Cystic fibrosis and bacterial colonization define the sputum N-glycosylation phenotype

PubMed Entry

GlyToucan Accession Number: G28678RG

Peak Intensity
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Z
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B
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Y
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C
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YZ ZY
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B^{1,4}X_{NeuAc} ^{1,5}A_{Gal} C^{2,5}X_{Gal} ^{0,4}A_{Man}Y C^{0,2}X_{Gal}
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BY CZ YY
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YY
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B^{0,3}X_{Fuc} ^{1,4}A_{Man}Z
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C
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CZ CZ BY BY ZZ
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Z
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Z
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YZ ZY
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BZ YY
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BZ BY CZ
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BY CZ
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BZ
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CZ BY
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CY CY
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^{0,3}A_{GlcNAc}Z B^{1,4}X_{GlcNAc}
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CY
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C^{0,4}X_{NeuAc} ^{1,4}A_{GlcNAc}Z B^{0,3}X_{GlcNAc}
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Z
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YZ ZY
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C^{0,2}X_{NeuAc}
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^{0,2}X_{NeuAc}Z
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BY CZ BY CZ
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C^{0,3}X_{Fuc} C^{0,3}X_{Gal} ^{1,4}A_{GlcNAc}Y ^{2,5}X_{NeuAc}Y
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CY CY
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CY CY
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YZ ZY YY YY
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YY YY
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YY YY
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C^{1,5}X_{Fuc} C^{2,5}X_{NeuAc}
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C^{2,5}X_{NeuAc}
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YY
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YY
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C^{2,4}X_{NeuAc}
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CY
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B
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^{2,5}A_{GlcNAc}Y C^{1,3}X_{Fuc} C^{2,4}X_{Fuc} ^{0,2}A_{Man} ^{0,2}A_{GlcNAc}Y C^{0,4}X_{Gal} C^{1,3}X_{Gal} C^{2,4}X_{Gal}
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C^{0,4}X_{Fuc} C^{1,3}X_{NeuAc} ^{0,2}A_{GlcNAc}Y
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B^{2,4}X_{GlcNAc}
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BY CZ
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^{1,5}A_{Man}Y
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^{0,4}X_{Fuc}Y ^{1,3}X_{NeuAc}Y ^{1,5}X_{Gal}Y Y^{1,5}X_{Gal} ^{1,5}X_{Fuc}Y Y^{0,4}X_{Fuc} Y^{1,5}X_{Fuc} C^{1,5}X_{NeuAc} ^{2,5}X_{NeuAc}Y
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C^{1,5}X_{NeuAc} ^{2,5}X_{NeuAc}Y
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833.5 132.0

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^{0,2}X_{Gal}Y Y^{0,2}X_{Gal} Y^{0,2}X_{Fuc} ^{0,2}X_{Fuc}Y Y^{2,5}X_{GlcNAc} ^{2,5}X_{Gal}Y Y^{2,5}X_{Gal} C^{2,5}X_{NeuAc} ^{0,3}X_{NeuAc}Y
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CY
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BZ
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BZ
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ZZ ZZ
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Z^{2,4}X_{Fuc} Z^{2,4}X_{GlcNAc} ^{0,4}X_{Gal}Z Z^{0,4}X_{Gal} Z^{1,3}X_{Gal} Z^{2,4}X_{Gal} ^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z Z^{1,3}X_{Fuc}
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Y
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^{0,4}X_{Gal}Y Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} Y^{2,4}X_{GlcNAc} Y^{0,4}X_{Gal} Y^{1,3}X_{Gal} Y^{2,4}X_{Gal} ^{1,3}X_{Fuc}Y ^{2,4}X_{Fuc}Y ^{2,4}A_{GlcNAc}
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YY YY
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CY
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YZ ZY YZ ZY
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C^{2,4}X_{NeuAc}
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^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z Z^{1,5}X_{Gal} B^{0,3}X_{Fuc} B^{3,5}X_{Gal} Z^{1,3}X_{Fuc} Z^{2,4}X_{Fuc}
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B^{3,5}X_{Fuc} B^{0,4}X_{Fuc} B^{1,3}X_{NeuAc} Z^{0,2}X_{Fuc} ^{0,2}X_{Fuc}Z Z^{1,5}X_{GlcNAc} Z^{2,5}X_{Gal}
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Annotations:

