Cross Validation of Liquid Chromatography-Mass Spectrometry and Lectin Array for Monitoring Glycosylation in Fed-Batch Glycoprotein Production.

Hayes CA, Doohan R, Kirkley D, Leister K, Harhen B, Savage AV, Karlsson NG., Mol Biotechnol., 2012, (51): 272 - 282

PubMed Entry:

Retention Time Recorded Precursor Mass Structure
25.1 730.2 [M -2H]2-
25.3 956.8 [M -2H]2-
25.4 1460.9 [M -2H]2-
25.6 811.3 [M -2H]2-
25.6 1037.9 [M -2H]2-
26.2 892.3 [M -2H]2-
27.2 1183.7 [M -2H]2-
27.3 1366.0 [M -2H]2-

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Cricetinae
Tissue - Ovary
Glycoprotein - CTAL4/IgG Fusion Protein

HPLC

Column Hypercarb

Method

  • 60 gradient with 10 run rate
  • 10mM Ammonium Bicarbonate
  • 80% Acetonitrile in 10mM Ammonium Bicarbonate

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

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