Mucin-type proteins produced in the Trichoplusia ni and Spodoptera frugiperda insect cell lines carry O-glycans with phosphocholine and sulfate substitutions

PubMed Entry

GlyToucan Accession Number: G83189OV

Peak Intensity
213.96 0.5
214.33 0.4
216.25 0.2
216.98 0.5

Annotations:

^{2,4}A_{Gal}Z
217.15 0.4

Annotations:

^{2,4}A_{Gal}Z
220.06 1.0

Annotations:

C
220.96 1.1

Annotations:

C^{1,5}X_{HexNAc} C^{0,2}X_{HexA}
221.19 0.7

Annotations:

C^{1,5}X_{HexNAc} C^{0,2}X_{HexA}
222.11 259.0

Annotations:

Y
223.12 21.8
226.14 0.8
227.23 2.6
227.87 0.2
228.12 0.6
228.99 0.4
230.97 1.9

Annotations:

^{3,5}A_{Gal}Z
231.77 0.1
232.26 1.2
233.03 0.1

Annotations:

B^{0,3}X_{HexA}
233.99 0.7
235.15 0.3

Annotations:

^{2,4}A_{Gal}Y
237.68 0.4
238.91 0.1
240.51 0.3
241.12 0.1
241.91 0.1
242.26 0.1
243.06 1.5
244.03 1.3
246.08 10.5
247.02 0.3

Annotations:

B^{1,4}X_{HexA} ^{0,3}A_{Gal}Z ^{1,4}A_{Gal}Z B^{1,4}X_{HexNAc}
249.09 1.0

Annotations:

B^{3,5}X_{HexA} ^{3,5}A_{Gal}Y
253.3 0.3
254.68 0.3
255.18 0.5
256.09 0.5
257.09 1.1
257.4 0.4
257.78 1.0

Annotations:

B^{0,2}X_{HexNAc}
258.12 2.4

Annotations:

B^{0,2}X_{HexNAc}
258.32 1.5

Annotations:

B^{0,2}X_{HexNAc}
259.14 0.5
260.85 0.2

Annotations:

^{2,5}A_{Gal}Z
261.4 0.6

Annotations:

^{2,5}A_{Gal}Z
261.85 1.0

Annotations:

^{2,4}A_{HexA}
262.35 0.8

Annotations:

^{2,4}A_{HexA}
263.35 1.3
263.74 0.5

Annotations:

^{0,2}X_{Gal}
264.26 0.8

Annotations:

^{0,2}X_{Gal}
265.15 0.3

Annotations:

C^{1,4}X_{HexA} ^{0,3}A_{Gal}Y ^{1,4}A_{Gal}Y C^{1,4}X_{HexNAc}
265.71 0.1
266.28 0.6
270.31 0.2
271.2 0.2
271.95 0.1
274.18 2.8

Annotations:

B^{2,5}X_{HexNAc}
275.06 3.6
275.45 0.6
276.13 8.5

Annotations:

C^{0,2}X_{HexNAc}
276.99 0.7

Annotations:

B^{2,4}X_{HexA} B^{1,3}X_{HexNAc} ^{0,2}A_{Gal}Z
277.15 0.5

Annotations:

B^{2,4}X_{HexA} B^{1,3}X_{HexNAc} ^{0,2}A_{Gal}Z
278.23 0.2
278.4 0.1
281.04 0.9
282.12 0.2
282.78 1.0
286.12 2.2
287.2 2.0
288.17 2.2

Annotations:

B^{0,3}X_{HexNAc}
289.41 0.4
292.07 7.1

Annotations:

C^{2,5}X_{HexNAc} ^{1,4}A_{HexA}
293.15 2.8
293.79 0.3

Annotations:

^{0,3}X_{Gal} ^{1,4}X_{Gal}
294.26 0.1

Annotations:

^{0,3}X_{Gal} ^{1,4}X_{Gal}
296.34 0.2
299.15 0.3
300.11 0.4
300.98 0.7
304.07 1.5

Annotations:

B^{3,5}X_{HexNAc}
305.23 0.1
306.23 3.5

Annotations:

^{0,3}A_{HexA} C^{0,3}X_{HexNAc} ^{0,4}X_{Gal}Z ^{1,3}X_{Gal}Z
307.1 0.2
309.08 0.3

Annotations:

^{1,5}A_{Gal}Y
313.59 0.3
316.03 1.3
318.1 14.8

Annotations:

B^{0,4}X_{HexNAc} B^{2,4}X_{HexNAc}
319.11 15.2

Annotations:

BZ
320.04 3.6

Annotations:

^{2,5}A_{HexA}
320.44 0.9

Annotations:

^{2,5}A_{HexA}
324.18 7.8

Annotations:

^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}
325.36 0.9
328.02 2.5
328.88 0.2
329.16 0.2
330.14 3.8
331.38 0.5
332.14 7.3
333.22 7.3
334.1 10.8
335.12 1.4
336.07 0.4

