O-glycan repertoires on a mucin-type reporter protein expressed in CHO cell pools transiently transfected with O-glycan core enzyme cDNAs

PubMed Entry

GlyToucan Accession Number: G10831FE

Peak Intensity
250.25 0.6

Annotations:

^{1,5}X_{Gal}Y Y^{1,5}X_{GlcNAc}
251.27 0.2

Annotations:

C^{1,5}X_{NeuAc} C^{1,5}X_{NeuAc} ^{0,3}A_{GlcNAc}Y
272.08 0.6

Annotations:

^{2,5}X_{Gal}Z Z^{2,5}X_{Gal}
289.0 0.3

Annotations:

C^{3,5}X_{NeuAc}
289.98 12.4

Annotations:

B
290.28 6.5

Annotations:

B
290.98 0.7

Annotations:

^{1,4}A_{GlcNAc} ^{3,5}X_{Gal}Z C^{0,3}X_{Gal}
291.42 0.2

Annotations:

C^{0,3}X_{Gal} ^{1,4}A_{GlcNAc} ^{3,5}X_{Gal}Z
341.19 0.4
438.09 0.6

Annotations:

^{1,5}X_{NeuAc}Z Z^{1,4}X_{GlcNAc}
440.74 0.3

Annotations:

^{1,5}X_{NeuAc}Z Z^{1,5}X_{NeuAc}
445.07 0.5

Annotations:

Y^{2,4}X_{GlcNAc}
447.23 0.4

Annotations:

^{1,3}X_{NeuAc}Z Z^{1,3}X_{NeuAc}
448.03 0.5

Annotations:

^{1,3}X_{NeuAc}Z Z^{1,3}X_{NeuAc} ^{2,5}X_{NeuAc}Z Z^{2,5}X_{NeuAc}
464.9 0.5

Annotations:

Z^{2,5}X_{GlcNAc} ^{2,5}X_{Gal}Z Z^{2,5}X_{Gal}
470.67 0.3
472.18 0.7

Annotations:

^{3,5}X_{NeuAc}Y Y^{3,5}X_{NeuAc}
476.92 0.6
480.21 0.8

Annotations:

B^{3,5}X_{NeuAc} ^{0,4}X_{Gal}Z ^{0,4}X_{GlcNAc}Z Z^{0,4}X_{Gal} Z^{0,4}X_{GlcNAc} ^{0,4}X_{Gal}Z ^{0,4}X_{Gal}Z
495.06 0.3

Annotations:

Z^{3,5}X_{GlcNAc}
502.86 0.9
521.99 1.4
524.83 0.3
543.09 0.2
545.67 0.6
547.3 0.3

Annotations:

^{0,2}X_{Gal}Y Y^{0,2}X_{Gal}
551.22 0.4

Annotations:

ZZ ZZ
558.84 1.9

Annotations:

^{1,4}X_{Gal}Z Z^{1,4}X_{Gal}
559.13 1.2

Annotations:

^{1,4}X_{Gal}Z Z^{1,4}X_{Gal}
559.65 43.9

Annotations:

Y Y
560.16 23.0

Annotations:

Y Y
560.67 1.5

Annotations:

^{1,4}X_{NeuAc}Y
561.33 0.3
564.05 0.5
570.06 0.4

Annotations:

^{0,3}X_{NeuAc} ^{0,3}X_{NeuAc}
570.86 1.3
572.48 0.6

Annotations:

^{0,2}A_{GlcNAc} C^{2,4}X_{NeuAc}
577.26 0.3

Annotations:

^{1,4}X_{Gal}Y Y^{1,4}X_{Gal} ^{3,5}X_{NeuAc} ^{3,5}X_{NeuAc}
579.1 0.4
580.18 0.3
584.07 0.4
588.85 0.5

Annotations:

Z^{2,4}X_{GlcNAc} ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z Z^{1,3}X_{Gal} Z^{2,4}X_{Gal}
590.11 0.4
590.73 0.5
592.92 0.8
595.63 0.4

Annotations:

B^{0,4}X_{Gal} ^{1,5}X_{NeuAc} ^{1,5}X_{NeuAc}
600.96 0.4

Annotations:

^{3,5}X_{Gal}Y
601.37 0.3

Annotations:

^{3,5}X_{Gal}Y
607.58 0.6

Annotations:

Y^{2,4}X_{GlcNAc} ^{1,3}X_{Gal}Y ^{2,4}X_{Gal}Y Y^{1,3}X_{Gal} Y^{2,4}X_{Gal} ^{1,4}X_{NeuAc} ^{1,4}X_{NeuAc}
609.09 0.4
612.94 0.7

Annotations:

C^{0,4}X_{Gal} ^{1,3}X_{NeuAc}Z
614.94 0.3

Annotations:

^{1,3}X_{GlcNAc} ^{2,4}X_{NeuAc} ^{2,4}X_{NeuAc} B^{0,4}X_{NeuAc} Y^{1,5}X_{Gal} ^{0,4}X_{Gal}Y ^{1,5}X_{Gal}Y
616.85 0.4
617.2 1.2

Annotations:

^{3,5}X_{NeuAc} ^{3,5}X_{NeuAc}
620.58 0.5

Annotations:

B^{1,4}X_{NeuAc} ^{0,3}X_{Gal} ^{0,3}X_{Gal}
625.24 0.4
630.23 0.2
631.81 0.5
634.14 0.4

Annotations:

B^{2,4}X_{NeuAc}
635.31 0.7

Annotations:

^{0,4}X_{Gal} ^{0,4}X_{GlcNAc} ^{0,4}X_{Gal}
636.56 0.4

Annotations:

^{2,5}A_{GlcNAc}
642.27 0.5
643.06 0.4

Annotations:

^{1,3}X_{NeuAc} ^{1,3}X_{NeuAc}
644.38 0.5
648.26 0.3
657.51 0.4

Annotations:

Z
657.96 0.6
661.35 0.5

Annotations:

B^{3,5}X_{Gal}
662.11 0.9
662.39 1.0
669.36 0.4

Annotations:

^{0,3}X_{NeuAc}Z Z^{0,3}X_{NeuAc}
827.27 0.4

Annotations:

^{1,4}X_{GlcNAc}
828.86 3.4

Annotations:

YY
829.26 17.4

Annotations:

YY
830.06 1.1
830.31 1.8
830.57 0.6
994.97 0.3
1040.05 0.8

Annotations:

Y Y
1040.37 0.4

Annotations:

Y Y
1041.66 0.4
1056.34 0.6
1074.39 0.6
1076.18 0.4

Annotations:

^{1,3}X_{NeuAc}Y Y^{1,3}X_{NeuAc}
1077.46 0.5
1100.57 0.9
1117.52 1.3
1118.16 0.7
1118.48 1.1
1120.25 48.8

Annotations:

Y Y
1121.44 5.0
1121.84 1.5
1122.16 2.2
1261.6 0.3

There are 4 similar spectra:

Id Structure Score

2689

0.94

2922

0.92

2823

0.60

125

0.58
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