C^{0,3}X_{Fuc} C^{3,5}X_{Fuc} ^{1,4}A_{Man}Y ^{0,4}X_{Fuc}Y ^{1,3}X_{NeuAc}Y ^{1,5}X_{Fuc}Y Y^{0,4}X_{Fuc} Y^{1,5}X_{Fuc}
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^{2,4}A_{GlcNAc}Y
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^{2,4}A_{GlcNAc}Y
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^{2,4}A_{GlcNAc}Y
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^{0,2}X_{NeuAc}Z ^{3,5}X_{GlcNAc}Z Z^{3,5}X_{GlcNAc}
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^{2,4}X_{GlcNAc}Z Z^{2,4}X_{GlcNAc}
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B^{0,4}X_{Fuc} B^{1,3}X_{NeuAc} ^{0,2}X_{NeuAc}Z Z^{0,2}X_{NeuAc} ^{2,5}X_{NeuAc}Z
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Annotations:

^{2,4}X_{GlcNAc}Y Y^{2,4}X_{GlcNAc} ^{0,2}X_{NeuAc}Y Z^{3,5}X_{Fuc} Y^{0,2}X_{NeuAc} ^{2,5}X_{NeuAc}Y ^{3,5}X_{Fuc}Z
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^{2,4}X_{GlcNAc}Y Y^{2,4}X_{GlcNAc} ^{0,2}X_{NeuAc}Y Z^{3,5}X_{Fuc} Y^{0,2}X_{NeuAc} ^{2,5}X_{NeuAc}Y ^{3,5}X_{Fuc}Z
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Y
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Y
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ZZ ZZ
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YZ ZY YZ ZY
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YZ ZY YZ ZY
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YY YY
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Z^{0,2}X_{GlcNAc}
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B
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^{1,4}X_{GlcNAc}
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YY
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Y
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Y
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Z^{0,2}X_{Fuc} ^{0,2}X_{Fuc}Z ^{0,4}X_{NeuAc}Z Z^{0,4}X_{NeuAc} ^{0,2}X_{Gal}Z Z^{0,2}X_{Gal} ^{0,2}A_{GlcNAc}Y
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C
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^{2,4}X_{NeuAc}Y Y^{2,4}X_{NeuAc}
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^{0,4}X_{Fuc}Z ^{1,3}X_{Fuc}Z ^{2,4}X_{Fuc}Z Z^{1,3}X_{Fuc} Z^{2,4}X_{Fuc} ^{1,3}X_{NeuAc}Z ^{0,4}X_{Gal}Z ^{1,3}X_{Gal}Z ^{2,4}X_{Gal}Z Z^{0,4}X_{Fuc} Z^{0,4}X_{Gal} Z^{1,3}X_{Gal} Z^{2,4}X_{Gal}
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Annotations:

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Annotations:

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Z
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^{3,5}X_{NeuAc} Y^{3,5}X_{GlcNAc}
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Z Z
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Y
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Y
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Y^{2,4}X_{GlcNAc}
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Annotations:

Y Y
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^{0,2}X_{Gal} ^{0,4}X_{NeuAc}
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^{0,2}X_{Gal} ^{0,4}X_{NeuAc}
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^{1,5}X_{Fuc} ^{1,5}X_{Fuc}
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BY CZ
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BY CZ
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YY
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^{2,4}X_{NeuAc}
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CY
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YZ ZY
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YZ ZY
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^{2,4}X_{Fuc} ^{1,3}X_{Fuc} ^{2,4}X_{Fuc} ^{0,4}X_{Gal} ^{1,3}X_{Gal} ^{2,4}X_{Gal} ^{1,3}X_{Fuc}
1082.5 305.0

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^{3,5}X_{Fuc} ^{3,5}X_{Fuc}
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^{3,5}X_{Fuc} ^{3,5}X_{Fuc}
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Y^{1,5}X_{GlcNAc}
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^{0,4}X_{Fuc} ^{0,4}X_{Fuc} ^{1,3}X_{NeuAc}
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BY CZ
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Y^{1,5}X_{Fuc}
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Annotations:

Y^{0,2}X_{Fuc} Y^{2,5}X_{GlcNAc} Y^{2,5}X_{Gal}
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^{0,2}A_{Man}Y
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Annotations:

Z^{1,3}X_{Fuc} Z^{2,4}X_{Fuc} Z^{2,4}X_{GlcNAc} Z^{0,4}X_{Gal} Z^{1,3}X_{Gal} Z^{2,4}X_{Gal}
1185.3 152.0

Annotations:

^{2,4}A_{GlcNAc}Y
1188.3 301.0
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Annotations:

Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} Y^{2,4}X_{GlcNAc} Y^{0,4}X_{Gal} Y^{1,3}X_{Gal} Y^{2,4}X_{Gal}
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Annotations:

ZZ
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Annotations:

Y^{0,4}X_{Fuc}
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Annotations:

CY
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Annotations:

ZY YZ
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Annotations:

ZZ
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Annotations:

Z
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Annotations:

C^{1,5}X_{Gal} ^{0,2}A_{Man}Y ^{0,2}A_{GlcNAc}Y
1258.3 262.0

Annotations:

YZ ZY
1258.5 290.0

Annotations:

YZ ZY
1259.6 515.0

Annotations:

^{2,4}A_{GlcNAc}Y C^{0,2}X_{Gal} ^{1,5}A_{Man}Y
1259.8 150.0

Annotations:

^{2,4}A_{GlcNAc}Y C^{0,2}X_{Gal} ^{1,5}A_{Man}Y
1260.7 303.0

Annotations:

Y
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1276.3 103.0

Annotations:

YY
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1302.4 241.0

Annotations:

^{2,5}X_{Gal}Y Y^{0,2}X_{Gal}
1302.6 180.0

Annotations:

^{2,5}X_{Gal}Y Y^{0,2}X_{Gal}
1328.0 224.0

Annotations:

BY CZ
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Annotations:

CY
1347.5 994.0

Annotations:

^{2,4}A_{GlcNAc}Y
1348.5 156.0

Annotations:

Y^{0,3}X_{Fuc} Y^{3,5}X_{Gal}
1348.7 166.0

Annotations:

Y^{0,3}X_{Fuc} Y^{3,5}X_{Gal}
1349.8 208.0
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Annotations:

Y^{1,3}X_{Fuc} Y^{2,4}X_{Fuc} Y^{0,4}X_{Gal} Y^{1,3}X_{Gal} Y^{2,4}X_{Gal}
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Annotations:

ZZ ZZ
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Annotations:

Y^{0,4}X_{Fuc}
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Annotations:

CZ BY
1403.7 190.0

Annotations:

^{2,4}A_{GlcNAc}Z
1404.4 142.0

Annotations:

Z
1407.6 141.0

Annotations:

^{0,2}A_{GlcNAc}Y
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1412.4 136.0
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Annotations:

^{2,4}A_{GlcNAc}Y
1422.2 204.0

Annotations:

Y
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Annotations:

Y
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Annotations:

^{0,2}X_{NeuAc}Z
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1463.0 372.0

Annotations:

C^{0,2}X_{Gal} ^{1,5}A_{GlcNAc}Y ^{1,5}A_{Man}Y
1464.6 231.0

Annotations:

^{0,2}A_{Man}Y ^{2,5}X_{Gal}Y
1472.5 193.0

Annotations:

BZ BZ
1478.5 182.0

Annotations:

C^{2,5}X_{Gal} ^{1,5}A_{Man}Y ^{1,5}X_{NeuAc}Y ^{0,3}X_{Gal}Y Y^{1,4}X_{GlcNAc}
1482.6 122.0
1490.6 281.0

Annotations:

BY CZ BY CZ
1505.6 222.0

Annotations:

Y^{2,5}X_{GlcNAc}
1508.7 129.0

Annotations:

CY CY
1517.8 130.0

Annotations:

Z^{3,5}X_{GlcNAc}
1530.7 422.0
1532.4 382.0

Annotations:

ZZ
1547.8 519.0
1548.3 163.0

Annotations:

^{2,4}A_{GlcNAc}Z B^{0,2}X_{Fuc} B^{2,5}X_{Gal}
1548.6 120.0

Annotations:

^{2,4}A_{GlcNAc}Z B^{0,2}X_{Fuc} B^{2,5}X_{Gal}
1548.8 129.0

Annotations:

^{2,4}A_{GlcNAc}Z B^{0,2}X_{Fuc} B^{2,5}X_{Gal}
1549.3 143.0

Annotations:

Y^{2,4}X_{GlcNAc}
1549.6 357.0

Annotations:

Y^{2,4}X_{GlcNAc}
1550.5 271.0

Annotations:

YZ ZY
1553.8 128.0
1559.5 286.0

Annotations:

^{0,4}X_{NeuAc}Z
1559.8 144.0

Annotations:

^{0,4}X_{NeuAc}Z
1560.7 413.0
1567.7 307.0
1568.4 157.0

Annotations:

YY
1569.5 319.0

Annotations:

C^{2,4}X_{NeuAc}
1570.7 121.0
1572.5 132.0
1572.7 159.0
1589.5 177.0

Annotations:

ZZ ZZ
1589.7 184.0

Annotations:

ZZ ZZ
1590.7 127.0
1591.6 167.0

Annotations:

YZ ZY
1601.5 143.0
1609.7 231.0

Annotations:

YY
1610.7 188.0

Annotations:

^{2,5}A_{GlcNAc}Y C^{1,3}X_{Fuc} C^{2,4}X_{Fuc} ^{0,2}A_{Man} ^{0,2}A_{GlcNAc}Y C^{0,4}X_{Gal} C^{1,3}X_{Gal} C^{2,4}X_{Gal}
1629.6 102.0
1634.4 172.0

Annotations:

B^{0,2}X_{NeuAc}
1652.5 630.0

Annotations:

B
1653.7 434.0

Annotations:

^{0,4}X_{Fuc}Y ^{1,3}X_{NeuAc}Y ^{1,5}X_{Gal}Y Y^{1,5}X_{Gal} ^{1,5}X_{Fuc}Y Y^{0,4}X_{Fuc} Y^{1,5}X_{Fuc}
1654.4 312.0

Annotations:

C^{1,5}X_{NeuAc} ^{2,5}X_{NeuAc}Y
1654.7 312.0

Annotations:

C^{1,5}X_{NeuAc} ^{2,5}X_{NeuAc}Y
1655.4 113.0
1658.9 123.0
1670.6 2525.0

Annotations:

C
1671.2 105.0
1671.6 2332.0
1672.6 1076.0
1673.6 466.0
1674.7 274.0
1678.5 119.0
1678.8 150.0
1695.6 291.0

Annotations:

YZ ZY YZ ZY
1695.8 313.0

Annotations:

YZ ZY YZ ZY
1696.6 1032.0
1697.8 278.0

Annotations:

Y
1698.9 108.0

Annotations:

C^{3,5}X_{NeuAc}
1701.3 262.0
1710.5 110.0
1711.5 154.0

Annotations:

CY
1712.6 355.0

Annotations:

^{2,4}A_{GlcNAc}
1713.6 627.0

Annotations:

YY YY
1714.7 655.0
1715.7 295.0
1716.1 234.0
1716.5 460.0
1717.6 117.0
1719.7 146.0
1720.6 226.0
1721.7 206.0

Annotations:

B^{1,5}X_{Gal} ^{0,4}X_{NeuAc}Z
1727.6 309.0

Annotations:

CY
1729.8 109.0
1730.5 261.0
1733.5 115.0
1734.6 133.0
1735.7 227.0

Annotations:

ZZ
1753.6 396.0

Annotations:

Z
1754.4 206.0

Annotations:

B^{2,4}X_{NeuAc}
1754.8 253.0

Annotations:

B^{2,4}X_{NeuAc}
1757.5 105.0
1769.9 276.0

Annotations:

ZY YZ ZY YZ
1770.6 110.0
1770.8 168.0
1771.7 449.0

Annotations:

Y
1772.5 206.0

Annotations:

C^{2,4}X_{NeuAc} ^{0,2}A_{GlcNAc}
1772.8 446.0

Annotations:

C^{2,4}X_{NeuAc} ^{0,2}A_{GlcNAc}
1773.4 154.0
1773.8 408.0
1774.9 178.0
1775.9 148.0
1796.5 104.0

Annotations:

Y^{0,2}X_{GlcNAc} ^{0,2}X_{GlcNAc}Y
1807.2 194.0
1813.5 149.0

Annotations:

C^{1,3}X_{Fuc} C^{2,4}X_{Fuc} C^{0,4}X_{Gal} C^{1,3}X_{Gal} C^{2,4}X_{Gal} ^{0,2}X_{Gal} Y^{0,2}X_{Gal}
1821.7 235.0
1822.8 197.0
1837.8 165.0
1839.7 117.0

Annotations:

^{0,2}X_{NeuAc}Z Z^{0,2}X_{NeuAc} ^{2,5}X_{NeuAc}Z
1855.7 926.0

Annotations:

B
1856.7 693.0

Annotations:

^{2,4}X_{GlcNAc}Y Y^{2,4}X_{GlcNAc}
1857.7 627.0

Annotations:

^{0,2}X_{NeuAc}Y Z^{3,5}X_{Fuc} Y^{0,2}X_{NeuAc} ^{2,5}X_{NeuAc}Y ^{3,5}X_{Fuc}Z
1858.4 176.0
1858.8 189.0
1859.4 116.0

Annotations:

Y
1873.6 302.0

Annotations:

C
1873.8 458.0

Annotations:

C
1874.7 157.0
1875.4 101.0

Annotations:

Y^{3,5}X_{Fuc} ^{3,5}X_{Fuc}Y
1875.8 175.0

Annotations:

Y^{3,5}X_{Fuc} ^{3,5}X_{Fuc}Y
1880.7 350.0

Annotations:

ZZ ZZ
1881.5 131.0
1881.8 211.0
1882.8 263.0
1883.6 176.0
1883.9 116.0
1923.6 140.0
1930.8 115.0
1931.7 137.0

Annotations:

^{1,4}X_{GlcNAc}
1932.8 645.0

Annotations:

YY
1933.4 317.0

Annotations:

Y
1933.8 1945.0

Annotations:

Y
1934.6 1056.0
1934.9 735.0
1935.7 714.0
1936.7 232.0
1936.9 420.0
1937.7 235.0
1968.8 391.0
2046.8 102.0

There are 2 similar spectra:

Id Structure Score

2389

0.51

2391

0.50
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