 |
| 788.71 | 0.1 |
| 789.2 | 0.2 |
| 790.16 | 0.1 |
| 794.09 | 0.1 |
| 794.81 | 0.1 |
| 796.07 | 0.8 |
| 796.68 | 0.2 |
| 797.02 | 0.4 |
| 797.35 | 0.5 |
| 797.62 | 0.3 |
| 797.95 | 1.3 |
| 798.38 | 0.6 |
| 798.78 | 0.5 |
| 799.09 | 0.7 |
| 799.53 | 0.5 |
| 799.8 | 0.3 |
| 800.18 | 0.2 |
| 801.24 | 0.2 |
| 806.02 | 0.6 |
| 806.54 | 0.1 |
| 807.29 | 0.3 |
| 807.61 | 0.3 |
| 807.97 | 0.2 |
| 808.47 | 0.2 |
| 809.07 | 0.5 |
| 809.36 | 0.3 |
| 809.59 | 0.2 |
| 810.05 | 0.1 |
| 810.78 | 0.1 |
| 812.26 | 0.2 |
| 814.27 | 0.2 |
| 816.62 | 0.1 |
| 823.45 | 0.1 |
There are not related spectra