Annotations:

^{0,2}A_{HexA} C^{0,4}X_{HexNAc} C^{2,4}X_{HexNAc}
337.09 10.3

Annotations:

BY CZ
338.18 1.9
338.4 0.7
339.1 0.9
340.26 0.1
342.33 0.1
343.16 1.2
345.62 0.5
346.08 3.3
346.34 1.5
347.04 0.8
347.3 1.7
348.2 5.4
348.9 0.3
349.21 0.2
349.99 0.2

Annotations:

^{1,5}A_{HexA}
352.09 1.0
352.38 0.2
353.8 0.2
358.09 0.4
360.23 0.9
361.0 0.2
362.28 0.1
364.04 0.4
364.3 0.5
364.97 0.3

Annotations:

B^{1,5}X_{HexNAc}
365.4 2.1

Annotations:

B^{1,5}X_{HexNAc}
366.16 79.9

Annotations:

Z
367.29 4.4
368.24 0.9
371.94 2.2
372.21 1.9
372.95 0.4
375.47 0.2
375.99 1.0
376.92 2.2
377.19 1.4
377.56 0.7
378.17 2.9

Annotations:

B
379.36 0.2
380.19 1.8
381.13 0.2
382.3 0.2
383.39 0.3

Annotations:

C^{1,5}X_{HexNAc}
384.18 144.9

Annotations:

Y
385.2 15.0
386.11 2.8
386.61 0.3
388.3 0.3
390.18 58.2
391.11 11.5
391.98 0.3
392.33 0.3
394.2 7.8
395.19 0.4
396.14 20.3

Annotations:

C
397.09 22.5
398.15 4.5
400.42 0.5
402.09 0.1
406.37 0.8
407.94 1.6
408.26 1.3
409.44 0.2

Annotations:

B^{1,4}X_{HexNAc}
409.88 0.1
410.13 0.1
412.12 0.5

Annotations:

^{1,5}X_{HexA}
413.89 0.5
414.12 0.7
414.98 0.2
416.38 1.1
418.18 0.4
418.47 0.1
420.19 2.7

Annotations:

B^{0,2}X_{HexNAc}
420.96 0.8
421.3 1.4
422.16 0.1
422.88 0.2
424.25 0.9
426.34 8.4

Annotations:

^{0,2}X_{HexA}
427.58 0.4

Annotations:

C^{1,4}X_{HexNAc}
430.08 0.1
432.19 0.3
434.29 2.5
435.13 0.2
435.5 0.2
436.12 1.0

Annotations:

B^{2,5}X_{HexNAc}
437.44 0.4
438.21 21.5

Annotations:

C^{0,2}X_{HexNAc} ^{2,4}A_{Gal}
439.45 1.5

Annotations:

B^{1,3}X_{HexNAc}
446.97 0.2
448.09 0.3
450.48 0.4

Annotations:

B^{0,3}X_{HexNAc}
451.61 0.1
452.24 3.5

Annotations:

^{3,5}A_{Gal}
454.2 471.0

Annotations:

C^{2,5}X_{HexNAc}
455.19 67.9
456.33 11.0

Annotations:

^{0,3}X_{HexA}
457.22 0.8

Annotations:

C^{1,3}X_{HexNAc}
459.19 0.1
461.14 0.3
462.33 5.0
463.19 1.8
463.43 1.1
464.3 1.8
465.11 0.7
468.07 1.3

Annotations:

C^{0,3}X_{HexNAc} ^{0,3}A_{Gal} ^{1,4}A_{Gal} ^{0,4}X_{HexA}Z
468.38 0.7

Annotations:

C^{0,3}X_{HexNAc} ^{0,3}A_{Gal} ^{1,4}A_{Gal} ^{0,4}X_{HexA}Z
473.92 0.3
474.36 0.2
476.06 0.2
477.05 0.1
478.21 1.1
478.4 0.7
479.15 0.3
480.25 219.8

Annotations:

B^{0,4}X_{HexNAc} B^{2,4}X_{HexNAc} B^{1,3}X_{HexA} B^{0,4}X_{Gal} B^{1,3}X_{Gal}
481.22 36.0
481.77 0.9

Annotations:

^{1,3}X_{HexA}Z ^{2,5}A_{Gal} ^{0,4}X_{Gal}Z ^{1,3}X_{Gal}Z
482.39 2.2

Annotations:

^{1,3}X_{HexA}Z ^{2,5}A_{Gal} ^{0,4}X_{Gal}Z ^{1,3}X_{Gal}Z
483.08 0.5
484.29 0.9

Annotations:

C^{3,5}X_{HexNAc} C^{0,4}X_{HexA}
485.17 0.1
487.96 0.4
488.32 0.5
489.09 0.3
490.98 0.3
491.31 0.1
492.11 0.4
493.03 0.8
493.82 0.9
495.04 0.1
496.25 4.5
497.05 0.5
498.23 2945.6

Annotations:

C^{0,4}X_{HexNAc} C^{2,4}X_{HexNAc} C^{1,3}X_{HexA} C^{0,4}X_{Gal} C^{1,3}X_{Gal} ^{0,2}A_{Gal}
499.22 504.0
500.21 1.5

Annotations:

^{1,3}X_{HexA}Y ^{2,4}X_{HexA} ^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y
500.58 0.1

Annotations:

^{1,3}X_{HexA}Y ^{2,4}X_{HexA} ^{0,4}X_{Gal}Y ^{1,3}X_{Gal}Y
502.43 0.1
504.16 1.0
504.85 0.2
505.15 0.2
505.5 0.2
506.21 68.6
507.25 14.4
508.3 0.1
508.61 0.1
509.4 0.2
510.04 0.3
510.3 0.3
512.84 0.3
516.14 11.1
516.43 4.6
517.31 1.3
520.31 0.3
521.28 0.8
522.31 2.7
522.6 1.7
523.11 1.4
524.2 1448.8
525.23 259.6
526.24 1.6
526.58 0.4
527.33 0.5
527.79 0.3
528.04 0.2
528.86 0.4
539.19 1.0
540.21 17.6

Annotations:

B
541.18 14.0
542.23 9853.9

Annotations:

Z
543.23 1702.0
544.32 12.1
545.03 2.0
545.46 1.4
546.29 0.6
547.22 0.2
549.0 0.7
556.27 1.6
557.55 0.2
558.24 50.5

Annotations:

C
559.19 98.9
560.2 93.1

Annotations:

Y
561.25 88.5
562.24 19.9
562.82 0.3
563.14 0.4
564.32 0.3
565.83 0.3
568.95 0.2
571.42 0.1
573.0 0.3
578.14 0.1
580.09 0.2
581.05 1.6
581.3 2.6
584.28 1.8
585.14 0.1
585.37 0.2
589.1 0.5
590.21 0.1
591.05 0.4
591.82 0.3
594.16 0.4
595.98 0.1
597.25 0.1
597.81 0.2
599.1 0.7
599.34 0.2
600.34 0.7
600.77 1.7
601.23 8.0
601.67 0.7
602.13 0.5
602.32 0.3
602.67 0.2
604.34 0.3
606.11 0.3
609.9 0.2
611.5 0.6
612.16 0.3
613.45 0.3
616.29 0.5
617.02 0.2
618.05 0.2
619.2 0.7
620.1 0.6
622.39 0.1
623.18 0.1
624.26 0.2
625.21 0.6
627.08 0.1
628.59 0.2
629.39 0.4
632.79 0.3
633.26 0.3
634.28 0.1
634.78 0.2
635.38 0.2
639.66 0.3
643.16 1.3

Annotations:

^{0,2}X_{HexNAc}
644.06 0.2
644.55 0.2
649.96 0.2
651.16 0.3
658.19 0.1
659.24 0.7

Annotations:

^{2,5}X_{HexNAc}
660.12 1.0
660.79 0.5
663.28 0.6
664.02 0.1
666.97 0.1
668.5 0.3
672.6 0.4
677.09 1.3
677.37 0.5
677.94 0.1
679.25 0.1
682.27 0.3
683.2 0.7
683.58 0.4
683.86 0.5
684.16 0.4
685.35 0.2
686.15 0.6
686.51 1.0
687.03 0.3
687.31 0.1
688.08 0.2
688.87 0.2

Annotations:

^{3,5}X_{HexNAc} ^{0,4}X_{HexA}
689.34 0.3

Annotations:

^{3,5}X_{HexNAc} ^{0,4}X_{HexA}
691.51 0.8
692.35 0.2
695.82 1.1
697.99 1.3
701.34 2.2
702.24 13.8
703.27 4.3

Annotations:

^{0,4}X_{HexNAc} ^{2,4}X_{HexNAc} ^{1,3}X_{HexA} ^{0,4}X_{Gal} ^{1,3}X_{Gal}
703.82 0.7
704.14 0.6
704.35 0.6
706.54 0.1
708.33 0.4
709.52 0.3
713.38 0.2
714.24 6.2
715.05 0.8
715.34 3.0
716.11 0.2
716.39 0.3
717.31 0.9
718.06 0.2
718.35 0.8
719.28 63.0
720.31 12.5
721.24 3.1
722.29 0.3
725.96 2.5
726.28 6.4
727.3 1.8
728.03 0.1
728.39 0.6
728.7 0.3
729.34 1.9
732.51 0.6
732.77 0.4
733.9 0.3
735.45 0.2
736.64 0.2

There are 1 similar spectra:

Id Structure Score

233

0.94
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